重组Anti-ERCC1抗体[EPR7062] (ab129093)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7062] to ERCC1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ERCC1抗体[EPR7062]
参阅全部 ERCC1 一抗 -
描述
兔单克隆抗体[EPR7062] to ERCC1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment within Human ERCC1 aa 150-300. The exact sequence is proprietary.
Database link: P07992 -
阳性对照
- HeLa, SKBR-3, and 293T cell lysates, Human lung carcinoma tissue, HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7062 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129093于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
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IHC-P |
1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa). |
IHC-P
1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
靶标
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功能
Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. -
疾病相关
Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur. -
序列相似性
Belongs to the ERCC1/RAD10/SWI10 family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2067 Human
- Entrez Gene: 13870 Mouse
- Entrez Gene: 292673 Rat
- Omim: 126380 Human
- SwissProt: P07992 Human
- SwissProt: P07903 Mouse
- Unigene: 435981 Human
- Unigene: 280913 Mouse
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别名
- COFS 4 antibody
- COFS4 antibody
- DNA excision repair protein ERCC 1 antibody
see all
图片
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All lanes : Anti-ERCC1 antibody [EPR7062] (ab129093) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : SKBR-3 cell lysates
Lane 3 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [EPR7062] (ab129093)
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for ERCC1 with ab129093 (1/25 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab129093, at a 1/100 dilution, staining ERCC1 in HeLa cells by Immunofluorescence.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab129093 尚未被引用在任何文献中。