AB196869

Anti-ERCC1抗体

Name: ERCC1抗体 (ab196869)| Abcam中文官网
Brand: Abcam Limited
SKU: AB196869
Price: 4922 CNY
Availability: InStock

Anti-ERCC1 antibody

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(1 Publication)

Rabbit Polyclonal ERCC1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ERCC1.

查看别名

DNA excision repair protein ERCC-1, ERCC1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody (AB196869)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody (AB196869)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ERCC1 with ab196869 at 1/50.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody (AB196869)

Immunohistochemical analysis of paraffin-embedded Human kidney cancer tissue labeling ERCC1 with ab196869 at 1/50.

Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody (AB196869)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody (AB196869)

Immunofluorescent analysis of U2OS cells labeling ERCC1 with ab196869 at 1/50. Image at the bottom costained with DAPI.

Supplier Data

Western blot - Anti-ERCC1 antibody (AB196869)

All lanes:

Western blot - Anti-ERCC1 antibody (ab196869) at 1/500 dilution

Lane 1:

Raji cell line extract

Lane 2:

MCF7 cell line extract

Lane 3:

PC3 cell line extract

Lane 4:

HepG2 cell line extract

Lane 5:

HeLa cell line extract

Lane 6:

Mouse liver tissue extract

Lane 7:

Mouse brain tissue extract

Predicted band size: 33 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IHC-P, WB, ICC/IF

applications

免疫原

Recombinant Full Length Protein corresponding to Human ERCC1.

P07992

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ERCC1 also known as Excision Repair Cross-Complementation Group 1 is an essential protein in DNA repair mechanisms. Weighing approximately 36 kDa ERCC1 is expressed in a variety of tissues throughout an organism. It carries out its function by forming a heterodimer with XPF which is a structure-specific endonuclease playing a vital role in the nucleotide excision repair (NER) pathway that helps maintain genomic stability. The presence of an antibody like 8F1 can be helpful for detecting the ERCC1 protein expression in biological samples through techniques like ELISA.

Biological function summary

ERCC1 plays a significant role in the DNA damage repair process where it interacts with its partner protein XPF to excise damaged DNA. The ERCC1-XPF complex recognizes and cuts both single- and double-stranded DNA therefore enabling the removal of DNA lesions caused by UV radiation or chemical mutagens. This action prevents mutations by ensuring accurate DNA replication and transcription.

Pathways

The ERCC1 protein is primarily involved in the nucleotide excision repair (NER) and interstrand cross-link repair pathways. Within these pathways ERCC1 provides repair capacity to the cell maintaining the functionality of the DNA by partnering with proteins such as XPF and participating in cross-link repair mechanisms. By coordinating with proteins like XPA and RPA ERCC1 assists in identifying and binding the damaged DNA facilitating the recruitment of other repair proteins.

The ERCC1 protein is linked to conditions such as Xeroderma Pigmentosum and Fanconi Anemia largely due to its critical role in DNA repair. Malfunctions or deficiencies in ERCC1 or its partner proteins can lead to compromised DNA repair resulting in susceptibility to cancer and other genomic instability disorders. ERCC1's relationship with proteins that partake in cell cycle regulation further emphasizes its impact on disease progression linking it with potential therapeutic targets for addressing these genetic disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Isoform 1. Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.. Isoform 2. Not functional in the nucleotide excision repair pathway.. Isoform 3. Not functional in the nucleotide excision repair pathway.. Isoform 4. Not functional in the nucleotide excision repair pathway.

See full target information ERCC1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40548942

2025

The lincRNA Pantr1 is a FOXG1 target gene conferring site-specific chromatin binding of FOXG1.

Applications

Unspecified application

Species

Unspecified reactive species

Fabian Gather,Tudor Rauleac,Ipek Akol,Ganeshkumar Arumugam,Camila L Fullio,Teresa Müller,Dimitrios Kleidonas,Ruth Geiss-Friedlander,Andre Fischer,Andreas Vlachos,Rolf Backofen,Tanja Vogel

View all publications

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Anti-ERCC1抗体

Anti-ERCC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody (AB196869)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody (AB196869)

Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody (AB196869)

Western blot - Anti-ERCC1 antibody (AB196869)