Anti-Ephrin A2抗体[OTI3E3] (ab123877)
![Immunocytochemistry/ Immunofluorescence - Anti-Ephrin A2 antibody [OTI3E3] (ab123877)](https://www.abcam.cn/ps/products/123/ab123877/Images/ab123877-137053-anti-ephrin-a2-antibody-oti3e3-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Ephrin A2 antibody [OTI3E3] (ab123877)")
Key features and details
- Mouse monoclonal [OTI3E3] to Ephrin A2
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG2b
概述
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产品名称
Anti-Ephrin A2抗体[OTI3E3] -
描述
小鼠单克隆抗体[OTI3E3] to Ephrin A2 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human Ephrin A2 aa 1-213. Produced in HEK293T cells (NP_001396).
Sequence:MAPAQRPLLPLLLLLLPLPPPPFARAEDAARANSDRYAVYWNRSNPRFHA GAGDDGGGYTVEVSINDYLDIYCPHYGAPLPPAERMEHYVLYMVNGEGHA SCDHRQRGFKRWECNRPAAPGGPLKFSEKFQLFTPFSLGFEFRPGHEYYY ISATPPNAVDRPCLRLKVYVRPTNETLYEAPEPIFTSNNSCSSPGGCRLF LSTIPVLWTLLGS
Database link: O43921 -
阳性对照
- WB: HEK293T cells transfected pCMV6-ENTRY Ephrin A2 cDNA. IHC-P: Human endometrium adenocarcinoma tissue. ICC/IF:COS7 cells transiently transfected by pCMV6-ENTRY Ephrin A2. Flow Cyt (Intra): Jurkat cells.
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常规说明
The clone number has been updated from 3E3 to OTI3E3, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography -
克隆
单克隆 -
克隆编号
OTI3E3 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab123877于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/2000. Predicted molecular weight: 24 kDa.
|
|
| IHC-P |
1/150.
|
|
| ICC/IF |
1/100.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/100. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
WB
1/2000. Predicted molecular weight: 24 kDa. |
|
IHC-P
1/150. |
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ICC/IF
1/100. |
靶标
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序列相似性
Belongs to the ephrin family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1943 Human
- Omim: 602756 Human
- SwissProt: O43921 Human
- Unigene: 532655 Human
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别名
- EFNA 2 antibody
- efna2 antibody
- EFNA2_HUMAN antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Ephrin A2 antibody [OTI3E3] (ab123877)
COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY Ephrin A2, stained for Epherin A2 (Green) using ab123877 at 1/100 dilution in ICC/IF.
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![Western blot - Anti-Ephrin A2 antibody [OTI3E3] (ab123877)](https://www.abcam.cn/ps/products/123/ab123877/Images/ab123877-137055-anti-ephrin-a2-antibody-oti3e3-western-blot.jpg)
Western blot - Anti-Ephrin A2 antibody [OTI3E3] (ab123877)All lanes : Anti-Ephrin A2 antibody [OTI3E3] (ab123877) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY Ephrin A2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 24 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin A2 antibody [OTI3E3] (ab123877)](https://www.abcam.cn/ps/products/123/ab123877/Images/ab123877-137054-anti-ephrin-a2-antibody-oti3e3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin A2 antibody [OTI3E3] (ab123877)Paraffin-embedded human endometrium tissue stained for Ephrin A2 using ab123877 at 1/100 dilution in immunohistochemical analysis.
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![Flow Cytometry (Intracellular) - Anti-Ephrin A2 antibody [OTI3E3] (ab123877)](https://www.abcam.cn/ps/products/123/ab123877/Images/ab123877-137052-anti-ephrin-a2-antibody-oti3e3-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Ephrin A2 antibody [OTI3E3] (ab123877)ab123877 at 1/100 dilution staining Ephrin-A2 in Jurkat cells by Flow Cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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