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Anti-ErbB2 / HER2抗体[CB11] (ab8054)

Submit a review Q&A (3)References (28)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CB11] (ab8054)

    Key features and details

    • Mouse monoclonal [CB11] to ErbB2 / HER2
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-ErbB2 / HER2抗体[CB11]
      参阅全部 ErbB2 / HER2 一抗

    • 描述

      小鼠单克隆抗体[CB11] to ErbB2 / HER2

    • 宿主

      Mouse

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Synthetic peptide corresponding to ErbB2/ HER2.

    • 阳性对照

      • Adenocarcinoma of the breast

    • 常规说明

      C-erbB-2 oncogene codes for a membrane protein, Neu protein. In several studies the amplification of C-erbB-2 oncogene in carcinomas has been reported. Overexpression of the Neu oncogene protein can be demonstrated by this antibody.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab8054于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    Use at an assay dependent concentration.
    说明
    IHC-P
    Use at an assay dependent concentration.

    靶标

    • 功能

      Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
      In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

    • 组织特异性

      Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

    • 疾病相关

      Hereditary diffuse gastric cancer
      Glioma
      Ovarian cancer
      Lung cancer
      Gastric cancer
      Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.

    • 序列相似性

      Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.

    • 翻译后修饰

      Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).

    • 细胞定位

      Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
        • C erb B2/neu protein antibody
        • CD340 antibody
        • CD340 antigen antibody
        • Cerb B2/neu protein antibody
        • CerbB2 antibody
        • Erb b2 receptor tyrosine kinase 2 antibody
        • ErbB-2 proto-oncogene antibody
        • ERBB2 antibody
        • ERBB2_HUMAN antibody
        • HER 2 antibody
        • HER 2/NEU antibody
        • HER2 antibody
        • Herstatin antibody
        • Human epidermal growth factor receptor 2 antibody
        • Metastatic lymph node gene 19 protein antibody
        • MLN 19 antibody
        • MLN19 antibody
        • NEU antibody
        • NEU proto oncogene antibody
        • Neuro/glioblastoma derived oncogene homolog antibody
        • Neuroblastoma/glioblastoma derived oncogene homolog antibody
        • NGL antibody
        • p185erbB2 antibody
        • Proto-oncogene c-ErbB-2 antibody
        • Proto-oncogene Neu antibody
        • Receptor tyrosine-protein kinase erbB-2 antibody
        • TKR1 antibody
        • Tyrosine kinase type cell surface receptor HER2 antibody
        • Tyrosine kinase-type cell surface receptor HER2 antibody
        • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody
        • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 antibody
        • V erb b2 avian erythroblastic leukemia viral oncoprotein 2 antibody
        • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
        • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
        • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CB11] (ab8054)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CB11] (ab8054)

        ab8054 labelling ErbB2 / HER2 in human breast; invasive ductal carcinoma.

      文献 (28)

      发表研究结果有使用 ab8054？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab8054 被引用在 28 文献中.

      • Dolapçi İB  et al. miR-216b-5p promotes late apoptosis/necroptosis in trastuzumab-resistant SK-BR-3 cells. Turk J Biol 47:199-207 (2023). PubMed: 37529418
      • Warren EAK  et al. Human epidermal growth factor receptor 2 expression in head and neck squamous cell carcinoma: Variation within and across primary tumor sites, and implications for antigen-specific immunotherapy. Head Neck 43:1983-1994 (2021). PubMed: 33660372
      • Orfanou IM  et al. Discovery and Pharmacological Evaluation of STEAP4 as a Novel Target for HER2 Overexpressing Breast Cancer. Front Oncol 11:608201 (2021). PubMed: 33842315
      • Bartkowiak K  et al. Cysteine-Rich Angiogenic Inducer 61: Pro-Survival Function and Role as a Biomarker for Disseminating Breast Cancer Cells. Cancers (Basel) 13:N/A (2021). PubMed: 33540545
      • Gameiro A  et al. HER2-Targeted Immunotherapy and Combined Protocols Showed Promising Antiproliferative Effects in Feline Mammary Carcinoma Cell-Based Models. Cancers (Basel) 13:N/A (2021). PubMed: 33919468
      View all Publications for this product

      客户评价及客户问答

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      1-3 of 3 Abreviews or Q&A

      Question

      Dear All,

      Please recommend for us positive control slide form Abcam for those Abs below:

      ab16901, ab8054, ab2428

      Read More
      Answer

      Thank you for your enquiry.

      I can confirm that ErbB2 is known to be expressed in breast issue and highly expressed in many breast cancers. Breast tumor tissue would therefore be a positive control to consider. You may want to do consider some further literature searching for more information:

      http://www.ncbi.nlm.nih.gov/pubmed/1977679

      We have some breast tumor tissue slides available:

      ab4697 Breast tumor (human): ductal carcinoma (invasive) tissue slides
      https://www.abcam.com/Breast-tumor-human-ductal-carcinoma-invasive-tissue-slides-ab4697.html (or use the following: https://www.abcam.com/Breast-tumor-human-ductal-carcinoma-invasive-tissue-slides-ab4697.html).

      The Unigene EST expression profile indicates it is expressed in many other tissues aswell:

      http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.446352

      We have many slides available, please see the following link for a list of the slides we sell:

      https://www.abcam.com/index.html?c=2300

      I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

      Read More

      Question

      Incorrect staining with these antibodies.

      Read More
      Answer

      Thanks for your phone calls and for letting us know about the trouble with these antibodies. Your credit note ID is ***. I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. Please let me know if there is anything else that we can do for you. I wish you the best of luck with your research!

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      Question

      I am looking for antibody against ErbB2 and ErbB2 antigen for my research on breast cancer. I'd like to buy an ErbB2 antibody (code:ab8054)from your company, so I need an antigen for the antibody, too. ErbB2 peptide (code:ab2429) is a suitable for ErbB2 antibody (code:ab8054)? or Do you have any suitable ErbB2 antigen in your company?

      Read More
      Answer

      Thank you for your enquiry. Ab2429 is the ErbB2 peptide used to generate Ab2428 - Rabbit polyclonal to ErbB2. It is a human C terminal synthetic peptide with the sequence of: KGTPTAENPEYLGLDVPV For ab8054, we don't see the antigen. If you have any additional questions, please contact us again.

      Read More

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      For licensing inquiries, please contact partnerships@abcam.com