重组Anti-Ephrin A1抗体[EPR1785(2)] (ab124911)
![Western blot - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)](https://www.abcam.cn/ps/products/124/ab124911/Images/ab124911-138902-anti-ephrin-a1-antibody-epr17852-western-blot.jpg "Western blot - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1785(2)] to Ephrin A1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Ephrin A1抗体[EPR1785(2)]
参阅全部 Ephrin A1 一抗 -
描述
兔单克隆抗体[EPR1785(2)] to Ephrin A1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide within Human Ephrin A1 aa 1-100 (N terminal). The exact sequence is proprietary.
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阳性对照
- HUVEC (treated with TNF alpha), HUVEC (untreated) and MCF7 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.59 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1785(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124911于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
靶标
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功能
Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cones. -
组织特异性
Brain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at protein level). -
序列相似性
Belongs to the ephrin family. -
翻译后修饰
Undergo proteolysis by a metalloprotease to give rise to a soluble monomeric form. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1942 Human
- Omim: 191164 Human
- SwissProt: P20827 Human
- Unigene: 516664 Human
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别名
- B61 antibody
- ECKLG antibody
- EFL 1 antibody
see all
图片
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Western blot - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)All lanes : Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911) at 1/1000 dilution
Lane 1 : HUVEC cell lysate, treated with TNF alpha
Lane 2 : HUVEC cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa -
![OI-RD Scanning - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)](https://www.abcam.cn/ps/products/124/ab124911/Images/ab124911-138901-anti-ephrin-a1-antibody-epr17852.jpg)
OI-RD Scanning - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911) -
![Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)](https://www.abcam.cn/ps/products/124/ab124911/Images/ab124911-3-benefits-of-recombinant-antibodies.png)
Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab124911 被引用在 4 文献中.
- Yi C et al. A three-dimensional spheroid-specific role for Wnt-β-catenin and Eph-ephrin signaling in nasopharyngeal carcinoma cells. J Cell Sci 134:N/A (2021). PubMed: 34338780
- Zhang Y et al. Effects of EFNA1 on cell phenotype and prognosis of esophageal carcinoma. World J Surg Oncol 19:242 (2021). PubMed: 34399788
- Shi ZZ et al. The miR-1224-5p/TNS4/EGFR axis inhibits tumour progression in oesophageal squamous cell carcinoma. Cell Death Dis 11:597 (2020). PubMed: 32732965
- Wang Y et al. HIC1 and miR-23~27~24 clusters form a double-negative feedback loop in breast cancer. Cell Death Differ 24:421-432 (2017). PubMed: 28009350