Anti-ERGIC1抗体(ab121574)
Key features and details
- Rabbit polyclonal to ERGIC1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ERGIC1抗体
参阅全部 ERGIC1 一抗 -
描述
兔多克隆抗体to ERGIC1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: VVNELYVDDP DKDSGGKIDV SLNISLPNLH CELVGLDIQD EMGRHEVGHI DNSMKIPLNN GAGCRFEGQF SINK, corresponding to internal sequence amino acids 52-125 of Human ERGIC1
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阳性对照
- WB: Human RT-4, U-251 MG and Human tonsil cell lysates IHC: Human liver, prostate, cerebral cortex and testis tissue ICC/IF: Human cell line U-251
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121574于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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功能
Possible role in transport between endoplasmic reticulum and Golgi. -
序列相似性
Belongs to the ERGIC family. -
翻译后修饰
N-glycosylated. -
细胞定位
Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Cycles between the endoplasmic reticulum and the Golgi. - Information by UniProt
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数据库链接
- Entrez Gene: 57222 Human
- SwissProt: Q969X5 Human
- Unigene: 509163 Human
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别名
- endoplasmic reticulum-golgi intermediate compartment 32 kDa protein antibody
- Endoplasmic reticulum-Golgi intermediate compartment protein 1 antibody
- ER-Golgi intermediate compartment 32 kDa protein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (ab121574)
ab121574, at a 1/25 dilution, staining ERGIC1 in paraffin-embedded Human liver tissue by immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (ab121574)ab121574, at a 1/25 dilution, staining ERGIC1 in paraffin-embedded Human prostate tissue by immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (ab121574)ab121574, at a 1/25 dilution, staining ERGIC1 in paraffin-embedded Human cerebral cortex tissue by immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (ab121574)ab121574, at a 1/25 dilution, staining ERGIC1 in paraffin-embedded Human testis tissue by immunohistochemistry.
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Western blot - Anti-ERGIC1 antibody (ab121574)All lanes : Anti-ERGIC1 antibody (ab121574) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique. -
Immunocytochemistry/ Immunofluorescence - Anti-ERGIC1 antibody (ab121574)Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121574 1-4 µg/ml. Cells treated with PFA/Triton X-100.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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