Anti-EPO抗体(ab273070)
Key features and details
- Rabbit polyclonal to EPO
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-EPO抗体
参阅全部 EPO 一抗 -
描述
兔多克隆抗体to EPO -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Rat -
免疫原
Recombinant full length protein corresponding to Human EPO.
Database link: P01588 -
阳性对照
- IHC-P: Rat brain and liver tissue. WB: Recombinant human EPO protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273070于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/5000 - 1/20000. Predicted molecular weight: 21 kDa.
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| 说明 |
|---|
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/5000 - 1/20000. Predicted molecular weight: 21 kDa. |
靶标
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相关性
Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. -
细胞定位
Secreted -
数据库链接
- Entrez Gene: 24335 Rat
- SwissProt: P29676 Rat
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别名
- EP antibody
- EPO alpha antibody
- EPO antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody (ab273070)
Paraffin-embedded rat liver tissue stained for EPO using ab273070 at 1/400 in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
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Western blot - Anti-EPO antibody (ab273070)All lanes : Anti-EPO antibody (ab273070) at 1/20000 dilution
Lane 1 : Recombinant human EPO protein, 20 ng
Lane 2 : Recombinant human EPO protein, 100 ng
Predicted band size: 21 kDa12% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody (ab273070)Paraffin-embedded rat brain tissue stained for EPO using ab273070 at 1/400 in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab273070 被引用在 2 文献中.
- Tang Z et al. Influences and mechanism of erythropoietin on the cognitive function of vascular dementia rats. Aging (Albany NY) 15:12264-12274 (2023). PubMed: 37934569
- Fu X et al. Qi-Long-Tian formula extract alleviates symptoms of acute high-altitude diseases via suppressing the inflammation responses in rat. Respir Res 22:52 (2021). PubMed: 33579290