AB13507

Anti-ERp57抗体

Name: ERp57抗体 (ab13507)| Abcam中文官网
Brand: Abcam Limited
SKU: AB13507
Price: 5001 CNY
Availability: InStock
Rating: 5 (6 reviews)

Anti-ERp57 antibody

(6 Reviews)

(11 Publications)

Rabbit Polyclonal ERp57 antibody. Suitable for WB, ICC/IF and reacts with Chinese hamster, Mouse, Rat, Human samples. Cited in 11 publications.

查看别名

ERP57, ERP60, GRP58, PDIA3, Protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, Endoplasmic reticulum resident protein 60, p58, ER protein 57, ERp57, ER protein 60, ERp60

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (AB13507)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (AB13507)

ICC/IF image of ab13507 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13507, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ICC/IF

AbReview39385****

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (AB13507)

ab13507 staining ERp57 in the Human PC-3 prostate cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in 1% Donkey serum in PBST) for 1 hour at 22°C. An undiluted Alexa Fluor® 594-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Armen Petrosyan

AbReview39384****

Western blot - Anti-ERp57 antibody (AB13507)

All lanes:

Western blot - Anti-ERp57 antibody (ab13507) at 1/2000 dilution

All lanes:

Human LNCaP whole cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated Donkey anti-rabbit at 1/10000 dilution

Predicted band size: 57 kDa

Observed band size: 55 kDa

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Exposure time: 45s

This image is courtesy of an Abreview submitted by Armen Petrosyan

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Western blot - Anti-ERp57 antibody (AB13507)

All lanes:

Western blot - Anti-ERp57 antibody (ab13507) at 1/1000 dilution

Lane 1:

Molecular weight marker

Lane 2:

Cell lysates prepared from CHO-K1 cells

Lane 3:

Cell lysates prepared from human liver microsomes

Lane 4:

Cell lysates prepared from rat liver microsomes

Lane 5:

Cell lysates prepared from mouse liver microsomes

Predicted band size: 57 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human, Chinese hamster

应用

WB, ICC/IF

applications

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "This dilution detects Erp57 in 20µg of rat liver microsomal preparation by ECL analysis.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "This dilution detects Erp57 in 20µg of rat liver microsomal preparation by ECL analysis.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "This dilution detects Erp57 in 20µg of rat liver microsomal preparation by ECL analysis.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "This dilution detects Erp57 in 20µg of rat liver microsomal preparation by ECL analysis.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ERp57 also known as 4f9 is a protein responsible for catalyzing disulfide bond formations and isomerizations in the endoplasmic reticulum. ERp57 has a molecular mass of approximately 57 kDa and belongs to the protein disulfide isomerase (PDI) family. Its main expression occurs in the endoplasmic reticulum of cells where it plays a role in ensuring proper protein folding and quality control.

Biological function summary

ERp57 interacts with calnexin and calreticulin to form a complex involved in glycoprotein folding. This complex assists in the proper assembly and maturation of nascent glycoproteins by ensuring correct folding pathways within the cell. ERp57 has a specialized role in managing oxidative protein folding which makes it essential for cell function and homeostasis.

Pathways

ERp57 participates in the calnexin/calreticulin cycle which is critical for protein triage in the endoplasmic reticulum. This cycle ensures that only properly folded proteins progress to their final destinations. ERp57 also interacts with ERp72 another member of the PDI family to facilitate thiol-disulfide exchange reactions. These interactions position ERp57 as a central player in the maintenance of cellular redox balance and protein homeostasis.

Alterations in ERp57 function can relate to neurodegenerative diseases such as Alzheimer's and certain types of cancer. Misfolded proteins often accumulate in these conditions highlighting ERp57's role in preventing cellular stress. Moreover ERp57 associates with the protein tau in Alzheimer's disease where it assists in tau’s proper folding and diminishes its aggregation tendency. Additionally disruptions in the ERp57 pathway may interact with cancer-related proteins like p53 modulating apoptosis and cell cycle regulation.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone (PubMed : 11825568, PubMed : 16193070, PubMed : 27897272, PubMed : 36104323, PubMed : 7487104). Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity (PubMed : 35948544, PubMed : 36104323).

See full target information PDIA3

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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 2:100456 PubMed34751258

2021

SARS-CoV-2 can infect and propagate in human placenta explants.

Applications

Unspecified application

Species

Unspecified reactive species

Amal Fahmi,Melanie Brügger,Thomas Démoulins,Beatrice Zumkehr,Blandina I Oliveira Esteves,Lisamaria Bracher,Carlos Wotzkow,Fabian Blank,Volker Thiel,David Baud,Marco P Alves

Scientific reports 6:22279 PubMed26931299

2016

The fetal ovary exhibits temporal sensitivity to a 'real-life' mixture of environmental chemicals.

Applications

IHC

Species

Unspecified reactive species

Richard G Lea,Maria R Amezaga,Benoit Loup,Béatrice Mandon-Pépin,Agnes Stefansdottir,Panagiotis Filis,Carol Kyle,Zulin Zhang,Ceri Allen,Laura Purdie,Luc Jouneau,Corinne Cotinot,Stewart M Rhind,Kevin D Sinclair,Paul A Fowler

Proteome science 13:18 PubMed26155198

2015

Discovery of porcine maternal factors related to nuclear reprogramming and early embryo development by proteomic analysis.

Applications

Species

Unspecified reactive species

Qi Zhao,Zheng Guo,Shanhua Piao,Chunsheng Wang,Tiezhu An

The Journal of biological chemistry 290:6256-69 PubMed25605727

2015

Keratin 1 plays a critical role in golgi localization of core 2 N-acetylglucosaminyltransferase M via interaction with its cytoplasmic tail.

Applications

Unspecified application

Species

Unspecified reactive species

Armen Petrosyan,Mohamed F Ali,Pi-Wan Cheng

Molecular cancer research : MCR 12:1704-16 PubMed25086069

2014

Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Armen Petrosyan,Melissa S Holzapfel,David E Muirhead,Pi-Wan Cheng

Reproduction (Cambridge, England) 148:367-75 PubMed25030891

2014

Identification and characterization of an oocyte factor required for sperm decondensation in pig.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyu Li,Yanjun Huan,Bingteng Xie,Jiaqiang Wang,Yanhua Zhao,Mingxia Jiao,Tianqing Huang,Qingran Kong,Zhonghua Liu

Reproduction (Cambridge, England) 147:599-614 PubMed24446454

2014

Proteomic analysis of the sheep caruncular and intercaruncular endometrium reveals changes in functional proteins crucial for the establishment of pregnancy.

Applications

WB, IHC-P

Species

Sheep, Sheep

K H Al-Gubory,M Arianmanesh,C Garrel,S Bhattacharya,P Cash,P A Fowler

Andrology 2:259-66 PubMed24478030

2014

Paternal exposure to testis cancer chemotherapeutics alters sperm fertilizing capacity and affects gene expression in the eight-cell stage rat embryo.

Applications

Unspecified application

Species

Unspecified reactive species

J Maselli,B F Hales,B Robaire

PloS one 6:e22172 PubMed21799787

2011

Microinjection manipulation resulted in the increased apoptosis of spermatocytes in testes from intracytoplasmic sperm injection (ICSI) derived mice.

Applications

WB, IHC-P

Species

Mouse, Mouse

Yang Yu,Chun Zhao,Zhuo Lv,Wen Chen,Man Tong,Xuejiang Guo,Liu Wang,Jiayin Liu,Zuomin Zhou,Hui Zhu,Qi Zhou,Jiahao Sha

Proteomics 10:2620-30 PubMed20461717

2010

Proteomic analysis of colonic mucosa in a rat model of irritable bowel syndrome.

Applications

Species

Rat

Ying Ding,Bin Lu,Dongbo Chen,Lina Meng,Yan Shen,Shuo Chen

View all publications

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Anti-ERp57抗体

Anti-ERp57 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (AB13507)

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (AB13507)

Western blot - Anti-ERp57 antibody (AB13507)

Western blot - Anti-ERp57 antibody (AB13507)