Anti-ERK1 (phospho Y204)抗体(ab131438)
Key features and details
- Rabbit polyclonal to ERK1 (phospho Y204)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ERK1 (phospho Y204)抗体
参阅全部 ERK1 一抗 -
描述
兔多克隆抗体to ERK1 (phospho Y204) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human ERK1 aa 202-206 (phospho Y204) conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:T-E-Y(p)-V-A
Database link: P27361 -
阳性对照
- 293 cell extract untreated or treated with UV; HeLa cells; Human breast carcinoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab131438 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131438于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 43 kDa.
|
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| IHC-P |
1/50 - 1/100.
|
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| ICC/IF |
1/100 - 1/200.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 43 kDa. |
|
IHC-P
1/50 - 1/100. |
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ICC/IF
1/100 - 1/200. |
靶标
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功能
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204. - Information by UniProt
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数据库链接
- Entrez Gene: 5595 Human
- Omim: 601795 Human
- SwissProt: P27361 Human
- Unigene: 861 Human
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别名
- ERK 1 antibody
- ERK-1 antibody
- ERK1 antibody
see all
图片
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Western blot - Anti-ERK1 (phospho Y204) antibody (ab131438)All lanes : Anti-ERK1 (phospho Y204) antibody (ab131438) at 1/500 dilution
Lane 1 : 293 cell extract untreated
Lane 2 : 293 cell extract treated with UV
Predicted band size: 43 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho Y204) antibody (ab131438)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ERK1 (phospho Y204) with ab131438 at 1/50 dilution. Right panel was preincubated with blocking peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-ERK1 (phospho Y204) antibody (ab131438)Immunofluorescent analysis of methanol-fixed HeLa cells labelling ERK1 (phospho Y204) with ab131438 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (27)
ab131438 被引用在 27 文献中.
- Xu W et al. PIK3CB promotes oesophageal cancer proliferation through the PI3K/AKT/mTOR signalling axis. Cell Biol Int 46:1399-1408 (2022). PubMed: 35842767
- Li L et al. Exploration of the Molecular Mechanism of Danzhi Xiaoyao Powder in Endometrial Cancer through Network Pharmacology. Evid Based Complement Alternat Med 2022:8330926 (2022). PubMed: 35774749
- Wang L et al. MiR-543 Inhibits the Migration and Epithelial-To-Mesenchymal Transition of TGF-ß-Treated Endometrial Stromal Cells via the MAPK and Wnt/ß-Catenin Signaling Pathways. Pathol Oncol Res 27:1609761 (2021). PubMed: 34257616
- Chen B et al. M1 Bone Marrow-Derived Macrophage-Derived Extracellular Vesicles Inhibit Angiogenesis and Myocardial Regeneration Following Myocardial Infarction via the MALAT1/MicroRNA-25-3p/CDC42 Axis. Oxid Med Cell Longev 2021:9959746 (2021). PubMed: 34745428
- Lin Y et al. USP22 promotes proliferation in renal cell carcinoma by stabilizing survivin. Oncol Lett 20:246 (2020). PubMed: 32973959