重组Anti-ENTPD5抗体[EPR3784] (ab108603)
![Western blot - Anti-ENTPD5 antibody [EPR3784] (ab108603)](https://www.abcam.cn/ps/products/108/ab108603/Images/ab108603-121320-anti-entpd5-antibody-epr3784-western-blot.gif "Western blot - Anti-ENTPD5 antibody [EPR3784] (ab108603)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3784] to ENTPD5
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-ENTPD5抗体[EPR3784]
参阅全部 ENTPD5 一抗 -
描述
兔单克隆抗体[EPR3784] to ENTPD5 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Fetal liver cell lysate; Human kidney tissue
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR3784 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108603于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa).
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
应用说明
Is unsuitable for Flow Cyt or ICC/IF.
靶标
-
功能
Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. Also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling. -
组织特异性
Expressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues. -
通路
Protein modification; protein glycosylation. -
序列相似性
Belongs to the GDA1/CD39 NTPase family. -
翻译后修饰
N-glycosylated; high-mannose type. -
细胞定位
Endoplasmic reticulum lumen. - Information by UniProt
-
数据库链接
- Entrez Gene: 957 Human
- Entrez Gene: 12499 Mouse
- Entrez Gene: 314312 Rat
- Omim: 603162 Human
- SwissProt: O75356 Human
- SwissProt: Q9WUZ9 Mouse
- SwissProt: Q6P6S9 Rat
- Unigene: 720540 Human
see all -
别名
- CD39 like 4 antibody
- AI196558 antibody
- AI987697 antibody
see all
图片
-
Western blot - Anti-ENTPD5 antibody [EPR3784] (ab108603)Anti-ENTPD5 antibody [EPR3784] (ab108603) at 1/1000 dilution + fetal liver cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENTPD5 antibody [EPR3784] (ab108603)](https://www.abcam.cn/ps/products/108/ab108603/Images/ab108603-121319-anti-entpd5-antibody-epr3784-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENTPD5 antibody [EPR3784] (ab108603)ab108603, at 1/100 dilution, staining ENTPD5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-ENTPD5 antibody [EPR3784] (ab108603)](https://www.abcam.cn/ps/products/108/ab108603/Images/ab108603-3-benefits-of-recombinant-antibodies.png)
Anti-ENTPD5 antibody [EPR3784] (ab108603)
数据表及文件
-
SDS download
-
Datasheet download
文献 (6)
ab108603 被引用在 6 文献中.
- Yu Y et al. PTEN phosphatase inhibits metastasis by negatively regulating the Entpd5/IGF1R pathway through ATF6. iScience 26:106070 (2023). PubMed: 36824269
- Pavlakis E et al. Mutant p53-ENTPD5 control of the calnexin/calreticulin cycle: a druggable target for inhibiting integrin-α5-driven metastasis. J Exp Clin Cancer Res 42:203 (2023). PubMed: 37563605
- Wei J et al. ER-associated ubiquitin ligase HRD1 programs liver metabolism by targeting multiple metabolic enzymes. Nat Commun 9:3659 (2018). PubMed: 30201971
- Shah S et al. Targeting ACLY sensitizes castration-resistant prostate cancer cells to AR antagonism by impinging on an ACLY-AMPK-AR feedback mechanism. Oncotarget 7:43713-43730 (2016). PubMed: 27248322
- Walsh J et al. Identification and quantification of the basal and inducible Nrf2-dependent proteomes in mouse liver: biochemical, pharmacological and toxicological implications. J Proteomics 108:171-87 (2014). WB ; Mouse . PubMed: 24859727
- Curry NL et al. Pten-null tumors cohabiting the same lung display differential AKT activation and sensitivity to dietary restriction. Cancer Discov 3:908-21 (2013). WB, IHC-P ; Mouse . PubMed: 23719831