Anti-ERp57抗体

• WB

AbReview39384****

Western blot - Anti-ERp57 antibody (AB13507)

All lanes:

Western blot - Anti-ERp57 antibody (ab13507) at 1/2000 dilution

All lanes:

Human LNCaP whole cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated Donkey anti-rabbit at 1/10000 dilution

Predicted band size: 57 kDa

Observed band size: 55 kDa

true

Exposure time: 45s

This image is courtesy of an Abreview submitted by Armen Petrosyan

• ICC/IF

AbReview39385****

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (AB13507)

ab13507 staining ERp57 in the Human PC-3 prostate cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in 1% Donkey serum in PBST) for 1 hour at 22°C. An undiluted Alexa Fluor® 594-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Armen Petrosyan

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (AB13507)

ICC/IF image of ab13507 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13507, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• WB

Unknown

Western blot - Anti-ERp57 antibody (AB13507)

All lanes:

Western blot - Anti-ERp57 antibody (ab13507) at 1/1000 dilution

Lane 1:

Molecular weight marker

Lane 2:

Cell lysates prepared from CHO-K1 cells

Lane 3:

Cell lysates prepared from human liver microsomes

Lane 4:

Cell lysates prepared from rat liver microsomes

Lane 5:

Cell lysates prepared from mouse liver microsomes

Predicted band size: 57 kDa

false