Anti-Ephrin B2 (phospho Y316)抗体(ab119323)
Key features and details
- Rabbit polyclonal to Ephrin B2 (phospho Y316)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Ephrin B2 (phospho Y316)抗体 -
描述
兔多克隆抗体to Ephrin B2 (phospho Y316) -
宿主
Rabbit -
特异性
ab119323 recognizes endogenous Ephrin B2 only when phosphorylated at Tyrosine 316. May also react with Ephrin B1 and B3 as the immunogen sequence is also present in these proteins if phophorylated. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to Human Ephrin B2 aa 300-400 (phospho Y316) conjugated to keyhole limpet haemocyanin.
Database link: P52799 -
阳性对照
- HT29 cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119323于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 37 kDa.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 37 kDa. |
靶标
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功能
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
(Microbial infection) Acts as a receptor for Hendra virus and Nipah virus. -
组织特异性
Lung and kidney. -
序列相似性
Belongs to the ephrin family.
Contains 1 ephrin RBD (ephrin receptor-binding) domain. -
翻译后修饰
Inducible phosphorylation of tyrosine residues in the cytoplasmic domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1948 Human
- Entrez Gene: 306636 Rat
- Omim: 600527 Human
- SwissProt: P52799 Human
- Unigene: 149239 Human
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别名
- EFN B2 antibody
- EFNB 2 antibody
- Efnb2 antibody
see all
图片
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Western blot - Anti-Ephrin B2 (phospho Y316) antibody (ab119323)All lanes : Anti-Ephrin B2 (phospho Y316) antibody (ab119323) at 1/500 dilution
Lane 1 : HT29 cell lysate
Lane 2 : HT29 cells, treated with UV and lysed
Predicted band size: 37 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab119323 被引用在 3 文献中.
- Wang T et al. Activation of EphrinB2 Signaling Promotes Adaptive Venous Remodeling in Murine Arteriovenous Fistulae. J Surg Res 262:224-239 (2021). PubMed: 33039109
- Zhu S et al. Bidirectional ephrinB2-EphB4 signaling regulates the osteogenic differentiation of canine periodontal ligament stem cells. Int J Mol Med 45:897-909 (2020). PubMed: 31985015
- Zhang G et al. EphB4 forward signalling regulates lymphatic valve development. Nat Commun 6:6625 (2015). WB . PubMed: 25865237