Name: Anti-ERK1 + ERK2 antibody [EPR17526]
Brand: RabMAb
SKU: ab184699
Rating: 5 (1 reviews)

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Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17526] to ERK1 + ERK2
Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB
Knockout validated
Reacts with: Mouse, Rat, Human

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP PE

产品名称

Anti-ERK1 + ERK2抗体[EPR17526]
参阅全部 ERK1 + ERK2 一抗
描述

兔单克隆抗体[EPR17526] to ERK1 + ERK2
宿主

Rabbit
经测试应用

适用于: Flow Cyt (Intra), IP, ICC/IF, WBmore details
种属反应性

与反应: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: Human ERK1 full length recombinant protein; Human ERK2 full length recombinant protein; A431, Jurkat, HeLa, HepG2, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain, heart, kidney and spleen lysates. ICC/IF: HeLa cells. Flow Cyt (intra): A431 cells. IP. PC-12 whole cell extract.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR17526
同种型

IgG
研究领域
- Cancer
- Cell Death
- Apoptosis
- Other

The Abpromise guarantee

Abpromise™承诺保证使用ab184699于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Flow Cyt (Intra)		1/440. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP		1/70.
ICC/IF	(1)	1/250.
WB		1/10000. Detects a band of approximately 44, 42 kDa (predicted molecular weight: 43, 41 kDa).

说明
Flow Cyt (Intra) 1/440. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/70.
ICC/IF 1/250.
WB 1/10000. Detects a band of approximately 44, 42 kDa (predicted molecular weight: 43, 41 kDa).

功能

Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
序列相似性

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
结构域

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
翻译后修饰

Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
细胞定位

Nucleus.
Target information above from: UniProt accession P28482 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- Entrez Gene: 116590 Rat
- Entrez Gene: 50689 Rat
- Omim: 176948 Human
- Omim: 601795 Human
- SwissProt: P27361 Human
- SwissProt: P28482 Human
- SwissProt: P63085 Mouse
- SwissProt: Q63844 Mouse
- SwissProt: P21708 Rat
- SwissProt: P63086 Rat
- Unigene: 431850 Human
- Unigene: 196581 Mouse
- Unigene: 34914 Rat
see all
形式

Mainly expressed in the cytoplasm and only localizes to the nucleus with treatment.
别名
- ERK 1 antibody
- ERK 2 antibody
- ERK-1 antibody
- ERK-2 antibody
- ERK1 antibody
- erk1/2 antibody
- ERK2 antibody
- ERT1 antibody
- ERT2 antibody
- Extracellular signal regulated kinase 1 antibody
- Extracellular signal-regulated kinase 1 antibody
- Extracellular signal-regulated kinase 2 antibody
- HS44KDAP antibody
- HUMKER1A antibody
- Insulin-stimulated MAP2 kinase antibody
- MAP kinase 1 antibody
- MAP kinase 2 antibody
- MAP kinase 3 antibody
- MAP kinase isoform p42 antibody
- MAP kinase isoform p44 antibody
- MAPK 1 antibody
- MAPK 2 antibody
- MAPK 3 antibody
- Mapk1 antibody
- MAPK2 antibody
- MAPK3 antibody
- Microtubule-associated protein 2 kinase antibody
- Mitogen-activated protein kinase 1 antibody
- Mitogen-activated protein kinase 2 antibody
- Mitogen-activated protein kinase 3 antibody
- MK01_HUMAN antibody
- p38 antibody
- p40 antibody
- p41 antibody
- p41mapk antibody
- p42-MAPK antibody
- P42MAPK antibody
- p44-ERK1 antibody
- p44-MAPK antibody
- p44ERK1 antibody
- p44MAPK antibody
- PRKM 2 antibody
- PRKM1 antibody
- PRKM2 antibody
- PRKM3 antibody
- protein tyrosine kinase ERK2 antibody
see all

Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)

All lanes : Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution

Lane 1 : Mouse brain lysates
Lane 2 : Mouse heart lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse spleen lysates
Lane 5 : Rat brain lysates
Lane 6 : Rat heart lysates
Lane 7 : Rat kidney lysates
Lane 8 : Rat spleen lysates
Lane 9 : C6 (Rat glial tumor cells) whole cell lysates
Lane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 43, 41 kDa
Observed band size: 44, 42 kDa why is the actual band size different from the predicted?

44kDa band represents ERK1. 42kDa band represents ERK2.
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)

All lanes : Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MAPK1 knockout HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 43, 41 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?

Lanes 1-2: Merged signal (red and green). Green - ab184699 observed at 44 kDa. Red - loading control ab8245 observed at 37 kDa.

ab184699 Anti-ERK1 + ERK2 antibody [EPR17526] was shown to specifically react with ERK2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265052 (knockout cell lysate ab257525) was used. Wild-type and ERK2 knockout samples were subjected to SDS-PAGE. ab184699 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)This image is courtesy of an Abreview submitted by Kirk Mcmanus.

Ab184699 staining ERK1 + ERK2 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. A Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150081) was used as the secondary antibody.
See Abreview
Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)

Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution + Recombinant Human ERK1 protein (ab43623) (ab43623) at 0.01 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 43, 41 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?

Recombinant full length ERK1 protein (ab43623) contains aa1-379.
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ERK1 + ERK2 with ab184699 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing both nuclear and cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab184699 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)

Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution + Recombinant Human ERK2 protein (ab43625) at 0.01 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 43, 41 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?

Recombinant full length ERK2 protein (ab43625) contains aa1-360.
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)

All lanes : Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/50000 dilution

Lane 1 : A431 (Human epidermoid carcinoma) whole cell lysates
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 4 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysates
Lane 5 : Human fetal brain lysates
Lane 6 : Human fetal heart lysates
Lane 7 : Human fetal kidney lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 43, 41 kDa
Observed band size: 42,44 kDa why is the actual band size different from the predicted?

44kDa band represents ERK1. 42kDa band represents ERK2.
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)

All lanes : Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution

Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal heart lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 43, 41 kDa
Observed band size: 42,44 kDa why is the actual band size different from the predicted?

44kDa band represents ERK1. 42kDa band represents ERK2.
Blocking/Dilution buffer: 5% NFDM/TBST.
Flow Cytometry (Intracellular) - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)

Intracellular flow cytometric analysis of A431 (Human epidermoid carcinoma) cells labeling ERK1 + ERK2 with ab184699 at 1/440 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)

ERK1 + ERK2 were immunoprecipitated from 1mg of PC-12 (Rat adrenal gland pheochromocytoma) whole cell extract with ab184699 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab184699 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: PC-12 whole cell extract. Lane 2: PBS instead of PC-12 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
44kDa band represents ERK1. 42kDa band represents ERK2.
Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)

Flow cytometry protocols
Immunoprecipitation protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

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Datasheet download

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发表研究结果有使用 ab184699？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab184699 被引用在 121 文献中.

Shi L et al. Spaceflight and simulated microgravity suppresses macrophage development via altered RAS/ERK/NF?B and metabolic pathways. Cell Mol Immunol 18:1489-1502 (2021). PubMed: 31900461
He XL et al. Solid lipid nanoparticles loading with curcumin and dexanabinol to treat major depressive disorder. Neural Regen Res 16:537-542 (2021). PubMed: 32985484
Wang X et al. C-C chemokine receptor 5 signaling contributes to cardiac remodeling and dysfunction under pressure overload. Mol Med Rep 23:N/A (2021). PubMed: 33200795
Fei J et al. Inhibition of SNCG suppresses the proliferation of lung cancer cells induced by high glucose. Mol Med Rep 23:N/A (2021). PubMed: 33313952
Hou TY et al. JNK and p38 Inhibitors Prevent Transforming Growth Factor-ß1-Induced Myofibroblast Transdifferentiation in Human Graves' Orbital Fibroblasts. Int J Mol Sci 22:N/A (2021). PubMed: 33799469

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Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Permeabilization

Yes - 0.5% Triton X-100

Specification

HeLa

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

提交于 Jul 26 2017

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Key features and details

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概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

KO cell lines

KO cell lysates

Related Products

应用

The Abpromise guarantee

靶标

功能

序列相似性

结构域

翻译后修饰

细胞定位

数据库链接

形式

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

文献 (121)

客户评价及客户问答

Immunocytochemistry/ Immunofluorescence abreview for Anti-ERK1 + ERK2 antibody [EPR17526]