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AB214192

Anti-epithelial Sodium Channel alpha抗体

Anti-epithelial Sodium Channel alpha antibody

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(4 Publications)

Rabbit Polyclonal epithelial Sodium Channel alpha antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SCNN1A aa 200-250 conjugated to Keyhole Limpet Haemocyanin.

查看别名

SCNN1, SCNN1A, Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Epithelial Na(+) channel subunit alpha, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA, Alpha-ENaC, ENaCA

2 Images
Western blot - Anti-epithelial Sodium Channel alpha antibody (AB214192)
  • WB

Supplier Data

Western blot - Anti-epithelial Sodium Channel alpha antibody (AB214192)

Rat kidney lysate (40 ug)

Primary : ab214192 at 1/1000 dilution

Secondary : IRDye800CW Goat anti-Rabbit IgG at 1/20000 dilution

Predicted band size : 76 kD

Observed band size : 77 kD

All lanes:

Western blot - Anti-epithelial Sodium Channel alpha antibody (ab214192)

Predicted band size: 75 kDa

false

Western blot - Anti-epithelial Sodium Channel alpha antibody (AB214192)
  • WB

Supplier Data

Western blot - Anti-epithelial Sodium Channel alpha antibody (AB214192)

Mouse kidney lysate (40 ug)

Primary : ab214192 at 1/1000 dilution

Secondary : IRDye800CW Goat anti-Rabbit IgG at 1/20000 dilution

Predicted band size : 76 kD

Observed band size : 77 kD

All lanes:

Western blot - Anti-epithelial Sodium Channel alpha antibody (ab214192)

Predicted band size: 75 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat

应用

WB

applications

免疫原

Synthetic Peptide within Human SCNN1A aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P37088

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The epithelial sodium channel alpha also known as the alpha subunit of the epithelial sodium channel (ENaC) is an important component of the ENaC channels. This protein forms part of the larger ENaC complex and plays an important role in sodium ion transport across epithelial cells. Typically the ENaC channel has a molecular mass of approximately 70 to 110 kDa. The channel is expressed in epithelial tissues such as the kidney lung and colon where it acts to regulate sodium excretion and reabsorption.
Biological function summary

The epithelial sodium channel alpha facilitates sodium ion entry into cells which impacts fluid balance blood pressure and extracellular fluid volume. The alpha subunit functions as part of a heterotrimeric complex consisting of alpha beta and gamma subunits forming the complete active channel. The coordinated action of these subunits ensures regulated ion transport which is essential for maintaining homeostasis in epithelial tissues.

Pathways

ENaC channels participate in the renin-angiotensin-aldosterone system and are involved in sodium reabsorption processes. This channel works closely with proteins like aldosterone to modulate its activity in response to changes in body fluid status. ENaC's ability to regulate sodium flux links it to key physiological functions in fluid and electrolyte balance pathways.

Alterations in ENaC function are linked to conditions such as Liddle's syndrome and cystic fibrosis. Liddle's syndrome involves mutations that lead to excessive sodium reabsorption resulting in hypertension. Furthermore ENaC function is disrupted in cystic fibrosis due to altered protein interactions contributing to imbalanced mucus composition. Proteins like cystic fibrosis transmembrane conductance regulator (CFTR) interact with ENaC in cystic fibrosis illustrating their interconnected role in disease pathology.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and eccrine sweat glands. Also plays a role in taste perception.
See full target information SCNN1A

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Hypertension research : official journal of the Japanese Society of Hypertension 48:2218-2233 PubMed40490489

2025

Dark-phase melatonin administration does not reduce blood pressure but induces changes in parameters related to the control of the cardiovascular system in spontaneously hypertensive rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Mauer Sutovska,Lubos Molcan,Peter Stefanik,Michal Zeman

Annals of translational medicine 11:4 PubMed36760249

2023

Involvement of miR-495 overexpression in the pathogenesis of bronchopulmonary dysplasia in preterm infants via the targeting of pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Fan Sun,Li Ma,Jian-Hui Li,Yuan Yang,Xiao-Hui Gong,Cheng Cai

Bioengineered 12:9390-9400 PubMed34823420

2021

Knocking down Sterol regulatory element binding protein 2 (SREBF2) inhibits the Serine Protease 8 (PRSS8) /sodium channel epithelial 1alpha subunit (SCNN1A) axis to reduce the cell proliferation, migration and epithelial-mesenchymal transformation of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Cai,Yumei Zhang,Xing Peng

Oncotarget 11:3590-3600 PubMed33062195

2020

NRXN1 as a novel potential target of antibody-drug conjugates for small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Takuma Yotsumoto,Keita Maemura,Kousuke Watanabe,Yosuke Amano,Yoko Matsumoto,Koichi Zokumasu,Takahiro Ando,Masanori Kawakami,Hidenori Kage,Jun Nakajima,Yutaka Yatomi,Takahide Nagase,Daiya Takai
View all publications

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