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AB123877

Anti-Ephrin A2抗体[OTI3E3]

Anti-Ephrin A2 antibody [OTI3E3]

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(4 Publications)

Mouse Monoclonal Ephrin A2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, African green monkey samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EFNA2.

查看别名

EPLG6, LERK6, EFNA2, Ephrin-A2, EPH-related receptor tyrosine kinase ligand 6, HEK7 ligand, LERK-6, HEK7-L

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin A2 antibody [OTI3E3] (AB123877)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin A2 antibody [OTI3E3] (AB123877)

Paraffin-embedded human endometrium tissue stained for Ephrin A2 using ab123877 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-Ephrin A2 antibody [OTI3E3] (AB123877)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Ephrin A2 antibody [OTI3E3] (AB123877)

ab123877 at 1/100 dilution staining Ephrin-A2 in Jurkat cells by Flow Cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-Ephrin A2 antibody [OTI3E3] (AB123877)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ephrin A2 antibody [OTI3E3] (AB123877)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY Ephrin A2, stained for Epherin A2 (Green) using ab123877 at 1/100 dilution in ICC/IF.

Western blot - Anti-Ephrin A2 antibody [OTI3E3] (AB123877)
  • WB

Unknown

Western blot - Anti-Ephrin A2 antibody [OTI3E3] (AB123877)

All lanes:

Western blot - Anti-Ephrin A2 antibody [OTI3E3] (ab123877) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cells transfected with pCMV6-ENTRY Ephrin A2 cDNA at 5 µg

Predicted band size: 24 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

OTI3E3

亚型

IgG2b

不含载体蛋白

No

反应种属

Human, African green monkey

应用

ICC/IF, IHC-P, Flow Cyt (Intra), WB

applications

免疫原

Recombinant Full Length Protein corresponding to Human EFNA2.

O43921

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>" }, "African green monkey": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

产品详情

The clone number has been updated from 3E3 to OTI3E3, both clone numbers name the same clone.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
纯化说明
Purified from cell culture supernatant by affinity chromatography
存储溶液
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Ephrin A2 also known as EFNA2 is a member of the ephrin family which plays an important role in cellular processes. This protein is a glycosylphosphatidylinositol (GPI)-anchored ligand for Eph receptors which are a type of receptor tyrosine kinases. Ephrin A2 has a mass of approximately 25 kDa. It is mostly expressed in the nervous system as well as in the endothelial cells of blood vessels. Ephrin A2 is important for mediating cell signaling that affects morphology and cellular movement.
Biological function summary

Ephrin A2 is involved in cell positioning and boundary formation. It is essential during embryonic development for tissue patterning. The ephrin A2 binds to Eph receptors often forming a complex that triggers bidirectional signaling. This complex affects neuronal growth cones guiding axon pathfinding and is significant in neurogenesis. Its signaling impacts cellular adhesion and repulsion which regulates how cells interact with their surroundings and other cells.

Pathways

Ephrin A2 is integrated into the Eph/ephrin signaling pathway which is important for various cellular responses. This signaling impacts the Rho family of GTPases which play a role in mediating cytoskeletal dynamics. Ephrin A2 also interacts with the MAPK/ERK pathway contributing to the regulation of cell proliferation and differentiation. Within these pathways it associates with proteins like EphA4 and ephrin A5 influencing pathway outcomes.

Ephrin A2 has associations with cancer particularly in tumor growth and angiogenesis. Altered Ephrin A2 signaling can contribute to the progression of cancers by affecting vascularization and cell migration. Additionally its dysregulation is linked to neurodegenerative conditions such as Alzheimer's disease. Through these diseases Ephrin A2 interacts with various proteins including amyloid precursor protein and tau which are involved in disease pathologies.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. With the EPHA2 receptor may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis (By similarity).
See full target information EFNA2

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:191 PubMed40413506

2025

EphrinA2 promotes glioma cell migration and invasion through EphA2 and FAK.

Applications

Unspecified application

Species

Unspecified reactive species

Nozomi Hirai,Sho Tamai,Toshiya Ichinose,Hemragul Sabit,Norihiko Saito,Satoshi Iwabuchi,Mitsutoshi Nakada

Diagnostics (Basel, Switzerland) 12: PubMed35626181

2022

EPHA2, EPHA4, and EPHA6 Expression in Uveal Melanomas: Searching for the Culprits of Neoplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandros Pergaris,Eugene Danas,Pawel Gajdzis,Georgia Levidou,Malgorzata Gajdzis,Nathalie Cassoux,Sophie Gardrat,Piotr Donizy,Penelope Korkolopoulou,Nikolaos Kavantzas,Jerzy Klijanienko,Stamatios Theocharis

Diagnostics (Basel, Switzerland) 11: PubMed34943502

2021

Ephrin Receptors (Ephs) Expression in Thymic Epithelial Tumors: Prognostic Implications and Future Therapeutic Approaches.

Applications

Unspecified application

Species

Unspecified reactive species

Christos Masaoutis,Natalia Georgantzoglou,Panagiotis Sarantis,Irene Theochari,Nikolaos Tsoukalas,Mattheos Bobos,Paraskevi Alexandrou,Alexandros Pergaris,Dimitra Rontogianni,Stamatios Theocharis

Pathology international 64:415-22 PubMed25243889

2014

Class III/IV POU transcription factors expressed in small cell lung cancer cells are involved in proneural/neuroendocrine differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Ishii,Hanako Sato,Takuya Yazawa,Yukiko Shishido-Hara,Chie Hiramatsu,Yukio Nakatani,Hiroshi Kamma
View all publications

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