AB281926

重组Anti-Eph receptor A3抗体[EPR24479-136]

Anti-Eph receptor A3 antibody [EPR24479-136]

20ul selling size
RabMAb
Recombinant
了解详情

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Rabbit Recombinant Monoclonal Eph receptor A3 antibody. Suitable for WB and reacts with Mouse, Human samples.

查看别名

ETK, ETK1, HEK, TYRO4, EPHA3, Ephrin type-A receptor 3, EPH-like kinase 4, Tyrosine-protein kinase TYRO4, Tyrosine-protein kinase receptor ETK1, EK4, hEK4, Human embryo kinase, Eph-like tyrosine kinase 1

3 Images

Lab

Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (AB281926)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTEPHA3 is a glycoprotein and can be de-glycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID : 28415715)

Exposure time : Lane1-2 : 37 secondsLane3-4 : 15 seconds

All lanes:

Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926) at 1/1000 dilution

Lane 1:

22Rv1 (human prostate carcinoma epithelial cell), whole cell lysate at 15 µg

Lane 2:

22Rv1 whole cell lysate treated with PNGase F at 15 µg

Lane 3:

B16-F10 (Mouse skin melanoma), whole cell lysate at 15 µg

Lane 4:

B16-F10 whole cell lysate treated with PNGase F at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 110 kDa

Observed band size: 140-150 kDa

false

Lab

Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (AB281926)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTNegative control : HL-60 (PMID : Â 18814179).

Exposure time : 59 seconds

All lanes:

Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926) at 1/1000 dilution

Lane 1:

22Rv1 (human prostate carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HL-60 (human Acute Promyelocytic Leukemia promyeloblast), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 110 kDa

Observed band size: 150 kDa

false

Lab

Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (AB281926)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure times : Lanes 1-4 : 5.5 secs; Lane 5 : 26 secs.

All lanes:

Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926) at 1/1000 dilution

Lane 1:

His-tagged human EPHA3 recombinant protein at 0.01 µg

Lane 2:

His-tagged human EPHA2 recombinant protein at 0.01 µg

Lane 3:

His-tagged human EPHA4 recombinant protein at 0.01 µg

Lane 4:

His-tagged human EPHA7 recombinant protein at 0.01 µg

Lane 5:

His-tagged human EPHA5 recombinant protein at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 110 kDa

Observed band size: 80 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR24479-136

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Eph receptor A3 also known as EphA3 is a member of the ephrin receptor subfamily of protein-tyrosine kinases. This receptor with a mass of approximately 120 kDa plays a role in cell-cell communication mainly by interacting with ephrin-A ligands. EphA3 is expressed in various tissues including the nervous system developing tissues and some adult tissues like the lungs and prostate. Its localization varies with the developmental stage and tissue type reflecting its role in numerous processes.

Biological function summary

EphA3 influences cellular organization movement and positioning through its involvement in contact-dependent signaling. It acts as part of a larger receptor-ligand complex which mediates bidirectional signaling between cells. This interaction generally leads to changes in the cytoskeleton impacting cell shape and locomotion. EphA3’s signaling can regulate developmental processes such as axon guidance angiogenesis and tissue boundary formation.

Pathways

EphA3 modulates various important signaling pathways such as the PI3K/AKT pathway and the Rho family GTPase pathway. It interacts with proteins like Ephrin-A5 and other Eph receptors coordinating complex cellular signaling networks. These interactions contribute to the regulation of cell proliferation survival and migration influencing tissue patterning and organ development.

EphA3 has associations with cancer and neurological disorders. It has ties to various cancers like leukemia where it is implicated in proliferation and migration of malignant cells. In the context of neurological conditions EphA3 plays a role in neurodevelopmental disorders. In these diseases connections with other ephrin receptors and proteins involved in cell signaling become critical as they influence pathogenic pathways.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling (PubMed : 11870224, PubMed : 12794130). Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5 (By similarity). Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration (PubMed : 11870224). Also activated by EFNA1, inhibiting epithelial-to-mesenchymal transition of cardiac cells and playing a role in heart development (PubMed : 12794130). Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development (By similarity).

See full target information EPHA3

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Abcam Product Promise

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重组Anti-Eph receptor A3抗体[EPR24479-136]