Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187)抗体[SP327] (ab223500)
![Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)](https://www.abcam.cn/ps/products/223/ab223500/Images/ab223500-283000-anti-erk1-erk2-phospho-t202-y204-antibody-sp327-western-blot.jpg "Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)")
Key features and details
- Rabbit monoclonal [SP327] to ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187)
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187)抗体[SP327] -
描述
兔单克隆抗体[SP327] to ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: A431 + EGF cell lysates. IHC-P: Human breast ductal carcinoma, bladder transitional cell carcinoma and breast tissues. Flow Cyt: A431 + EGF cells.
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
SP327 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223500于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
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| WB |
1/400. Predicted molecular weight: 42,44 kDa.
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| IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
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Flow Cyt (Intra)
1/100. |
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WB
1/400. Predicted molecular weight: 42,44 kDa. |
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IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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细胞定位
ERK2: Nucleus. -
数据库链接
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Omim: 176948 Human
- Omim: 601795 Human
- SwissProt: P27361 Human
- SwissProt: P28482 Human
- Unigene: 431850 Human
- Unigene: 861 Human
图片
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Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)All lanes : Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500) at 1/400 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) + EGF cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Predicted band size: 42,44 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)](https://www.abcam.cn/ps/products/223/ab223500/Images/ab223500-282997-anti-erk1-erk2-phospho-t202-y204-antibody-sp327-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)Paraffin-embedded human breast ductal carcinoma tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.
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![Flow Cytometry (Intracellular) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)](https://www.abcam.cn/ps/products/223/ab223500/Images/ab223500-282999-anti-erk1-erk2-phospho-t202-y204-antibody-sp327-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)Flow cytometric analysis of ab223500 in A431 (human epidermoid carcinoma cell line) cells + EGF (green) at 1/100 dilution compared to negative control of rabbit IgG (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)](https://www.abcam.cn/ps/products/223/ab223500/Images/ab223500-282996-anti-erk1-erk2-phospho-t202-y204-antibody-sp327-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)Paraffin-embedded human breast tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)](https://www.abcam.cn/ps/products/223/ab223500/Images/ab223500-282995-anti-erk1-erk2-phospho-t202-y204-antibody-sp327-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)Paraffin-embedded human bladder transitional cell carcinoma tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (56)
ab223500 被引用在 56 文献中.
- Cifuentes SJ & Domenech M Heparin-collagen I bilayers stimulate FAK/ERK½ signaling via α2β1 integrin to support the growth and anti-inflammatory potency of mesenchymal stromal cells. J Biomed Mater Res A 112:65-81 (2024). PubMed: 37723658
- Gan Q et al. AC010883.5 promotes cell proliferation, invasion, migration, and epithelial-to-mesenchymal transition in cervical cancer by modulating the MAPK signaling pathway. BMC Cancer 23:364 (2023). PubMed: 37081411
- Deng Y et al. STIM1-regulated exosomal EBV-LMP1 empowers endothelial cells with an aggressive phenotype by activating the Akt/ERK pathway in nasopharyngeal carcinoma. Cell Oncol (Dordr) 46:987-1000 (2023). PubMed: 36917356
- Chu TY et al. Insulin-like growth factor (IGF) and hepatocyte growth factor (HGF) in follicular fluid cooperatively promote the oncogenesis of high-grade serous carcinoma from fallopian tube epithelial cells: Dissection of the molecular effects. Mol Carcinog 62:1417-1427 (2023). PubMed: 37265438
- Liu T et al. Sprouty-related proteins with EVH1 domain (SPRED2) prevents high-glucose induced endothelial-mesenchymal transition and endothelial injury by suppressing MAPK activation. Bioengineered 13:13882-13892 (2022). PubMed: 35707829