AB21236

Anti-Epac1抗体

Name: Epac1抗体 (ab21236)| Abcam中文官网
Brand: Abcam Limited
SKU: AB21236
Price: 4843 CNY
Availability: InStock
Rating: 3 (2 reviews)

Anti-Epac1 antibody

(2 Reviews)

(16 Publications)

Rabbit Polyclonal Epac1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human RAPGEF3 aa 550-600.

查看别名

CGEF1, EPAC, EPAC1, RAPGEF3, Rap guanine nucleotide exchange factor 3, Exchange factor directly activated by cAMP 1, Exchange protein directly activated by cAMP 1, Rap1 guanine-nucleotide-exchange factor directly activated by cAMP, cAMP-regulated guanine nucleotide exchange factor I, EPAC 1, cAMP-GEFI

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Epac1 antibody (AB21236)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Epac1 antibody (AB21236)

ICC/IF image of ab21236 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21236, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF

applications

免疫原

Synthetic Peptide within Human RAPGEF3 aa 550-600. The exact immunogen used to generate this antibody is proprietary information.

O95398

特异性

ab21236 does not cross react with Epac2. It has not been tested against endogenous Epac1 but recombinant Epac1.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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产品详情

ab21236 is supplied in an antibody stabilisation buffer. IgG concentration 0.78-0.94mg/ml in antibody stabilisation buffer.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Epac1 also known as Exchange Protein directly Activated by cAMP 1 plays an important role in cellular signaling. It is a guanine nucleotide exchange factor with a molecular mass of about 105 kDa. This protein activates Rap1 and Rap2 small GTPases by promoting the exchange of GDP for GTP. Epac1 is widely expressed in various tissues including the heart brain and immune cells. Its distribution indicates its involvement in multiple physiological processes.

Biological function summary

Epac1 influences cell adhesion proliferation and differentiation. It does not function as a single unit; instead it interacts with other proteins to regulate these cellular processes. Its activity is closely linked with the cAMP signaling pathway where it serves as a mediator of cAMP effects independent of protein kinase A (PKA). The interplay between Epac1 and its associated complexes is essential for proper cellular responses to external stimuli.

Pathways

Epac1 contributes to the cAMP signaling and Ras-related pathways. It functions alongside proteins like Rap1 to relay signals that affect cell adhesion and migration. Within these pathways Epac1 acts as a critical mediator between external signals and intracellular responses. Its interactions with other signaling molecules underline its importance in maintaining cellular functions and responses.

Epac1 has associations with cardiovascular diseases and certain cancers. Its dysregulation can influence heart function linking it with proteins such as PKA and Rap1 in pathological conditions. In cancer altered Epac1 expression may influence tumor growth and metastasis reflecting its involvement with cellular proliferation and migration pathways. Understanding Epac1's role in these conditions can provide insights into potential therapeutic strategies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.

See full target information RAPGEF3

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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 28:1393-1408 PubMed35702948

2022

Unraveling the role of Epac1-SOCS3 signaling in the development of neonatal-CRD-induced visceral hypersensitivity in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Ting Huang,Bin-Bin Chen,Zhi-Jing Song,Hui-Li Tang,Rong Hua,Yong-Mei Zhang

Genetics and molecular biology 44:e20200463 PubMed34807222

2021

Overexpression of RAPGEF3 enhances the therapeutic effect of dezocine in treatment of neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Liu,Li Song,Xiaojun Ma,Yong Liu,Hui Huang,Yongsheng Xu,Wei Yan

Neuropathology and applied neurobiology 47:625-639 PubMed33345400

2021

RAPGEF2 mediates oligomeric Aβ-induced synaptic loss and cognitive dysfunction in the 3xTg-AD mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

You-Na Jang,HoChung Jang,Gyu Hyun Kim,Jeong-Eun Noh,Keun-A Chang,Kea Joo Lee

British journal of pharmacology 177:1589-1608 PubMed31721163

2020

β Adrenoceptor-induced cholinergic inhibition in human and rat urinary bladders involves the exchange protein directly activated by cyclic AMP 1 favoring adenosine release.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Silva,M Teresa Magalhães-Cardoso,Fátima Ferreirinha,Sílvia Moreira,Ana Filipa Costa,Diogo Silva,Cátia Vieira,Miguel Silva-Ramos,Paulo Correia-de-Sá

Thyroid : official journal of the American Thyroid 28:1153-1161 PubMed29882482

2018

Deletion of Rap1b, but not Rap1a or Epac1, Reduces Protein Kinase A-Mediated Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Danielle J Huk,Amruta Ashtekar,Alexa Magner,Krista La Perle,Lawrence S Kirschner

Folia histochemica et cytobiologica 56:21-26 PubMed29528086

2018

Epac1 is involved in cell cycle progression in lung cancer through PKC and Cx43 regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Sun,Dai Wang,Ganghao Ai,Longben Tian,Long Zhao,Renzhen Chen,Kai Wang,Dongbei Guo,Youliang Yao,Wenzhi Liu,XIangyu Kong,Xiaoxuan Chen,Yongxing Zhang

Acta diabetologica 54:581-591 PubMed28353063

2017

The microRNA-7-mediated reduction in EPAC-1 contributes to vascular endothelial permeability and eNOS uncoupling in murine experimental retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Veronica Garcia-Morales,Julian Friedrich,Lysanne M Jorna,Manuel Campos-Toimil,Hans-Peter Hammes,Martina Schmidt,Guido Krenning

BJU international 117:179-91 PubMed25981809

2015

Actions of cAMP on calcium sensitization in human detrusor smooth muscle contraction.

Applications

Unspecified application

Species

Unspecified reactive species

Maya Hayashi,Shunichi Kajioka,Momoe Itsumi,Ryosuke Takahashi,Nouval Shahab,Takao Ishigami,Masahiro Takeda,Noriyuki Masuda,Akito Yamaguchi,Seiji Naito

Toxicologic pathology 42:709-24 PubMed24705881

2014

The role of eNOS phosphorylation in causing drug-induced vascular injury.

Applications

Unspecified application

Species

Unspecified reactive species

Grainne A McMahon Tobin,Jun Zhang,David Goodwin,Sharron Stewart,Lin Xu,Alan Knapton,Carlos González,Simona Bancos,Leshuai Zhang,Michael P Lawton,Bradley E Enerson,James L Weaver

Human molecular genetics 22:4502-15 PubMed23804752

2013

Deficiency of the purine metabolic gene HPRT dysregulates microRNA-17 family cluster and guanine-based cellular functions: a role for EPAC in Lesch-Nyhan syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Ghiabe-Henri Guibinga,Fiona Murray,Nikki Barron,William Pandori,Gorjan Hrustanovic

View all publications

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Anti-Epac1抗体

Anti-Epac1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Epac1 antibody (AB21236)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性