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Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187)抗体[MAPK-YT] (ab50011)

Reviews (14)Q&A (8)References (120)
Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011)
  • Immunocytochemistry/ Immunofluorescence - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011)
  • Immunocytochemistry/ Immunofluorescence - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011)

Key features and details

  • Mouse monoclonal [MAPK-YT] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
  • Suitable for: ICC/IF, WB
  • Reacts with: Rat, Saccharomyces cerevisiae, Drosophila melanogaster
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187)抗体[MAPK-YT]
    参阅全部 Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 一抗

  • 描述

    小鼠单克隆抗体[MAPK-YT] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC/IF, WBmore details

  • 种属反应性

    与反应: Rat, Saccharomyces cerevisiae, Drosophila melanogaster
    预测可用于: Xenopus laevis, Zebrafish

  • 免疫原

    Synthetic peptide corresponding to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide corresponds to the phosphorylated form of human ERK1 and ERK2 activation loop.
    Sequence:

    HTGFLpTEpYVAT

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 表位

    The epitope recognized by ab50011 is located in the region of phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase (Thr185 and Tyr187 in ERK-2).

  • 阳性对照

    • Rat brain extract, Drosophila wing imaginal disc.

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot is from ascites and is still in stock as of 25th October 2016- GR185138. Lot numbers higher than GR185138 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab50011于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (2)
1/200.
WB (8)
1/10000. Predicted molecular weight: 44 kDa.
说明
ICC/IF
1/200.
WB
1/10000. Predicted molecular weight: 44 kDa.

图片

  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011)
    Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011)
    Lane 1 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011) at 1/10000 dilution
    Lane 2 : no primary antibody

    All lanes : Extract of Rat1 cells activated with 100µM
    Vanadate+200µM H2O2

    Secondary
    All lanes : Goat Anti-Mouse IgG, AlkPhos
    Conjugate

    Predicted band size: 44 kDa
    Observed band size: 42,44 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011)
    Immunocytochemistry/ Immunofluorescence - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011)
    In situ immunostaining of the activated form of MAP kinase with ab50011 (green) in Drosophila wing imaginal disc (500 micrometers long).
  • Immunocytochemistry/ Immunofluorescence - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011)
    Immunocytochemistry/ Immunofluorescence - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011)

    ICC/IF of NRK cells labeling the activated form of MAP kinase with ab50011 (1/1000 dilution). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue).

文献 (120)

发表研究结果有使用 ab50011？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab50011 被引用在 120 文献中.

  • Wu X  et al. Multi-modal imaging for uptake of peptide ligand specific for CD44 by hepatocellular carcinoma. Photoacoustics 26:100355 (2022). PubMed: 35479192
  • Liu C  et al. Disrupted tenogenesis in masseter as a potential cause of micrognathia. Int J Oral Sci 14:50 (2022). PubMed: 36257937
  • Li N  et al. Altered BMP-Smad4 signaling causes complete cleft palate by disturbing osteogenesis in palatal mesenchyme. J Mol Histol 52:45-61 (2021). PubMed: 33159638
  • Tao J  et al. Sfrp1 protects against acute myocardial ischemia (AMI) injury in aged mice by inhibiting the Wnt/ß-catenin signaling pathway. J Cardiothorac Surg 16:12 (2021). PubMed: 33468190
  • Zhang C  et al. Anti-Inflammatory Effect Fraction of Bletilla striata and Its Protective Effect on LPS-Induced Acute Lung Injury. Mediators Inflamm 2021:6684120 (2021). PubMed: 33776576
View all Publications for this product

客户评价及客户问答

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1-10 of 22 Abreviews or Q&A

Western blot abreview for Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12 cell)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
PC12 cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Ji Yeon Lee

Verified customer

提交于 Jan 25 2019

Western blot abreview for Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Total protein from zebrafish testicle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Treatment
Animals exposed to bisphenol A during 21 days
Specification
Total protein from zebrafish testicle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 15 2018

Immunohistochemistry (Frozen sections) abreview for Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Xenopus laevis Tissue sections (Tadpole limb)
Permeabilization
Yes - 1% Triton X-100
Specification
Tadpole limb
Blocking step
Roche blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Gufa Lin

Verified customer

提交于 Sep 12 2017

Immunohistochemistry (Frozen sections) abreview for Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT]

 Poor
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Aceton methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 06 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) [MAPK-YT] antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako REALPeroxidase-Blocking Solution S2023 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Antigen retrieval step
Heat mediated
Sample
Zebrafish Tissue sections (Liver, dysplatic cells)
Specification
Liver, dysplatic cells
Permeabilization
No
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Inhye Jung

Verified customer

提交于 Jul 17 2014

Question

Hi again

Thanks for your reply. When you say that the total concentration antibody is 3-6 mg/ml is that only the specific antibody? or is there also contamination of other antibodies, and how much?

Best regards

Read More
Answer

Thank you for your reply and further question.

This monoclonal antibody in the ascites fluid that was produced by the hybridoma within the mouse peritoneal cavity. As far as I can tell there should only be the specific antibody present in terms of immunoglobulins, however as this is a (relatively clean) bodily fluid, other mouse proteins will be present.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact me.

Read More

Question

Hi Abcam

I have a question concerning your product: "Anti-ERK1 + ERK2 (phospho T185 + Y187 + T202 + Y204) antibody [MAPK-YT] (ab50011)"

https://www.abcam.com/ERK1-ERK2-phospho-T185-Y187-204-T202-antibody-MAPK-YT-ab50011.html

Do you have data on the affinity of the antibody-ERK, antibody-pERK and antibody-immunogen interactions?

And do you know what the concentration of antibody is in the ascites?

Best regards

Read More
Answer

Thank you for your enquiry.

I can confirm that the total protein concentration in ab50011 should be between 15 - 55 mg/ml when measured by Biuret and that the IgG1 concentration should be between 3 - 6 mg/ml.

Regrettably we do not have any other information regarding affinity and antibody immunogen interactions. Unfortunately, it is unusual to have this type of information for research grade antibodies.

I am sorry we do not have all the information you require on this occasion, however I hope it is still helpful. If you have any further questions, please do not hesitate to contact us.

Read More

Question

As we have very limited time to get this Ab working to our satisaction, I'd be happy for you to send me a vial from the batch you currently have available.
Thanks,

Read More
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of a different lot number with the order number 1140603.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Question

Thanks for your response.
I think we would like to proceed by taking you up on your offer of a free vial of GR121-7.
If as we hope, we replicate our original results, would we subsequently be able to order 4 more vials to complete our study?
Best wishes,

Read More
Answer

Thank you for your reply.

I am very sorry to confirm that we sell a lot of this antibody and regrettably we now have no vials of GR121-7 left.

As previously discussed, I can confirm that our lots GR121-7 and GR121-8 are the same original batch from the producer, the vials have just come to us in different deliveries. I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. Regrettably it seems you received a bad vial on this occasion.

However, I do fully understand your concerns, and would be pleased to provide you with a free vial from a completely different lot to try. Or I can provide a credit note.

I am sorry your prefered batch ran out of stock so quickly. Thank you for your understanding. I look forward to hearing from you with details of how you would like to proceed in this case.

Read More

Question

Order Details
Antibody code: ab50011
Lot number:GR121-8
Purchase order number
or preferably Abcam order number: Purchase order number is ####
General Information
Antibody storage conditions (temperature/reconstitution etc)
Stored at 40C
Description of the problem (high background, low signal, non-specific
satining etc.)
This batch gave no nuclear staining as expected, when we worked up the
Ab using GR121-7. All staining was cytoplasmic with a small percentage
of membranous.
Sample (Species/Tissue/Cell Type/Cell Line etc.) Human colorectal
tumour tissue
Fixation of sample
(Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)
Formalin fixed, and paraffin embedded.
Antigen retrieval (Enzymatic method, Heat mediated technique etc.)
Vector Antigen unmasking solution, 2 minutes in a pressure cooker
Permeabilization step
Blocking conditions (Buffer/time period, Blocking agent etc.) 15
minutes in hydrogen peroxide, and 10 minute is 1x casein block
Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation
time, Wash step)
Ab50011 GR121-8, 1 in 50 dilution, diluted in Zymed Antibody diluents
containing 1:50 dilution of casein. Incubated 16 hours at 4 degrees C.
Washed off with TBS.
Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation
time, Wash step)
DAKO envision plus mouse secondary HRP-polymer, for 30 minutes. Again
washed off with TBS.
Detection method
DAB, followed by counterstaining with Hematoxylin and lithium carbonate.
Positive and negative controls used (please specify)
Human kidney sections used.
Optimization attempts (problem solving)
How many times have you tried the IHC?
We optimised lot number GR121-7, over several runs, changing dilution
factor, retrieval method, secondary Ab type etc, but when GR121-8 was
run using the identical conditions used to optimise GR121-7, we saw NO
nuclear staining as expected, and previously demonstrated. We have
repeated the GR121-8 run and saw exactly the same staining pattern as
seen previously, where NO nuclear staining was detected.
Have you run a "No Primary" control?
Yes
Do you obtain the same results every time?
Yes
What steps have you altered? NO steps were altered between runs using
GR121-7 and GR121-8, so we expected to have obtained identical
staining patterns.
Additional Notes
We would appreciate if you are also able to provide and image which
would help us to assess the results
We have attached 2 images, one of GR121-7 and GR121-8. Identical
sections were stained with both antibodies, and you can quite clearly
see the difference between the 2 images.

Read More
Answer

Thank you for taking the time to complete our questionnaire and provide the images.

The details you have kindly provided will provide us with vital information for our monitoring of product quality.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there very few tips to provide on this occasion to help improve the results. I can suggestthat regrettably youmayhavereceived a bad vial.

I can recommend you may like to consider trying the following options which may help provide more consistent results between batches:

1. I can recommend to try serum to block rather than casein. Changing blocking agents can sometimes help to improve results.

2. I can suggest to wash in PBS containing 0.2% Tween. The Tweenwill help to keep the cells permeabilised, and the antibody solubilised.

3. I can recommend toaliquot and store at -20oC or -80oC as directed on the datasheet. This will help to ensure the antibody remains stable and that we can continue to provide our guarantee.

Alternatively, or if these tips do not work, I am pleased to offer you a free of charge replacement or credit note in compensation.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Read More

1-10 of 22 Abreviews or Q&A

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