AB137033

重组Anti-ERCC8抗体[EPR9237]

Anti-ERCC8 antibody [EPR9237]

20ul selling size
RabMAb
Recombinant
KO Validated
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(16 Publications)

Rabbit Recombinant Monoclonal ERCC8 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 16 publications.

查看别名

CKN1, CSA, ERCC8, DNA excision repair protein ERCC-8, Cockayne syndrome WD repeat protein CSA

3 Images

Lab

Immunoprecipitation - Anti-ERCC8 antibody [EPR9237] (AB137033)

Purified ab137033 at 1/80 dilution (2μg) immunoprecipitating ERCC8 in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab137033 + HeLa whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab137033 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 44 kDa

All lanes:

Immunoprecipitation - Anti-ERCC8 antibody [EPR9237] (ab137033)

Predicted band size: 44 kDa

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Unknown

Western blot - Anti-ERCC8 antibody [EPR9237] (AB137033)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : ERCC8 knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : Molt-4 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab137033 observed at 44 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab137033 was shown to recognize ERCC8 when ERCC8 knockout samples were used, along with additional cross-reactive bands. Wild-type and ERCC8 knockout samples were subjected to SDS-PAGE. ab137033 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively, and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-ERCC8 antibody [EPR9237] (ab137033)

Predicted band size: 44 kDa

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Unknown

Western blot - Anti-ERCC8 antibody [EPR9237] (AB137033)

All lanes:

Western blot - Anti-ERCC8 antibody [EPR9237] (ab137033) at 1/1000 dilution

Lane 1:

MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate at 15 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Lane 3:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/5000 dilution

Predicted band size: 44 kDa

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不同偶联物与剂型 (1)

Carrier free

Anti-ERCC8 antibody [EPR9237] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR9237

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ERCC8 also known as CSA protein plays an essential role in the repair of transcription-coupled nucleotide excision repair (TC-NER) pathways. This protein has a mass of about 44 kDa. Expression of ERCC8 is found in several tissues notably in the skin liver and nervous system. It helps in recognizing and repairing DNA damage that occurs during transcription.

Biological function summary

ERCC8 forms a part of the CSA complex which includes other proteins involved in the TC-NER pathway. The CSA complex targets the repair machinery to the site of transcription-blocking lesions. Identifying these lesions is critical to resuming normal transcription processes. The CSA protein therefore actively contributes to maintaining genomic stability by facilitating the correction of transcription-coupled DNA damage.

Pathways

ERCC8 participates directly in the nucleotide excision repair (NER) pathway specifically in its transcription-coupled repair subprocess. This pathway is integral to correcting helix-distorting DNA lesions that impede transcription. ERCC8 interacts closely with the Cockayne syndrome B (CSB) protein and RNA polymerase II during the repair process. This collaboration is important in modulating the restart of transcription post-repair.

Mutations in ERCC8 lead to Cockayne syndrome (CS) characterized by growth defects neurological dysfunction and photosensitivity. ERCC8 alterations are linked primarily to Cockayne syndrome group A. The protein is also involved in repair mechanisms tied to UV-sensitive syndrome. In both conditions faulty ERCC8 function can disrupt normal DNA repair processes leading to severe clinical manifestations associated with DNA repair failure. The role of CSA alongside CSB mutations further highlights its importance in disease phenotypes and the need for effective DNA repair.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair (TC-NER), a process during which RNA polymerase II-blocking lesions are rapidly removed from the transcribed strand of active genes (PubMed : 12732143, PubMed : 16751180, PubMed : 16964240, PubMed : 32142649, PubMed : 34526721, PubMed : 38316879, PubMed : 38600235, PubMed : 38600236). Following recruitment to lesion-stalled RNA polymerase II (Pol II), the CSA complex mediates ubiquitination of Pol II subunit POLR2A/RPB1 at 'Lys-1268', a critical TC-NER checkpoint, governing RNA Pol II stability and initiating DNA damage excision by TFIIH recruitment (PubMed : 12732143, PubMed : 16751180, PubMed : 16964240, PubMed : 32142649, PubMed : 32355176, PubMed : 34526721, PubMed : 38316879, PubMed : 38600235, PubMed : 38600236). The CSA complex also promotes the ubiquitination and subsequent proteasomal degradation of ERCC6/CSB in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair (PubMed : 16751180). Also plays a role in DNA double-strand breaks (DSSBs) repair by non-homologous end joining (NHEJ) (PubMed : 29545921).

See full target information ERCC8

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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation : PubMed40374849

2025

Cockayne syndrome mice reflect human kidney disease and are defective in de novo NAD biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Komal Pekhale,Vinod Tiwari,Mansoor Hussain,Christy C Bridges,Deborah L Croteau,Moshe Levi,Avi Z Rosenberg,Briana Santo,Xiaoping Yang,Tomasz Kulikowicz,Xiaoxin X Wang,Jong-Hyuk Lee,Vilhelm A Bohr

Nucleic acids research 53: PubMed40219966

2025

Poly-(ADP-ribose) serves as a scaffold for the methyltransferase METTL3/14 complex in the DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Gonzalez-Leal,Jin Cai,Bram A F J de Groot,Andreas Wegerer,Julia Preisser,Martijn S Luijsterburg,Andreas G Ladurner

Frontiers in oncology 14:1476100 PubMed39582530

2024

Characterization of the function and clinical value of ERCC family genes in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhimin Lu,Guoxin Hou

Nature communications 15:7089 PubMed39154022

2024

Coordination of transcription-coupled repair and repair-independent release of lesion-stalled RNA polymerase II.

Applications

Unspecified application

Species

Unspecified reactive species

Yongchang Zhu,Xiping Zhang,Meng Gao,Yanchao Huang,Yuanqing Tan,Avital Parnas,Sizhong Wu,Delin Zhan,Sheera Adar,Jinchuan Hu

Cell reports methods 4:100674 PubMed38176411

2024

Live-cell imaging of endogenous CSB-mScarletI as a sensitive marker for DNA-damage-induced transcription stress.

Applications

Unspecified application

Species

Unspecified reactive species

Di Zhou,Qing Yu,Roel C Janssens,Jurgen A Marteijn

Nucleic acids research 51:6238-6245 PubMed37144462

2023

Nucleotide excision repair in Human cell lines lacking both XPC and CSB proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Laura A Lindsey-Boltz,Yanyan Yang,Cansu Kose,Nazli Deger,Khagani Eynullazada,Hiroaki Kawara,Aziz Sancar

Cells 11: PubMed36231052

2022

A Novel Missense Mutation in Co-Segregates with Cerebellar Ataxia in a Consanguineous Pakistani Family.

Applications

Unspecified application

Species

Unspecified reactive species

Zeeshan Gauhar,Leon Tejwani,Uzma Abdullah,Sadia Saeed,Shagufta Shafique,Mazhar Badshah,Jungmin Choi,Weilai Dong,Carol Nelson-Williams,Richard P Lifton,Janghoo Lim,Ghazala K Raja

Nature communications 13:974 PubMed35190564

2022

Global and transcription-coupled repair of 8-oxoG is initiated by nucleotide excision repair proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Namrata Kumar,Arjan F Theil,Vera Roginskaya,Yasmin Ali,Michael Calderon,Simon C Watkins,Ryan P Barnes,Patricia L Opresko,Alex Pines,Hannes Lans,Wim Vermeulen,Bennett Van Houten

Cell 180:1228-1244.e24 PubMed32142649

2020

Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yuka Nakazawa,Yuichiro Hara,Yasuyoshi Oka,Okiru Komine,Diana van den Heuvel,Chaowan Guo,Yasukazu Daigaku,Mayu Isono,Yuxi He,Mayuko Shimada,Kana Kato,Nan Jia,Satoru Hashimoto,Yuko Kotani,Yuka Miyoshi,Miyako Tanaka,Akira Sobue,Norisato Mitsutake,Takayoshi Suganami,Akio Masuda,Kinji Ohno,Shinichiro Nakada,Tomoji Mashimo,Koji Yamanaka,Martijn S Luijsterburg,Tomoo Ogi

Nucleic acids research 48:2473-2485 PubMed31970402

2020

Cockayne syndrome group A and B proteins function in rRNA transcription through nucleolin regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Mustafa N Okur,Jong-Hyuk Lee,Wasif Osmani,Risako Kimura,Tyler G Demarest,Deborah L Croteau,Vilhelm A Bohr

View all publications

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重组Anti-ERCC8抗体[EPR9237]