Key features and details
- Mouse monoclonal [MAPK-YT] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Saccharomyces cerevisiae, Drosophila melanogaster
- Isotype: IgG1
产品名称Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187)抗体[MAPK-YT]
参阅全部 Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 一抗
描述小鼠单克隆抗体[MAPK-YT] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
经测试应用适用于: ICC/IF, WBmore details
种属反应性与反应: Rat, Saccharomyces cerevisiae, Drosophila melanogaster
预测可用于: Xenopus laevis, Zebrafish
表位The epitope recognized by ab50011 is located in the region of phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase (Thr185 and Tyr187 in ERK-2).
- Rat brain extract, Drosophila wing imaginal disc.
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot is from ascites and is still in stock as of 25th October 2016- GR185138. Lot numbers higher than GR185138 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.097% Sodium azide
Concentration information loading...
纯化说明Purified from tissue culture supernatant.
Lane 1 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011) at 1/10000 dilution
Lane 2 : no primary antibody
All lanes : Extract of Rat1 cells activated with 100µM
All lanes : Goat Anti-Mouse IgG, AlkPhos
Predicted band size: 44 kDa
Observed band size: 42,44 kDa why is the actual band size different from the predicted?
In situ immunostaining of the activated form of MAP kinase with ab50011 (green) in Drosophila wing imaginal disc (500 micrometers long).
ICC/IF of NRK cells labeling the activated form of MAP kinase with ab50011 (1/1000 dilution). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue).
ab50011 被引用在 120 文献中.
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- Tao J et al. Sfrp1 protects against acute myocardial ischemia (AMI) injury in aged mice by inhibiting the Wnt/ß-catenin signaling pathway. J Cardiothorac Surg 16:12 (2021). PubMed: 33468190
- Zhang C et al. Anti-Inflammatory Effect Fraction of Bletilla striata and Its Protective Effect on LPS-Induced Acute Lung Injury. Mediators Inflamm 2021:6684120 (2021). PubMed: 33776576