Anti-Epac2抗体(ab124189)
Key features and details
- Rabbit polyclonal to Epac2
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Epac2抗体
参阅全部 Epac2 一抗 -
描述
兔多克隆抗体to Epac2 -
宿主
Rabbit -
特异性
At least two isoforms of Epac2 are known to exist; this antibody will detect only the larger isoform. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide corresponding to 15 amino acids near the N-terminus of Human Epac2 (NP_008954).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124189于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 116 kDa.
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| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 116 kDa. |
靶标
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功能
Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2. -
组织特异性
Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels. -
序列相似性
Contains 2 cyclic nucleotide-binding domains.
Contains 1 DEP domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 Ras-GEF domain. -
结构域
The N-terminal nucleotide phosphate binding region cAMP 1 has a much lower affinity for cAMP as compared to cAMP 2.
The DEP domain is involved in membrane localization independent from regulation by cAMP. -
细胞定位
Cytoplasm. Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 252857 Rat
- SwissProt: Q9Z1C7 Rat
- Unigene: 42890 Rat
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别名
- CAMP GEFII antibody
- cAMP regulated guanine nucleotide exchange factor II antibody
- cAMP-GEFII antibody
see all
图片
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Western blot - Anti-Epac2 antibody (ab124189)All lanes : Anti-Epac2 antibody (ab124189) at 1 µg/ml
Lane 1 : Rat liver tissue lysate
Lane 2 : Rat liver tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 116 kDa
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab124189 被引用在 2 文献中.
- Trogden KP et al. Regulation of Glucose-Dependent Golgi-Derived Microtubules by cAMP/EPAC2 Promotes Secretory Vesicle Biogenesis in Pancreatic ß Cells. Curr Biol 29:2339-2350.e5 (2019). PubMed: 31303487
- Wang SY et al. Sex Steroids Influence Brain-Derived Neurotropic Factor Secretion From Human Airway Smooth Muscle Cells. J Cell Physiol 231:1586-92 (2016). PubMed: 26566264
