重组Anti-ErbB3 / HER3 (phospho Y1328)抗体[EPR5809] (ab133459)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5809] to ErbB3 / HER3 (phospho Y1328)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ErbB3 / HER3 (phospho Y1328)抗体[EPR5809]
参阅全部 ErbB3 / HER3 一抗 -
描述
兔单克隆抗体[EPR5809] to ErbB3 / HER3 (phospho Y1328) -
宿主
Rabbit -
特异性
This antibody only detects ErbB 3 phosphorylated at Tyrosine 1328.
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经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- T47-D cell lysate treated with Neuregulin.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.31 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR5809 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133459于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 148 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 148 kDa. |
靶标
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功能
Binds and is activated by neuregulins and NTAK. -
组织特异性
Epithelial tissues and brain. -
疾病相关
Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
发展阶段
Overexpressed in a subset of human mammary tumors. -
结构域
The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. -
翻译后修饰
Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2065 Human
- Entrez Gene: 13867 Mouse
- Entrez Gene: 29496 Rat
- Omim: 190151 Human
- SwissProt: P21860 Human
- SwissProt: Q61526 Mouse
- SwissProt: Q62799 Rat
- Unigene: 118681 Human
see all -
别名
- c erbB 3 antibody
- c erbB3 antibody
- Erb b2 receptor tyrosine kinase 3 antibody
see all
图片
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All lanes : Anti-ErbB3 / HER3 (phospho Y1328) antibody [EPR5809] (ab133459) at 1/1000 dilution
Lane 1 : T47-D cell lysate untreated
Lane 2 : T47-D cell lysate treated with Neuregulin
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 148 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab133459 被引用在 3 文献中.
- Hu X et al. Sustained ErbB Activation Causes Demyelination and Hypomyelination by Driving Necroptosis of Mature Oligodendrocytes and Apoptosis of Oligodendrocyte Precursor Cells. J Neurosci 41:9872-9890 (2021). PubMed: 34725188
- Sun Y et al. Angiotensin II inhibits apoptosis of mouse aortic smooth muscle cells through regulating the circNRG-1/miR-193b-5p/NRG-1 axis. Cell Death Dis 10:362 (2019). PubMed: 31043588
- Chen J et al. A role for ErbB signaling in the induction of reactive astrogliosis. Cell Discov 3:17044 (2017). PubMed: 29238610