重组Anti-ErbB2 / HER2 (phospho Y1139)抗体[EP1046Y] (ab53290)
![Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)](https://www.abcam.cn/ps/products/53/ab53290/Images/ab53290-255420-anti-erbb-2-phospho-y1139-antibody-ep1046y-western-blot.jpg "Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1046Y] to ErbB2 / HER2 (phospho Y1139)
- Suitable for: WB, IP, Dot blot
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ErbB2 / HER2 (phospho Y1139)抗体[EP1046Y]
参阅全部 ErbB2 / HER2 一抗 -
描述
兔单克隆抗体[EP1046Y] to ErbB2 / HER2 (phospho Y1139) -
宿主
Rabbit -
经测试应用
适用于: WB, IP, Dot blotmore details
不适用于: ICC/IF,IHC-Fr or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: SKBR3 and A431 cell lysates treated with EGF.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1046Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53290于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 185 kDa (predicted molecular weight: 138 kDa).
|
| IP |
1/30.
|
|
| Dot blot |
1/1000.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 185 kDa (predicted molecular weight: 138 kDa). |
|
IP
1/30. |
|
Dot blot
1/1000. |
应用说明
Is unsuitable for ICC/IF,IHC-Fr or IHC-P.
靶标
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功能
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. -
组织特异性
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. -
疾病相关
Hereditary diffuse gastric cancer
Glioma
Ovarian cancer
Lung cancer
Gastric cancer
Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). -
细胞定位
Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. - Information by UniProt
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数据库链接
- Entrez Gene: 2064 Human
- Omim: 164870 Human
- SwissProt: P04626 Human
- Unigene: 446352 Human
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别名
- Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
- C erb B2/neu protein antibody
- CD340 antibody
see all
图片
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Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)All lanes : Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290) at 1/500 dilution
Lane 1 : A431 whole cell lysate - untreated
Lane 2 : A431 whole cell lysate - treated with Epidermal Growth Factor (EGF)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 138 kDa
Observed band size: 185 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)](https://www.abcam.cn/ps/products/53/ab53290/Images/ab53290-272009-anti-erbb-2-phospho-y1139-antibody-ep1046y-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)ab53290 at 1/70 dilution immunoprecipitating ErbB 2 (phospho Y1139) in A431(human epidermoid carcinoma) whole cell lysate.
Lane 1 (input): A431treated with 100 ng/mL EGF for 10 minutes whole cell lysate 10μg
Lane 2 (+): ab53290 + A431 treated with 100 ng/mL EGF for 10 minutes whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab53290 in A431treated with 100 ng/mL EGF for 10 minutes whole cell lysate
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Blocking and Diluting buffer and concentration: 5% NFDM/TBST.
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![Dot Blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)](https://www.abcam.cn/ps/products/53/ab53290/Images/ab53290-255419-anti-erbb-2-phospho-y1139-antibody-ep1046y-dot-blot.jpg)
Dot Blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)Dot blot analysis of ErbB 2 (pY1139) peptide (Lane 1) and ErbB 2 non-phospho peptide (Lane 2) labelling ErbB 2 (phospho Y1139) with ab53290 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)](https://www.abcam.cn/ps/products/53/ab53290/Images/ab53290-66080-anti-erbb-2-phospho-y1139-antibody-ep1046y-western-blot.jpg)
Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)All lanes : Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290) at 1/1000 dilution
Lane 1 : SKBR3 cell lysate
Lane 2 : SKBR3 cell lysate - treated with EGF
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 138 kDa
Observed band size: 185 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)](https://www.abcam.cn/ps/products/53/ab53290/Images/ab53290-66079-anti-erbb-2-phospho-y1139-antibody-ep1046y-western-blot.jpg)
Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)This image is courtesy of an Abreview by Surekha Pimple.All lanes : Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290) at 1/2000 dilution
Lane 1 : HTB-2O Human breast ductal carcinoma lysate untreated
Lanes 2-9 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated Mouse anti-Rabbit monoclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 138 kDa
Exposure time: 5 seconds
Lanes 2-9 are treated with an increasing concentration of HER2 inhibitor.
Blocking performed with 5% Milk for 1 hour at room temperature.
Antibody diluted in CPPT and incubated for 16 hours at 4°C.
Performed under denaturing conditions. -
![Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)](https://www.abcam.cn/ps/products/53/ab53290/Images/ab53290-6-benefits-of-recombinant-antibodies.png)
Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab53290 被引用在 15 文献中.
- Padmanabhan R et al. Quantification of the growth suppression of HER2+ breast cancer colonies under the effect of trastuzumab and PD-1/PD-L1 inhibitor. Front Oncol 12:977664 (2022). PubMed: 36568154
- Drake JM et al. Regulation of a Novel Splice Variant of Early Growth Response 4 (EGR4-S) by HER+ Signalling and HSF1 in Breast Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 35326716
- Pereira PMR et al. Immuno-PET Detects Changes in Multi-RTK Tumor Cell Expression Levels in Response to Targeted Kinase Inhibition. J Nucl Med 62:366-371 (2021). PubMed: 32646879
- Yoshizawa R et al. Biphasic spatiotemporal regulation of GRB2 dynamics by p52SHC for transient RAS activation. Biophys Physicobiol 18:1-12 (2021). PubMed: 33665062
- Yoshizawa R et al. p52Shc regulates the sustainability of ERK activation in a RAF-independent manner. Mol Biol Cell 32:1838-1848 (2021). PubMed: 34260260