AB278538

重组Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187)抗体[ERK12T202Y204-A11]

Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11]

20ul selling size
Recombinant
Lab Essentials
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(42 Publications)

Rabbit Recombinant Monoclonal ERK1 phospho T202 + Y204 antibody. Suitable for Flow Cyt, WB and reacts with Mouse, Human samples. Cited in 42 publications. Immunogen corresponding to Synthetic Peptide within Human MAPK1.

查看别名

ERK1, PRKM3, MAPK3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, p44-ERK1, ERK-1, p44-MAPK

5 Images

Flow Cytometry - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11] (AB278538)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11] (AB278538)

Flow cytometric analysis of Jurkat cells, secondary antibody only negative control (blue), or treated with U0126 (red) or with PMA (green) using Phospho-Erk1/2 (T202/Y204) antibody ERK12T202Y204-A11 (Abwiz Cat. #1111) or Company C antibody at 0.43 µg/mL (manufacturer's recommended concentration).

Flow Cyt

Supplier Data

Flow Cytometry - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11] (AB278538)

ab278538 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of ab278538.

Supplier Data

Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11] (AB278538)

Western blot analysis of Ramos cells treated with U0126 or treated with TPA using 0.05 μg/mL of ab278538.

All lanes:

Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11] (ab278538) at 0.05 µg/mL

Lane 1:

Ramos (treated with U0126) cell extract

Lane 2:

Ramos (treated with TPA) cell extract

false

Supplier Data

Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11] (AB278538)

All lanes:

Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11] (ab278538) at 0.1 µg/mL

Lane 1:

Ramos cell extract untreated

Lane 2:

Ramos cell extract treated with PMA

Lane 3:

Ramos cell extract treated with U0126

Lane 4:

Ramos cell extract treated with U0126 followed by treatment with PMA

false

Supplier Data

Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11] (AB278538)

All lanes:

Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11] (ab278538) at 0.1 µg/mL

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen)(treated with TPA) cell extract .

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (treated with U0126) cell extract.

false

不同偶联物与剂型 (3)

660 APC

APC Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11]
519 FITC

FITC Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11]
578 PE

PE Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

ERK12T202Y204-A11

亚型

IgG

轻链类型

kappa

不含载体蛋白

反应种属

Mouse, Human

应用

WB, Flow Cyt

applications

免疫原

Synthetic Peptide within Human MAPK1. The exact immunogen used to generate this antibody is proprietary information.

P28482

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.05-0.1 µg/mL", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A/G

存储溶液

Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ERK1 and ERK2 also known as p44 and p42 MAPK respectively are important proteins in the MAP kinase signaling pathway. They are expressed in various tissues with significant presence in the brain lungs and skin. ERK1 has a molecular weight of approximately 44 kDa while ERK2 has a molecular weight of around 42 kDa. Both proteins become activated through phosphorylation which is essential for their function in cellular processes.

Biological function summary

ERK1 and ERK2 serve as key players in cellular growth differentiation and survival. They form part of a complex cascade where they transduce signals from the cell membrane to the nucleus after activation by phosphorylation. This phosphorylation enables them to modify various downstream targets involved in regulating gene expression and cellular response to external stimuli.

Pathways

ERK1 and ERK2 are critical components of the MAPK/ERK pathway and the Ras-Raf-MEK-ERK signaling cascade. These pathways regulate a multitude of cellular activities including proliferation and differentiation. In the MAPK/ERK pathway proteins like Ras and Raf serve as upstream activators of ERK1 and ERK2. Both ERK1 and ERK2 also interact with other signaling proteins such as MEK1/2 which directly phosphorylates and activates them.

Dysregulation of ERK1 and ERK2 is associated with various pathologies including cancer and neurodegenerative diseases. Abnormal activation of these proteins often leads to uncontrolled cell proliferation contributing to oncogenesis. For instance mutations in proteins like Ras which regulate ERK1 and ERK2 can result in continuous activation and lead to tumor formation. Furthermore altered ERK1 and ERK2 signaling is linked to neurodegeneration impacting neuronal survival and function.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed : 34497368). MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DEPTOR, FRS2 or GRB10) (PubMed : 35216969). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Phosphorylates GJA1 at 'Ser-279' and 'Ser-282' resulting in an increase in GJA1 ubiquitination and ultimately lysosomal degradation (By similarity).

See full target information MAPK3 phospho T202 + Y204

其他靶点

MAPK1 phospho T185 + Y187

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文献 (42)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 16:1642916 PubMed41040861

2025

Enhancing post-operative hypothyroidism treatment: rat thyroid autotransplantation into a pre-vascularized, retrievable cell pouch™ device.

Applications

Unspecified application

Species

Unspecified reactive species

Arash Memarnejadian,Pardis Pakshir,Madison Tomlinson,Farideh Berjisian,Ligia Dortas Maffei,Ben Muirhead,Jonathan Mofford,Babak Ataei Mehr,Vijay Parikh,Frank R Shannon,Pericles Calias,Philip M Toleikis

Scientific reports 15:29741 PubMed40804251

2025

HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2-Dectin-1/ SYK mediated ROS and inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Liu,Yingqian Zhang,Huifang Wu,Jingli Zhang,Yingxue Wang,Huifeng Zhang

Medical oncology (Northwood, London, England) 42:242 PubMed40483367

2025

CEBPB regulates ERK1/2 activity through SOS1 and contributes to ovarian cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahong Tan,Daoqi Wang,Aiqing Tu,Qin Xu,Li Zhuan,Xiaodie Wu,Lin Zhao,Wei Dong,Jie Zhang,Yun Feng

Journal of cellular and molecular medicine 29:e70609 PubMed40394754

2025

Erk1/2 Orchestrates SSPH I-Induced Oxidative Stress, Mitochondrial Dysfunction and Ferroptosis in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuewen Sun,Ying Zhou,Dan Huang,Zhiguang Zhao,Qingrui Shao,Jianzhe Li,Xiaofang Zhao,Xudong Liu

Journal of translational medicine 23:374 PubMed40140939

2025

Leveraging AAV1-Rac1T17N to prevent experimental proliferative vitreoretinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Duo Li,Minli Linghu,Jisen Tang,Gukun Yang,Chuanwu Li,Hang Yao,Hetian Lei,Yikeng Huang,Xionggao Huang

Cell reports. Medicine 6:102000 PubMed40056904

2025

LTA4H improves the tumor microenvironment and prevents HCC progression via targeting the HNRNPA1/LTBP1/TGF-β axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Yang,Xinyao Qiu,Yingcheng Yang,Jing Wu,Shan Wang,Bo Zheng,Jianmin Wu,Tao Zhou,Yangqianwen Zhang,Mixue Bai,Shuowu Liu,Zihan Zhao,Yani Zhang,Yixian Wang,Jinxia Bao,Mengye Wu,Dongdong Xue,Meiyu Bao,Ji Hu,Siyun Shen,Hongyang Wang,Lei Chen

Cell communication and signaling : CCS 23:72 PubMed39930412

2025

Transcription factor XBP1s promotes endometritis-induced epithelial-mesenchymal transition by targeting MAP3K2, a key gene in the MAPK/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kangkang Gao,Mengqi Si,Xinxi Qin,Beibei Zhang,Zongjie Wang,Pengfei Lin,Huatao Chen,Aihua Wang,Yaping Jin

Oncology research 33:199-212 PubMed39735670

2024

Lenalidomide regulates the CCL21/CCR7/ERK1/2 axis to inhibit migration and proliferation in diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Yang,Bin Tang,Dan Xu,Wenxiu Yang

Translational cancer research 13:6347-6363 PubMed39697757

2024

gene expression in melanoma tissues and its effects on the malignant biological functions of melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaojiao Qu,Xianfeng Cheng,Mingyan Liu,Qiang Zhang

BMC cancer 24:1189 PubMed39333927

2024

RPS6KA1 is a histone acetylation-related oncoprotein in acute myeloid leukemia which is targeted by afzelin.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Guo,Guinian Huang,Dafa Qiu,Huiqing He,Xiaomin Niu,Ziwen Guo,Yongbin Ye

View all publications

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重组Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187)抗体[ERK12T202Y204-A11]