Anti-ERAB抗体[5F3] (ab10260)

Thank you for your email. Unfortunately, we do not know the exact epitope that this product recognizes. We have not done an epitope mapping project for this yet. I apologize for the inconvenience.

The application was added to the datasheet based on publication in the following reference:

Stevanin G et al. Mutations in SPG11, encoding spatacsin, are a major cause of spastic paraplegia with thin corpus callosum. Nat Genet 39:366-372 (2007) (PubMed: 17322883).

The antibody was also used in the following reference, for IF (Figure 2) as well as western blotting.

Onoguchi K et al. Virus-infection or 5'ppp-RNA activates antiviral signal through redistribution of IPS-1 mediated by MFN1. PLoS Pathog 6:e1001012 (2010). (PubMed: 20661427).

The protocol in the paper is as follows:

For immunofluorescence analysis, cells were fixed with 4% paraformaldehyde for 10 min, permeabilized with an acetone: methanol (11) solution, and blocked with 5mg/ml of BSA in PBST (PBS, 0.04% Tween20) for 1 hour. The cells were next incubated with relevant primary antibodies overnight at 4°C, and then incubated with relevant Alexa Fluor 405, Alexa Fluor 488, or Alexa Fluor 594-conjugated secondary antibodies.

We are not aware of any data from staining of monkey samples.

Thank you for your enquiry. I am sorry that unfortunately, we are unable to supply this anti-ERAB antibody (ab10260) without sodium azide. We do, however, have another anti-ERAB antibody available that contains no preservatives. This is a Mouse monoclonal [10D12] to ERAB, ab17298. Please be aware that non of these antibodies have been tested for your in vivo application. I hope this helps. Please don't hesitate to get back to me if you have any further questions.

Thank you for your email. ERAB is identical to SCHAD. The following reference that I found explains this best: Characterization and localization of human type10 17-hydroxysteroid dehydrogenase; Eur. J. Biochem. 268, 4899-4907 (2001). In it the authors state "Human short chain L-3-hydroxyacyl-CoA dehydrogenase (SCHAD) is encoded by the SCHAD gene [10]. Human SCHAD is a member of the SDR family [17] but not the L-3-hydroxyacyl-CoA dehydrogenase (HAD) family [34], because it does not have a signature sequence that defines this family [27,35]. The use of term SCHAD to refer to members of the HAD family has been discouraged because it is inaccurate [36]. It exhibits oxidative 17/3-hydroxysteroid dehydrogenase activities toward sex steroids [11], which leads us to designate this 108-kDa protein as 17-HSD type 10. Because this protein exhibits a high affinity for A peptide and was reported to be associated with the ER, it had been referred to as ER-associated A binding protein (ERAB) [18], and was thought to be a putative A receptor. However, we found that SCHAD is present in mitochondria [11,19]. If this protein is specifically targeted to mitochondria, it seems unlikely that it could readily bind A generated in the ER as had been postulated previously." If you have any additional questions, please contact us again.

Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused. If you have any more questions, please do contact us again.