重组Anti-ERGIC3抗体[EPR8141] (ab129179)
![Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179)](https://www.abcam.cn/ps/products/129/ab129179/Images/ab129179-410223-anti-ergic3-antibody-epr8141-western-blot-human-knockout-lysate.jpg "Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8141] to ERGIC3
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ERGIC3抗体[EPR8141]
参阅全部 ERGIC3 一抗 -
描述
兔单克隆抗体[EPR8141] to ERGIC3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human ERGIC3 aa 100-200. The exact sequence is proprietary.
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阳性对照
- WB: HepG2, MCF7, 293T, K562 and HeLa cell lysates. IHC-P: Human brain tissue. ICC: HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 5.36 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8141 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129179于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF | (1) |
1/250 - 1/500.
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| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
ICC/IF
1/250 - 1/500. |
靶标
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功能
Possible role in transport between endoplasmic reticulum and Golgi. -
序列相似性
Belongs to the ERGIC family. -
细胞定位
Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus > cis-Golgi network membrane. Endoplasmic reticulum membrane. Cycles between the endoplasmic reticulum and the Golgi. - Information by UniProt
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数据库链接
- Entrez Gene: 51614 Human
- Entrez Gene: 66366 Mouse
- Entrez Gene: 296306 Rat
- SwissProt: Q9Y282 Human
- SwissProt: Q9CQE7 Mouse
- Unigene: 472558 Human
- Unigene: 141276 Mouse
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别名
- 2310015B14Rik antibody
- AV318804 antibody
- C20orf47 antibody
see all
图片
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Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179)All lanes : Anti-ERGIC3 antibody [EPR8141] (ab129179) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ERGIC3 knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : MCF-7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab129179 observed at 50 kDa. Red - loading control ab8245 observed at 36 kDa.
ab129179 Anti-ERGIC3 antibody [EPR8141] was shown to specifically react with ERGIC3 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266489 (knockout cell lysate ab257941) was used. Wild-type and ERGIC3 knockout samples were subjected to SDS-PAGE. ab129179 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC3 antibody [EPR8141] (ab129179)](https://www.abcam.cn/ps/products/129/ab129179/Images/ab129179-143205-anti-ergi3-antibody-epr8141-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC3 antibody [EPR8141] (ab129179)ab129179, at 1/100, staining ERGIC3 in paraffin embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-ERGIC3 antibody [EPR8141] (ab129179)](https://www.abcam.cn/ps/products/129/ab129179/Images/ab129179-143204-anti-ergi3-antibody-epr8141-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ERGIC3 antibody [EPR8141] (ab129179)ab129179, at 1/250, staining ERGIC3 in HeLa cells by Immunofluorescence.
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![Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179)](https://www.abcam.cn/ps/products/129/ab129179/Images/ab129179-143206-anti-ergi3-antibody-epr8141-western-blot.jpg)
Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179)All lanes : Anti-ERGIC3 antibody [EPR8141] (ab129179) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : K562 cell lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
![OI-RD Scanning - Anti-ERGIC3 antibody [EPR8141] (ab129179)](https://www.abcam.cn/ps/products/129/ab129179/Images/ab129179-143203-anti-ergi3-antibody-epr8141.jpg)
OI-RD Scanning - Anti-ERGIC3 antibody [EPR8141] (ab129179) -
![Anti-ERGIC3 antibody [EPR8141] (ab129179)](https://www.abcam.cn/ps/products/129/ab129179/Images/ab129179-6-benefits-of-recombinant-antibodies.png)
Anti-ERGIC3 antibody [EPR8141] (ab129179)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab129179 被引用在 3 文献中.
- Guan L et al. ERGIC2 and ERGIC3 regulate the ER-to-Golgi transport of gap junction proteins in metazoans. Traffic 23:140-157 (2022). PubMed: 34994051
- Hirano M et al. A highly photostable and bright green fluorescent protein. Nat Biotechnol 40:1132-1142 (2022). PubMed: 35468954
- Yoo W et al. The E3 ubiquitin ligase MARCH2 regulates ERGIC3-dependent trafficking of secretory proteins. J Biol Chem 294:10900-10912 (2019). PubMed: 31142615