Anti-ErbB3 / HER3 (phospho Y1328)抗体(ab131444)
Key features and details
- Rabbit polyclonal to ErbB3 / HER3 (phospho Y1328)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ErbB3 / HER3 (phospho Y1328)抗体
参阅全部 ErbB3 / HER3 一抗 -
描述
兔多克隆抗体to ErbB3 / HER3 (phospho Y1328) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human ErbB3/ HER3 (phospho Y1328) conjugated to keyhole limpet haemocyanin. Synthetic phosphopeptide conjugated to KLH, surrounding the phosphorylation site of Tyrosine 1328 (
P-D-Y(p)-W-H
) of Human ErbB3/ HER3. -
阳性对照
- A431 cell extract treated with EGF; MCF7 cells; Human breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab131444 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131444于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 185 kDa (predicted molecular weight: 148 kDa).
|
|
IHC-P |
1/50 - 1/100.
|
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 185 kDa (predicted molecular weight: 148 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Binds and is activated by neuregulins and NTAK. -
组织特异性
Epithelial tissues and brain. -
疾病相关
Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
发展阶段
Overexpressed in a subset of human mammary tumors. -
结构域
The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. -
翻译后修饰
Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2065 Human
- Omim: 190151 Human
- SwissProt: P21860 Human
- Unigene: 118681 Human
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别名
- c erbB 3 antibody
- c erbB3 antibody
- Erb b2 receptor tyrosine kinase 3 antibody
see all
图片
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All lanes : Anti-ErbB3 / HER3 (phospho Y1328) antibody (ab131444) at 1/500 dilution
Lane 1 : A431 cell extract, untreated
Lane 2 : A431 cell extract, treated with EGF
Predicted band size: 148 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1328) antibody (ab131444)
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling ErbB3 / HER3 (phospho Y1328) with ab131444 antibody at 1/50 dilution. The image on the right is treated with the synthesized peptide.
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Immunofluorescence of methanol-fixed MCF7 cells labelling EErbB3 / HER3 (phospho Y1328) with ab131444 antibody at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab131444 被引用在 2 文献中.
- Yoshizawa R et al. Biphasic spatiotemporal regulation of GRB2 dynamics by p52SHC for transient RAS activation. Biophys Physicobiol 18:1-12 (2021). PubMed: 33665062
- Yoshizawa R et al. p52Shc regulates the sustainability of ERK activation in a RAF-independent manner. Mol Biol Cell 32:1838-1848 (2021). PubMed: 34260260