Anti-eRF3/GSPT1抗体(ab49878)
Key features and details
- Rabbit polyclonal to eRF3/GSPT1
- Suitable for: IP, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-eRF3/GSPT1抗体
参阅全部 eRF3/GSPT1 一抗 -
描述
兔多克隆抗体to eRF3/GSPT1 -
宿主
Rabbit -
经测试应用
适用于: IP, ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human eRF3/GSPT1 aa 190-204 (internal sequence) conjugated to keyhole limpet haemocyanin (Cysteine residue).
Sequence:FETGFEKGGQTREHA
Database link: P15170 -
阳性对照
- WB: HeLa whole cell lysate (ab150035).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab49878于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
Paraformadehyde fixed cells |
|
| WB | (2) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
IP
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. Paraformadehyde fixed cells |
|
WB
Use at an assay dependent concentration. |
靶标
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功能
Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. -
序列相似性
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. ERF3 subfamily.
Contains 1 tr-type G (guanine nucleotide-binding) domain. - Information by UniProt
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数据库链接
- Entrez Gene: 2935 Human
- Omim: 139259 Human
- SwissProt: P15170 Human
- Unigene: 528780 Human
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别名
- 551G9.2 antibody
- Anti Eukaryotic Release Factor 3a antibody
- Anti G1 to S phase transition 1 antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab49878 被引用在 17 文献中.
- Hanzl A et al. Functional E3 ligase hotspots and resistance mechanisms to small-molecule degraders. Nat Chem Biol 19:323-333 (2023). PubMed: 36329119
- Lee RE et al. Small-molecule eRF3a degraders rescue CFTR nonsense mutations by promoting premature termination codon readthrough. J Clin Invest 132:N/A (2022). PubMed: 35900863
- Powell CE et al. Selective degradation-inducing probes for studying cereblon (CRBN) biology. RSC Med Chem 12:1381-1390 (2021). PubMed: 34458741
- Shimizu N et al. PLZF and its fusion proteins are pomalidomide-dependent CRBN neosubstrates. Commun Biol 4:1277 (2021). PubMed: 34764413
- Tateno S et al. Genome-wide screening reveals a role for subcellular localization of CRBN in the anti-myeloma activity of pomalidomide. Sci Rep 10:4012 (2020). PubMed: 32132601