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重组RabMAb

重组Anti-Eph receptor A2抗体[EPR17660-120] - BSA and Azide free (ab225579)

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Flow Cytometry - Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (ab225579)
  • Flow Cytometry - Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (ab225579)
  • Immunoprecipitation - Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (ab225579)
  • Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (ab225579)
  • Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (ab225579)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17660-120] to Eph receptor A2 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, IP, WB
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Unconjugated

概述

  • 产品名称

    Anti-Eph receptor A2抗体[EPR17660-120] - BSA and Azide free
    参阅全部 Eph receptor A2 一抗

  • 描述

    兔单克隆抗体[EPR17660-120] to Eph receptor A2 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt, ICC/IF, IP, WBmore details

  • 种属反应性

    与反应: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • ICC/IF: NIH/3T3 cells. Flow cyt: NIH3T3 cells

  • 常规说明

    ab225579 is the carrier-free version of ab185156.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab225579于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 109 kDa).
说明
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 109 kDa).

靶标

  • 功能

    Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5. Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Induces apoptosis in a p53/TP53-independent, caspase-8-dependent manner.

  • 组织特异性

    Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary.

  • 疾病相关

    Genetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2 (ARCC2) [MIM:613020]. A developmental punctate opacity common in the cortex and present in most lenses. The cataract is white or cerulean, increases in number with age, but rarely affects vision.
    Defects in EPHA2 are the cause of cataract posterior polar type 1 (CTPP1) [MIM:116600]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity.

  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.

  • 翻译后修饰

    Activated by EFNA1 via tyrosine phosphorylation. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit. These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and endothelial cell migration. They also play a critical role in transducing EPHA2 signaling in vascular endothelial cells during tumor angiogenesis.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ARCC2 antibody
      • AW545284 antibody
      • CTPA antibody
      • CTPP1 antibody
      • CTRCT6 antibody
      • EC 2.7.10.1 antibody
      • Eck antibody
      • Eph receptor A2 antibody
      • EPHA2 antibody
      • EPHA2_HUMAN antibody
      • Ephrin receptor antibody
      • Ephrin receptor EphA2 antibody
      • Ephrin type A receptor 2 antibody
      • Ephrin type-A receptor 2 antibody
      • Epithelial cell kinase antibody
      • Epithelial cell receptor protein tyrosine kinase antibody
      • Myk 2 antibody
      • Myk2 antibody
      • Sek 2 antibody
      • Sek2 antibody
      • Soluble EPHA2 variant 1 antibody
      • Tyrosine protein kinase receptor ECK antibody
      • Tyrosine-protein kinase receptor ECK antibody
      • Tyrosine-protein kinase receptor MPK-5 antibody
      • Tyrosine-protein kinase receptor SEK-2 antibody
      see all

    图片

    • Flow Cytometry - Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (ab225579)
      Flow Cytometry - Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (ab225579)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185156).

      Flow cytometry overlay histogram showing left NIH3T3 positive cells and right negative B16-F10 stained with ab185156 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interactionfollowed by the antibody (ab185156) (1x 106 in 100μl at 5.0 μg/ml (1/424)) for 30min on ice.

      The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice

      Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

    • Flow Cytometry - Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (ab225579)
      Flow Cytometry - Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (ab225579)

      Flow cytometric analysis of NIH/3T3 (mouse embryonic fibroblast cell line) cell line labeling Eph receptor A2 with ab185156 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

      Total viable cells were gated for the FC image.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185156).

    • Immunoprecipitation - Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (ab225579)
      Immunoprecipitation - Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (ab225579)

      Eph receptor A2 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryonic fibroblast cell line) lysate with ab185156 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab185156 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: NIH/3T3 whole cell lysate 10 µg (Input). 

      Lane 2: ab185156 IP in NIH/3T3 whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab185156 in NIH/3T3 whole cell lysate.

      Exposure time: 10 seconds.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185156).

    • Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (ab225579)
      Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (ab225579)

      Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (mouse embryonic fibroblast cell line) cells labeling Eph receptor A2 with ab185156  at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on NIH/3T3 cells.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185156).

    • Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (ab225579)
      Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (ab225579)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

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