AB232748

Anti-ERK5抗体

Name: ERK5抗体 (ab232748)| Abcam中文官网
Brand: Abcam Limited
SKU: AB232748
Price: 4748 CNY
Availability: InStock

Anti-ERK5 antibody

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(1 Publication)

Rabbit Polyclonal ERK5 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MAPK7 aa 550-800.

查看别名

BMK1, ERK5, PRKM7, MAPK7, Mitogen-activated protein kinase 7, MAP kinase 7, MAPK 7, Big MAP kinase 1, Extracellular signal-regulated kinase 5, BMK-1, ERK-5

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

Paraffin-embedded human colorectal cancer tissue stained for ERK5 using ab232748 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

Paraffin-embedded human lung cancer tissue stained for ERK5 using ab232748 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

Paraffin-embedded human kidney tissue stained for ERK5 using ab232748 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-ERK5 antibody (AB232748)

All lanes:

Western blot - Anti-ERK5 antibody (ab232748) at 2 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 88 kDa

false

Supplier Data

Western blot - Anti-ERK5 antibody (AB232748)

All lanes:

Western blot - Anti-ERK5 antibody (ab232748) at 2 µg/mL

All lanes:

Recombinant human ERK5 protein

Predicted band size: 26 kDa,88 kDa

false

Supplier Data

Western blot - Anti-ERK5 antibody (AB232748)

All lanes:

Western blot - Anti-ERK5 antibody (ab232748) at 2 µg/mL

All lanes:

Pig colon tissue lysate

Predicted band size: 88 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human, Pig

应用

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human MAPK7 aa 550-800. The exact immunogen used to generate this antibody is proprietary information.

Q13164

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab232748 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ERK5 also known as big MAP kinase 1 (BMK1) is an enzyme that functions as a protein-serine/threonine kinase within the MAP kinase family. It has a molecular weight of approximately 120 kDa. ERK5 is expressed in various tissues including the brain heart and lungs with notable expression in endothelial cells. The protein plays a mechanical role in signal transduction processes which it achieves by transmitting signals from extracellular sources to the cellular nucleus facilitating the regulation of gene expression.

Biological function summary

ERK5 influences cellular functions through its role in promoting cell proliferation and differentiation. It serves as part of a signaling complex that includes the MAP kinase kinase 5 (MEK5). This interaction allows ERK5 to phosphorylate and subsequently activate target substrates such as transcription factors promoting cellular responses necessary for development and survival. ERK5 also plays a role in the response to oxidative stress which contributes to its impact on survival pathways and cellular stress responses.

Pathways

ERK5 acts within the MAPK signaling cascade and is linked to the ERK/MAPK pathway and the phosphoinositide 3-kinase (PI3K) pathway. Within these pathways ERK5 interacts with other MAP kinases and regulatory proteins such as Ras and Raf contributing to a wide range of cellular processes including growth migration and survival. Through these interactions ERK5 influences cellular dynamics and mediates responses to growth factors and stress stimuli.

Abnormal activity of ERK5 has been implicated in various conditions including cardiovascular disease and cancer. Elevated ERK5 signaling can promote tumor progression and metastasis in certain cancers by enhancing tumor cell proliferation and survival. In cardiovascular disorders ERK5 interacts with proteins like calcium/calmodulin-dependent protein kinase II (CaMKII) influencing pathological cardiac remodeling and hypertrophy. These associations highlight ERK5's impact on disease mechanisms and highlight its potential as a therapeutic target.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via interaction with STUB1/CHIP and promotion of STUB1-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity). May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.

See full target information MAPK7

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 637:402-411 PubMed39633051

2024

Fetal hepatocytes protect the HSPC genome via fetuin-A.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Lin Guo,Yi-Ding Wang,Yan-Jun Liu,Lei Chu,Hua Zhu,Ye Hu,Ren-Yan Wu,Hong-Yu Xie,Juan Yu,Shui-Ping Li,Chao-Yang Xiong,Ruo-Yan Li,Fang Ke,Lei Chen,Guo-Qiang Chen,Liang Chen,Fan Bai,Tariq Enver,Guo-Hong Li,Huai-Fang Li,Deng-Li Hong

View all publications

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Anti-ERK5抗体

Anti-ERK5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

Western blot - Anti-ERK5 antibody (AB232748)

Western blot - Anti-ERK5 antibody (AB232748)

Western blot - Anti-ERK5 antibody (AB232748)