重组Anti-ERp29抗体[EPR12499(B)] (ab176573)
![Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody [EPR12499(B)] (ab176573)](https://www.abcam.cn/ps/products/176/ab176573/Images/ab176573-521707-anti-erp29-antibody--epr12499-b-immunocytochemistry-hepg2-cells-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody [EPR12499(B)] (ab176573)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12499(B)] to ERp29
- Suitable for: IHC-P, WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ERp29抗体[EPR12499(B)]
参阅全部 ERp29 一抗 -
描述
兔单克隆抗体[EPR12499(B)] to ERp29 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken, Cow
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免疫原
Synthetic peptide within Human ERp29 aa 50-150 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
Database link: P30040 -
阳性对照
- WB: HEK293T, HeLa, HepG2, MCF7 and OVCAR-3 cell lysates. ICC/IF: HepG2 cells. IHC-P: Human kidney and liver tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12499(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176573于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
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| ICC/IF |
1/100 - 1/250.
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| 说明 |
|---|
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 29 kDa. |
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ICC/IF
1/100 - 1/250. |
靶标
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功能
Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER. -
组织特异性
Ubiquitous. Mostly expressed in secretory tissues. -
细胞定位
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 416882 Chicken
- Entrez Gene: 613357 Cow
- Entrez Gene: 10961 Human
- Entrez Gene: 67397 Mouse
- Entrez Gene: 117030 Rat
- Omim: 602287 Human
- SwissProt: P81628 Chicken
- SwissProt: P81623 Cow
see all -
别名
- C12orf8 antibody
- Chromosome 12 open reading frame 8 antibody
- Endoplasmic reticulum lumenal protein ERp28 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
Immunocytochemistry/ Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling ERp29 with ab176573 at 1:50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution (2 µg/ml). Cells were fixed in 100% methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (2.5 µg/ml). DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal image showing cytoplasmic staining in HepG2 cell line.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). -
![Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573)](https://www.abcam.cn/ps/products/176/ab176573/Images/ab176573-384456-anti-erp29-antibody-epr12499b-western-blot-hek293t-hepg2.jpg)
Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573)All lanes : Anti-ERp29 antibody [EPR12499(B)] (ab176573) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ERP29 knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDaLanes 1-3: Merged signal (red and green). Green - ab176573 observed at 29 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab176573 Anti-ERp29 antibody [EPR12499(B)] was shown to specifically react with ERp29 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266543 (knockout cell lysate ab257188) was used. Wild-type and ERp29 knockout samples were subjected to SDS-PAGE. ab176573 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573)](https://www.abcam.cn/ps/products/176/ab176573/Images/ab176573-204289-anti-erp29-antibody-epr12499b-western-blot.jpg)
Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573)All lanes : Anti-ERp29 antibody [EPR12499(B)] (ab176573) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : OVCAR-3 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 29 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (ab176573)](https://www.abcam.cn/ps/products/176/ab176573/Images/ab176573-204291-anti-erp29-antibody-epr12499b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (ab176573)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ERp29 using ab176573 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (ab176573)](https://www.abcam.cn/ps/products/176/ab176573/Images/ab176573-204292-anti-erp29-antibody-epr12499b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (ab176573)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ERp29 using ab176573 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-ERp29 antibody [EPR12499(B)] (ab176573)](https://www.abcam.cn/ps/products/176/ab176573/Images/ab176573-6-benefits-of-recombinant-antibodies.png)
Anti-ERp29 antibody [EPR12499(B)] (ab176573)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab176573 被引用在 5 文献中.
- Liu F et al. Dexmedetomidine alleviates insulin resistance in hepatocytes by reducing endoplasmic reticulum stress. Endocrine 67:87-94 (2020). PubMed: 31679138
- Carron J et al. Role of a genetic variation in the microRNA-4421 binding site of ERP29 regarding risk of oropharynx cancer and prognosis. Sci Rep 10:17039 (2020). PubMed: 33046743
- Zou S et al. ERp29 affects the migratory and invasive ability of human extravillous trophoblast HTR-8/SVneo cells via modulating the epithelial-mesenchymal transition. J Biochem Mol Toxicol 34:e22454 (2020). PubMed: 31981282
- Ye W et al. ERp29 downregulation enhances lung adenocarcinoma cell chemosensitivity to gemcitabine by upregulating HSP27 phosphorylation. Exp Ther Med 17:817-823 (2019). PubMed: 30651868
- Chaiyawat P et al. Endoplasmic reticulum protein 29 (ERp29) as a novel prognostic marker and tumor suppressor in osteosarcoma. J Bone Oncol 16:100233 (2019). PubMed: 30984555