重组Anti-Eph receptor B3抗体[EPR8280] (ab133742)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8280] to Eph receptor B3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-Eph receptor B3抗体[EPR8280]
参阅全部 Eph receptor B3 一抗 -
描述
兔单克隆抗体[EPR8280] to Eph receptor B3 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Eph receptor B3 aa 100-200 (extracellular). The exact sequence is proprietary.
-
阳性对照
- WB: Jurkat, HepG2, HeLa, MOLT4, NIH3T3, Rat-1 and C6 cell lysates. Rat spinal cord tissue lysate; ICC/IF: Jurkat and HeLa cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解离常数(KD)
KD = 5.70 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8280 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133742于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
For unpurified use at 1/1000 - 1/10000. |
|
ICC/IF |
1/50.
For unpurified use at 1/100 - 1/250. |
说明 |
---|
WB
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). For unpurified use at 1/1000 - 1/10000. |
ICC/IF
1/50. For unpurified use at 1/100 - 1/250. |
靶标
-
功能
Receptor for members of the ephrin-B family. Binds to ephrin-B1 and -B2. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 2049 Human
- Entrez Gene: 13845 Mouse
- Entrez Gene: 287989 Rat
- Omim: 601839 Human
- SwissProt: P54753 Human
- SwissProt: P54754 Mouse
- Unigene: 2913 Human
- Unigene: 6972 Mouse
-
别名
- Cek10 antibody
- EK2 antibody
- Embryonic kinase 2 antibody
see all
图片
-
All lanes : Anti-Eph receptor B3 antibody [EPR8280] (ab133742) at 1/1000 dilution (Purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 7 : Rat spinal cord lysates
Lane 8 : Rat-1 (Rat embryonic fibroblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDaBlocking/Diluting buffer: 5% NFDM/TBST
-
Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Eph receptor B3 with Purified ab133742 at 1/50 dilution (5.14 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
All lanes : Anti-Eph receptor B3 antibody [EPR8280] (ab133742) at 1/1000 dilution (unpurified)
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : MOLT4 cell lysate
Lane 5 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 110 kDa -
Immunocytochemical analysis of HeLa cells labelling Eph receptor B3 with unpurified ab133742 at 1/100.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab133742 被引用在 5 文献中.
- Chakraborty C et al. Rewiring of the promoter-enhancer interactome and regulatory landscape in glioblastoma orchestrates gene expression underlying neurogliomal synaptic communication. Nat Commun 14:6446 (2023). PubMed: 37833281
- Kung A et al. A Chemical-Genetic Approach to Generate Selective Covalent Inhibitors of Protein Kinases. ACS Chem Biol 12:1499-1503 (2017). PubMed: 28459525
- Krusche B et al. EphrinB2 drives perivascular invasion and proliferation of glioblastoma stem-like cells. Elife 5:N/A (2016). PubMed: 27350048
- Murphy M et al. TCF7L1 Modulates Colorectal Cancer Growth by Inhibiting Expression of the Tumor-Suppressor Gene EPHB3. Sci Rep 6:28299 (2016). PubMed: 27333864
- Lin B et al. Interplay between chemotaxis and contact inhibition of locomotion determines exploratory cell migration. Nat Commun 6:6619 (2015). PubMed: 25851023