AB2356

Anti-ERCC1 抗体 [8F1]

Name: ERCC1抗体[8F1] (ab2356)| Abcam中文官网
Brand: Abcam Limited
SKU: AB2356
Price: 5035 CNY
Availability: InStock

Anti-ERCC1 antibody [8F1]

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(28 Publications)

Mouse Monoclonal ERCC1 antibody. Suitable for IHC-P, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 28 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ERCC1.

查看别名

DNA excision repair protein ERCC-1, ERCC1

4 Images

Flow Cytometry - Anti-ERCC1 antibody [8F1] (AB2356)

Flow Cyt

Unknown

Flow Cytometry - Anti-ERCC1 antibody [8F1] (AB2356)

Overlay histogram showing HeLa cells stained with ab2356 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2356, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [8F1] (AB2356)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [8F1] (AB2356)

Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil tissue sections with ab2356 labelling ERCC1.

Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody [8F1] (AB2356)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody [8F1] (AB2356)

ICC/IF image of ab2356 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2356, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [8F1] (AB2356)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue with ab2356 labelling ERCC1.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

8F1

亚型

IgG2b

轻链类型

unknown

不含载体蛋白

反应种属

Human

应用

Flow Cyt, IHC-P, ICC/IF

applications

免疫原

Recombinant Full Length Protein corresponding to Human ERCC1.

P07992

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: 1% BSA

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ERCC1 also known as Excision Repair Cross-Complementation Group 1 is an essential protein in DNA repair mechanisms. Weighing approximately 36 kDa ERCC1 is expressed in a variety of tissues throughout an organism. It carries out its function by forming a heterodimer with XPF which is a structure-specific endonuclease playing a vital role in the nucleotide excision repair (NER) pathway that helps maintain genomic stability. The presence of an antibody like 8F1 can be helpful for detecting the ERCC1 protein expression in biological samples through techniques like ELISA.

Biological function summary

ERCC1 plays a significant role in the DNA damage repair process where it interacts with its partner protein XPF to excise damaged DNA. The ERCC1-XPF complex recognizes and cuts both single- and double-stranded DNA therefore enabling the removal of DNA lesions caused by UV radiation or chemical mutagens. This action prevents mutations by ensuring accurate DNA replication and transcription.

Pathways

The ERCC1 protein is primarily involved in the nucleotide excision repair (NER) and interstrand cross-link repair pathways. Within these pathways ERCC1 provides repair capacity to the cell maintaining the functionality of the DNA by partnering with proteins such as XPF and participating in cross-link repair mechanisms. By coordinating with proteins like XPA and RPA ERCC1 assists in identifying and binding the damaged DNA facilitating the recruitment of other repair proteins.

The ERCC1 protein is linked to conditions such as Xeroderma Pigmentosum and Fanconi Anemia largely due to its critical role in DNA repair. Malfunctions or deficiencies in ERCC1 or its partner proteins can lead to compromised DNA repair resulting in susceptibility to cancer and other genomic instability disorders. ERCC1's relationship with proteins that partake in cell cycle regulation further emphasizes its impact on disease progression linking it with potential therapeutic targets for addressing these genetic disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Isoform 1. Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.. Isoform 2. Not functional in the nucleotide excision repair pathway.. Isoform 3. Not functional in the nucleotide excision repair pathway.. Isoform 4. Not functional in the nucleotide excision repair pathway.

See full target information ERCC1

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文献 (28)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 8:e09643 PubMed35711974

2022

Association between TOP2A, RRM1, HER2, ERCC1 expression and response to chemotherapy in patients with non-muscle invasive bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhifei Liu,Liyong Xing,Yanfeng Zhu,Peng Shi,Gang Deng

BMC cancer 20:960 PubMed33023532

2020

TIMP-2 regulates proliferation, invasion and STAT3-mediated cancer stem cell-dependent chemoresistance in ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ruth M Escalona,Maree Bilandzic,Patrick Western,Elif Kadife,George Kannourakis,Jock K Findlay,Nuzhat Ahmed

Oncology letters 20:212 PubMed32963618

2020

ERCC overexpression associated with a poor response of cT4b colorectal cancer with FOLFOX-based neoadjuvant concurrent chemoradiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Yii Huang,Hsin-Hua Lee,Ching-Wen Huang,Chun-Ming Huang,Cheng-Jen Ma,Tzu-Chieh Yin,Hsiang-Lin Tsai,Chee-Yin Chai,Yi-Ting Chen,Jaw-Yuan Wang

Anticancer research 39:2483-2491 PubMed31092443

2019

An Exploratory Randomized Phase II Trial Comparing CDDP Plus S-1 With Bevacizumab and CDDP Plus Pemetrexed With Bevacizumab Against Patients With Advanced Non-squamous Non-small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kyoichi Kaira,Hisao Imai,Ryousuke Souma,Reiko Sakurai,Yosuke Miura,Noriaki Sunaga,Norimitsu Kasahara,Yusuke Tsukagoshi,Yasuhiko Koga,Shinsuke Kitahara,Mie Kotake,Koichi Minato,Ichiro Naruse,Yasutsugu Fukushima,Takeshi Hisada,Tamotsu Ishizuka

Pathology oncology research : POR 26:1105-1116 PubMed31077069

2019

Predictive Value of ERCC1, ERCC2, and XRCC Expression for Patients with Locally Advanced or Metastatic Gastric Cancer Treated with Neoadjuvant mFOLFOX-4 Chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yung-Sung Yeh,Yi-Ting Chen,Hsiang-Lin Tsai,Ching-Wen Huang,Cheng-Jen Ma,Wei-Chih Su,Chun-Ming Huang,Ming-Yii Huang,Huang-Ming Hu,Chien-Yu Lu,Jaw-Yuan Wang

EBioMedicine 35:204-221 PubMed30146342

2018

The miR 495-UBE2C-ABCG2/ERCC1 axis reverses cisplatin resistance by downregulating drug resistance genes in cisplatin-resistant non-small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwei Guo,Dan Jin,Yan Wu,Lijuan Yang,Jing Du,Kaikai Gong,Weiwei Chen,Juanjuan Dai,Shuang Miao,Sichuan Xi

Experimental and therapeutic medicine 16:1143-1148 PubMed30116365

2018

Effect of lanthanum chloride on tumor growth and apoptosis in human ovarian cancer cells and xenograft animal models.

Applications

Unspecified application

Species

Unspecified reactive species

Fen Wang,Yuanfang Zhu,Shanyu Fang,Shuya Li,Sisun Liu

The international journal of biochemistry & cell b 100:54-60 PubMed29768183

2018

NDRG1 disruption alleviates cisplatin/sodium glycididazole-induced DNA damage response and apoptosis in ERCC1-defective lung cancer cells.

Applications

Species

Unspecified reactive species

Lang He,Kang Liu,Xiaoshan Wang,Hong Chen,Jin Zhou,Xun Wu,Tao Liu,Yongxue Yang,Xuemei Yang,Dandan Cui,Guiqin Song,Jun Wang,Jianguo Lei

Molecular and clinical oncology 9:3-10 PubMed29977534

2018

Thymidylate synthase expression in primary colorectal cancer as a predictive marker for the response to 5-fluorouracil- and oxaliplatin-based preoperative chemotherapy for liver metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroshi Takeyama,Tomoko Wakasa,Keisuke Inoue,Kotaro Kitani,Masanori Tsujie,Takafumi Ogawa,Masao Yukawa,Yoshio Ohta,Masatoshi Inoue

Nature communications 9:259 PubMed29343685

2018

The protective role of DOT1L in UV-induced melanomagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhu,Shuyang Chen,Hongshen Wang,Chengqian Yin,Changpeng Han,Cong Peng,Zhaoqian Liu,Lixin Wan,Xiaoyang Zhang,Jie Zhang,Christine G Lian,Peilin Ma,Zhi-Xiang Xu,Sharon Prince,Tao Wang,Xiumei Gao,Yujiang Shi,Dali Liu,Min Liu,Wenyi Wei,Zhi Wei,Jingxuan Pan,Yongjun Wang,Zhenyu Xuan,Jay Hess,Nicholas K Hayward,Colin R Goding,Xiang Chen,Jun Zhou,Rutao Cui

View all publications

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Anti-ERCC1 抗体 [8F1]

Anti-ERCC1 antibody [8F1]

Flow Cytometry - Anti-ERCC1 antibody [8F1] (AB2356)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [8F1] (AB2356)

Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody [8F1] (AB2356)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [8F1] (AB2356)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

不含载体蛋白

反应种属

应用

免疫原