重组Anti-Erlin-2抗体[EPR8088] (ab129207)
![Western blot - Anti-Erlin-2 antibody [EPR8088] (ab129207)](https://www.abcam.cn/ps/products/129/ab129207/Images/ab129207-143278-anti-erlin-2-antibody-epr8088-western-blot.jpg "Western blot - Anti-Erlin-2 antibody [EPR8088] (ab129207)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8088] to Erlin-2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Erlin-2抗体[EPR8088]
参阅全部 Erlin-2 一抗 -
描述
兔单克隆抗体[EPR8088] to Erlin-2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide within Human Erlin-2 aa 50-150. The exact sequence is proprietary.
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阳性对照
- Fetal kidney, 293T, HepG2, and HeLa lysates; Human endometrium adenocarcinoma; HepG2 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 5.40 x 10 -13 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8088 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129207于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/100 - 1/250.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/250. |
应用说明
Is unsuitable for Flow Cyt or IP.
靶标
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功能
Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Also involved in ITPR1 degradation by the ERAD pathway. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the band 7/mec-2 family. -
细胞定位
Endoplasmic reticulum membrane. Associated with lipid raft-like domains of the endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 11160 Human
- Entrez Gene: 244373 Mouse
- Omim: 611605 Human
- SwissProt: O94905 Human
- SwissProt: Q8BFZ9 Mouse
- Unigene: 705490 Human
- Unigene: 277699 Mouse
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别名
- C8orf2 antibody
- Chromosome 8 open reading frame 2 antibody
- Endoplasmic reticulum lipid raft associated protein 2 antibody
see all
图片
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Western blot - Anti-Erlin-2 antibody [EPR8088] (ab129207)All lanes : Anti-Erlin-2 antibody [EPR8088] (ab129207) at 1/1000 dilution
Lane 1 : Fetal kidney tissue lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 38 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erlin-2 antibody [EPR8088] (ab129207)](https://www.abcam.cn/ps/products/129/ab129207/Images/ab129207-143277-anti-erlin-2-antibody-epr8088-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erlin-2 antibody [EPR8088] (ab129207)ab129207, at 1/50 dilution, staining Erlin-2 in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Erlin-2 antibody [EPR8088] (ab129207)](https://www.abcam.cn/ps/products/129/ab129207/Images/ab129207-143276-anti-erlin-2-antibody-epr8088-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Erlin-2 antibody [EPR8088] (ab129207)ab129207, at 1/100 dilution, staining Erlin-2 in HepG2 cells by Immunofluorescence.
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![OI-RD Scanning - Anti-Erlin-2 antibody [EPR8088] (ab129207)](https://www.abcam.cn/ps/products/129/ab129207/Images/ab129207-143275-anti-erlin-2-antibody-epr8088.jpg)
OI-RD Scanning - Anti-Erlin-2 antibody [EPR8088] (ab129207) -
![Anti-Erlin-2 antibody [EPR8088] (ab129207)](https://www.abcam.cn/ps/products/129/ab129207/Images/ab129207-5-benefits-of-recombinant-antibodies.png)
Anti-Erlin-2 antibody [EPR8088] (ab129207)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab129207 被引用在 3 文献中.
- Agrawal RR et al. Alzheimer's-Associated Upregulation of Mitochondria-Associated ER Membranes After Traumatic Brain Injury. Cell Mol Neurobiol N/A:N/A (2022). PubMed: 36571634
- Rossi AM et al. Quantal Ca2+ release mediated by very few IP3 receptors that rapidly inactivate allows graded responses to IP3. Cell Rep 37:109932 (2021). PubMed: 34731613
- Liu Y et al. Molecular and Immune Characteristics for Lung Adenocarcinoma Patients With ERLIN2 Overexpression. Front Immunol 11:568440 (2020). PubMed: 33424830