AB108603

重组Anti-ENTPD5抗体[EPR3784]

Name: ENTPD5抗体[EPR3784] (ab108603)| Abcam中文官网
Brand: Abcam Limited
SKU: AB108603
Price: 2882 CNY
Availability: InStock
Rating: 4 (1 reviews)

Anti-ENTPD5 antibody [EPR3784]

RabMAb
Recombinant
KO Validated
了解详情

(1 Review)

(7 Publications)

Rabbit Recombinant Monoclonal ENTPD5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications.

查看别名

CD39L4, PCPH, ENTPD5, Nucleoside diphosphate phosphatase ENTPD5, CD39 antigen-like 4, ER-UDPase, Ectonucleoside triphosphate diphosphohydrolase 5, Guanosine-diphosphatase ENTPD5, Inosine diphosphate phosphatase ENTPD5, Nucleoside diphosphatase, Uridine-diphosphatase ENTPD5, NTPDase 5, GDPase ENTPD5, UDPase ENTPD5

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENTPD5 antibody [EPR3784] (AB108603)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENTPD5 antibody [EPR3784] (AB108603)

ab108603 at 1/100 dilution staining ENTPD5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ENTPD5 antibody [EPR3784] (AB108603)

All lanes:

Western blot - Anti-ENTPD5 antibody [EPR3784] (ab108603) at 1/1000 dilution

All lanes:

fetal liver cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 48 kDa

Observed band size: 47 kDa

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Lab

Western blot - Anti-ENTPD5 antibody [EPR3784] (AB108603)

Western blot : Rabbit Monoclonal [EPR3784] to ENTPD5 ab108603 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 48 kDa in Wild-type A549 cell lysates with no signal observed at this size in ENTPD5 knockout A549 cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-ENTPD5 antibody [EPR3784] (ab108603) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

ENTPD5 knockout A549 at 20 µg

Lane 3:

HeLa at 20 µg

Lane 4:

THP-1 at 20 µg

Lane 5:

PC-3 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-ENTPD5 antibody [EPR3784] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3784

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Antigen retrieval is recommended.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ENTPD5 also known as ectonucleoside triphosphate diphosphohydrolase 5 or NTPDase-5 primarily acts as an ER-resident glycoprotein that hydrolyzes nucleoside diphosphates into monophosphates. This enzyme weighs approximately 45 kDa. It is predominantly expressed in liver and pancreas tissues. Functionally ENTPD5 regulates cellular levels of UDP by converting GDP and its role in the re-glycosylation of proteins.

Biological function summary

This enzyme plays a role in protein folding and quality control within the endoplasmic reticulum. ENTPD5 influences the ER-associated degradation (ERAD) pathway which deals with the removal of misfolded proteins. The enzyme does interact with other proteins and complexes including calnexin and calreticulin chaperone systems suggesting its integration in glycan-processing machinery.

Pathways

ENTPD5 integrates into the protein glycosylation and turnover pathways. It links with the AKT/mTOR signaling pathway by regulating the availability of UDP-GlcNAc a substrate for N-glycosylation of proteins. ENTPD5 relates to proteins like AKT and mTOR that are involved in protein synthesis cell growth and survival signaling pathways.

ENTPD5 has an association with cancer notably prostate cancer. The enzyme modulates the Warburg effect linked to the increased reglycosylation of proteins promoting tumor progression. It also connects to diabetes through its role in insulin signaling pathways where aberrant glycoprotein fragments may affect insulin receptor activity. Here ENTPD5 shows a connection to proteins involved in insulin signaling such as the insulin receptor.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Hydrolyzes nucleoside diphosphates with a preference for GDP, IDP and UDP compared to ADP and CDP (PubMed : 10400613, PubMed : 15698960). In the lumen of the endoplasmic reticulum, hydrolyzes UDP that acts as an end-product feedback inhibitor of the UDP-Glc : glycoprotein glucosyltransferases. UMP can be transported back by an UDP-sugar antiporter to the cytosol where it is consumed to regenerate UDP-glucose. Therefore, it positively regulates protein reglucosylation by clearing UDP from the ER lumen and by promoting the regeneration of UDP-glucose. Protein reglucosylation is essential to proper glycoprotein folding and quality control in the ER (By similarity).

See full target information ENTPD5

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:11609 PubMed38773214

2024

Uracil as a biomarker for spatial pyrimidine metabolism in the development of gingivobuccal oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Soni Shaikh,Sangramjit Basu,Swarnendu Bag,Ankita Chatterjee,Sourav Datta,Devmalya Banerjee,Kapila Manikantan,Indu Arun,Pattatheyil Arun,Nidhan K Biswas,Arindam Maitra,Deepak Kumar Mishra,Partha P Majumder,Harsh Dhar,Geetashree Mukherjee

Journal of experimental & clinical cancer research : CR 42:203 PubMed37563605

2023

Mutant p53-ENTPD5 control of the calnexin/calreticulin cycle: a druggable target for inhibiting integrin-α5-driven metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Evangelos Pavlakis,Michelle Neumann,Nastasja Merle,Ronja Wieboldt,Michael Wanzel,Viviane Ponath,Elke Pogge von Strandmann,Sabrina Elmshäuser,Thorsten Stiewe

iScience 26:106070 PubMed36824269

2023

PTEN phosphatase inhibits metastasis by negatively regulating the Entpd5/IGF1R pathway through ATF6.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlin Yu,Meng Dai,Liping Huang,Weiping Chen,Ellen Yu,Arnulfo Mendoza,Helen Michael,Chand Khanna,Marcus Bosenberg,Martin McMahon,Glenn Merlino

Nature communications 9:3659 PubMed30201971

2018

ER-associated ubiquitin ligase HRD1 programs liver metabolism by targeting multiple metabolic enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Juncheng Wei,Yanzhi Yuan,Lu Chen,Yuanming Xu,Yuehui Zhang,Yajun Wang,Yanjie Yang,Clara Bien Peek,Lauren Diebold,Yi Yang,Beixue Gao,Chaozhi Jin,Johanna Melo-Cardenas,Navdeep S Chandel,Donna D Zhang,Hui Pan,Kezhong Zhang,Jian Wang,Fuchu He,Deyu Fang

Oncotarget 7:43713-43730 PubMed27248322

2016

Targeting ACLY sensitizes castration-resistant prostate cancer cells to AR antagonism by impinging on an ACLY-AMPK-AR feedback mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Supriya Shah,Whitney J Carriveau,Jinyang Li,Sydney L Campbell,Piotr K Kopinski,Hee-Woong Lim,Natalie Daurio,Sophie Trefely,Kyoung-Jae Won,Douglas C Wallace,Constantinos Koumenis,Anthony Mancuso,Kathryn E Wellen

Journal of proteomics 108:171-87 PubMed24859727

2014

Identification and quantification of the basal and inducible Nrf2-dependent proteomes in mouse liver: biochemical, pharmacological and toxicological implications.

Applications

Species

Mouse

Joanne Walsh,Rosalind E Jenkins,Michael Wong,Adedamola Olayanju,Helen Powell,Ian Copple,Paul M O'Neill,Christopher E P Goldring,Neil R Kitteringham,B Kevin Park

Cancer discovery 3:908-21 PubMed23719831

2013

Pten-null tumors cohabiting the same lung display differential AKT activation and sensitivity to dietary restriction.

Applications

WB, IHC-P

Species

Mouse, Mouse

Natasha L Curry,Mari Mino-Kenudson,Trudy G Oliver,Omer H Yilmaz,Vedat O Yilmaz,Jade Y Moon,Tyler Jacks,David M Sabatini,Nada Y Kalaany

View all publications

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重组Anti-ENTPD5抗体[EPR3784]