重组Anti-ErbB2 / HER2抗体[ICR12] (ab11710)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)](https://www.abcam.cn/ps/products/11/ab11710/Images/ab11710-1-anti-erbb-2-antibody-icr12-immunohistochemistry-human-breast-carcinoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [ICR12] to ErbB2 / HER2
- Suitable for: IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ErbB2 / HER2抗体[ICR12]
参阅全部 ErbB2 / HER2 一抗 -
描述
大鼠单克隆抗体[ICR12] to ErbB2 / HER2 -
宿主
Rat -
经测试应用
适用于: IHC-P, ICC/IF, Flow Cytmore details
不适用于: IP or WB -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human breast carcinoma tissue. ICC/IF: SK-BR-3 cells. Flow Cyt: SK-BR-3 cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Ion Exchange Chromatography -
克隆
单克隆 -
克隆编号
ICR12 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11710于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/100.
|
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| Flow Cyt |
1/1000.
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| 说明 |
|---|
|
IHC-P
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/100. |
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Flow Cyt
1/1000. |
靶标
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功能
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. -
组织特异性
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. -
疾病相关
Hereditary diffuse gastric cancer
Glioma
Ovarian cancer
Lung cancer
Gastric cancer
Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). -
细胞定位
Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. - Information by UniProt
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数据库链接
- Entrez Gene: 2064 Human
- Omim: 164870 Human
- SwissProt: P04626 Human
- Unigene: 446352 Human
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别名
- Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
- C erb B2/neu protein antibody
- CD340 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling ErbB2 / HER2 with ab11710 at 1/400 dilution followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Membranous staining on human breast carcinoma. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)](https://www.abcam.cn/ps/products/11/ab11710/Images/ab11710-3-anti-erbb-2-antibody-icr12-immunocytochemistry-skbr3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-BR-3 cells labelling ErbB 2 with ab11710 at 1/100 dilution, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong membranous staining in SK-BR-3 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 diliution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab11710 at 1/100 dilution followed by ab150080 at a 1/1000 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.
Negative control cells: MCF7 (PMID: 18288420).
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![Flow Cytometry - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)](https://www.abcam.cn/ps/products/11/ab11710/Images/ab11710-4-anti-erbb-2-antibody-icr12-flow-cytometry-mcf7.jpg)
Flow Cytometry - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left panel) / SK-BR-3 (human breast adenocarcinoma epithelial cell, Right panel) cells labelling ErbB 2 with ab11710 at 1/1000 dilution (0.1µg) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rat IgG (Alexa Fluor® 488, ab150157) at 1/2000 dilution was used as the secondary antibody.
Low expression control: MCF7 (PMID: 17938260).
Gated on viable cells.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)](https://www.abcam.cn/ps/products/11/ab11710/Images/ab11710-2-anti-erbb-2-antibody-icr12-immunohistochemistry-human-breast-carcinoma1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling ErbB 2 with ab11710 at 1/400 dilution followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). No staining on human breast carcinoma without expression of HER2 is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)](https://www.abcam.cn/ps/products/11/ab11710/Images/ab11710-5-benefits-of-recombinant-antibodies.png)
Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab11710 被引用在 3 文献中.
- Zheng G et al. Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab. Signal Transduct Target Ther 6:236 (2021). PubMed: 34158475
- Smellie WJ et al. Radioimmunotherapy of breast cancer xenografts with monoclonal antibody ICR12 against c-erbB2 p185: comparison of iodogen and N-succinimidyl 4-methyl-3-(tri-n-butylstannyl)benzoate radioiodination methods. Cancer Res 55:5842s-5846s (1995). PubMed: 7493357
- Eccles SA et al. Regression of established breast carcinoma xenografts with antibody-directed enzyme prodrug therapy against c-erbB2 p185. Cancer Res 54:5171-7 (1994). PubMed: 7923136