重组Anti-EPB41L2抗体[EPR8873(2)] (ab175928)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L2 antibody [EPR8873(2)] (ab175928)](https://www.abcam.cn/ps/products/175/ab175928/Images/ab175928-202938-anti-epb4il2-antibody-epr88732-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L2 antibody [EPR8873(2)] (ab175928)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8873(2)] to EPB41L2
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-EPB41L2抗体[EPR8873(2)]
参阅全部 EPB41L2 一抗 -
描述
兔单克隆抗体[EPR8873(2)] to EPB41L2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, IPmore details
不适用于: Flow Cyt -
种属反应性
与反应: Rat, Human
预测可用于: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- C6, Hela, SH-SY5Y, HepG2 and fetal brain lysates. Human uterus tissue, HepG2 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8873(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175928于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 113 kDa.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
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| IP |
1/10 - 1/100.
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| 说明 |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 113 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
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IP
1/10 - 1/100. |
靶标
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组织特异性
Widely expressed. -
序列相似性
Contains 1 FERM domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 2037 Human
- Entrez Gene: 13822 Mouse
- Entrez Gene: 309557 Rat
- Omim: 603237 Human
- SwissProt: O43491 Human
- SwissProt: O70318 Mouse
- Unigene: 486470 Human
- Unigene: 306026 Mouse
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别名
- 4.1 G antibody
- 4.1G antibody
- AW555191 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L2 antibody [EPR8873(2)] (ab175928)
Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling EPB41L2 with ab17928 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-EPB41L2 antibody [EPR8873(2)] (ab175928)](https://www.abcam.cn/ps/products/175/ab175928/Images/ab175928-202936-anti-epb4il2-antibody-epr88732-western-blot.jpg)
Western blot - Anti-EPB41L2 antibody [EPR8873(2)] (ab175928)All lanes : Anti-EPB41L2 antibody [EPR8873(2)] (ab175928) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Hela cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 113 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-EPB41L2 antibody [EPR8873(2)] (ab175928)](https://www.abcam.cn/ps/products/175/ab175928/Images/ab175928-202940-anti-epb4il2-antibody-epr88732-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EPB41L2 antibody [EPR8873(2)] (ab175928)Immunofluorescent staining of HepG2 cells using labeling EPB41L2 with ab175928 at 1/100 dilution (green). DAPI nuclear staining (blue).
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![Immunoprecipitation - Anti-EPB41L2 antibody [EPR8873(2)] (ab175928)](https://www.abcam.cn/ps/products/175/ab175928/Images/ab175928-202942-anti-epb4il2-antibody-epr88732-immunoprecipitation.jpg)
Immunoprecipitation - Anti-EPB41L2 antibody [EPR8873(2)] (ab175928)Western blot analysis on immunoprecipitation pellet from fetal brain lysate using ab175928 at 1/10 dilution
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![Anti-EPB41L2 antibody [EPR8873(2)] (ab175928)](https://www.abcam.cn/ps/products/175/ab175928/Images/ab175928-5-benefits-of-recombinant-antibodies.png)
Anti-EPB41L2 antibody [EPR8873(2)] (ab175928)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab175928 被引用在 1 文献中.
- Kaafarani A et al. Proximity Interactome Analysis of Super Conserved Receptors Expressed in the Brain Identifies EPB41L2, SLC3A2, and LRBA as Main Partners. Cells 12:N/A (2023). PubMed: 37998360