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Microbiology Organism Bacteria Gram positive bacteria Other gram +ve bacteria

Anti-Enterococcus抗体(ab19980)

  • Datasheet
  • SDS
Reviews (1)Q&A (9)References (2)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Enterococcus antibody (ab19980)

    Key features and details

    • Rabbit polyclonal to Enterococcus
    • Suitable for: IHC-P, ICC/IF
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Enterococcus抗体
      参阅全部 Enterococcus 一抗
    • 描述

      兔多克隆抗体to Enterococcus
    • 宿主

      Rabbit
    • 经测试应用

      适用于: IHC-P, ICC/IFmore details
    • 种属反应性

      与反应: Enterococcus faecium
    • 免疫原

      Tissue, cells or virus corresponding to Enterococcus. Enterococcus faecium

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • 存储溶液

      Preservative: 0.1% Sodium azide
      Constituent: 0.0268% PBS

      No stabilizing proteins have been added.
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 纯化说明

      >95% pure.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Microbiology
      • Organism
      • Bacteria
      • Gram positive bacteria
      • Other gram +ve bacteria

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab19980于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (1)
    Use at an assay dependent concentration.
    ICC/IF
    1/1000.
    说明
    IHC-P
    Use at an assay dependent concentration.
    ICC/IF
    1/1000.

    靶标

    • 相关性

      Enterococci form part of the lactic acid bacteria family and are the third most important bacterial genus in hospital infections, especially Enterococcus (E.) faecium. In food they can spoil processed meats but they are equally important for ripening and aroma development of certain traditional cheeses and sausages, and are used as human probiotics. However, they possesses a broad spectrum antibiotic resistance which has important implications to human health and disease.
    • 别名

      • E. faecium antibody
      • E.faecalis antibody

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Enterococcus antibody (ab19980)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Enterococcus antibody (ab19980)This image is courtesy of an Abreview submitted by Jan Kučera
      Immunohistochemical analysis of porcine plasma-based in vitro grown biofilm, staining Enterococcus with ab19980.

      Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes a room temperature. Samples were incubated with primary antibody (1/10000 in blocking buffer) for 18 hours at 4°C. Staining was detected using DAB.

      See Abreview

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (2)

    发表研究结果有使用 ab19980?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab19980 被引用在 2 文献中.

    • Cárdenas-Calderón C  et al. Development of an implantable three-dimensional model of a functional pathogenic multispecies biofilm to study infected wounds. Sci Rep 12:21846 (2022). PubMed: 36528648
    • Scotland KB  et al. Uropathogens Preferrentially Interact with Conditioning Film Components on the Surface of Indwelling Ureteral Stents Rather than Stent Material. Pathogens 9:N/A (2020). PubMed: 32962019

    客户评价及客户问答

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    1-10 of 10 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Enterococcus antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (porcine plasma-based in vitro grown biofilm)
    Specification
    porcine plasma-based in vitro grown biofilm
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Jan Kucera

    Verified customer

    提交于 Nov 08 2012

    Question





    One of our customers is looking for an Anti-Enterococcus feacalis Ab, they would prefer that the Ab will be labeled by any florescence dye (FITS for example ).

    Unfortunately I didn't find, is it correct or I missed it?

    We offer them the Ab- Anti-Enterococcus antibody #ab19980 .On the detailed description it is written that the Immunogen is , E.faecalis,and on the alternative names are E. faecium antibody, E.faecalis antibody.

    Base on this is there any information about cross-reactivity of the Ab between the different types?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 19 2014

    Answer


    This antibody will detect the whole cells, and because the antiserum has not been absorbed (only Protein A purified) it may react with related microorganisms.


    As this antibody is suitable for conjugation purpose according to the datasheet, I would like to sduggest your customer is having a look at our FITC conjugation kit.


    Read More

    Abcam Scientific Support

    回复于 Mar 19 2014

    Question

    Thanks a lot

    One quick additional question. I have read that Strep uberis can be picked up by Group B antibodies. What do you say to that?

    Kind Regards

    Read More

    Abcam community

    Verified customer

    Asked on Dec 10 2012

    Answer

    Thank you for contacting us.

    I have attached the list of antibodies we have along with details. Please check.

    I am sorry I am unable to confirm the species cross reactivity of the these antibodies other than waht is given on the datasheet.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    回复于 Dec 10 2012

    Question

    We are developing an assay for agricultural diagnostics and we are interested in following antibodies.
    Staphylococcus aureus,
    Strep. dysgalactiae,
    Strep. uberis,
    Enterococci generic
    Strep agalactiae
    Esherichia coli,
    Klebsiella generic

    I saw your Staphylococcus aureus product online and was wondering if you had more antibodies to offer against all or a subset of the bacteria listed above.

    Kind Regards

    Read More

    Abcam community

    Verified customer

    Asked on Dec 10 2012

    Answer

    Thank you for contacting us.

    ab41181 is anti Strep agalactiae, it will weakly cross react with Strep. dysgalactiae.

    ab41203 is anti Strep agalactiae, it will weakly cross react with Strep. dysgalactiae.

    ab19980 is an anti Enterococcus antibody

    ab68450, ab69185 and ab68451 are anti E. Coli antibodies.

    ab69468, ab20947, ab69467 and ab65926 are the Klebsiella spp specific antibodies.

    We do not have anti Strep. uberis antibodies.

    Please check our new western blot range:

    https://www.abcam.com/index.html?c=5634

    https://www.abcam.com/index.html?pageconfig=productmap&cl=918

    https://www.abcam.com/index.html?pageconfig=resource&rid=12886

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    回复于 Dec 10 2012

    Question

    Could you please advance my Anti-Pseudomonas antibody (ab68538) abreview (no. 32931) to your scientific team?

    I have a problem submitting it again due to „no secondary species“.

    Thanks in advance for your cooperation.

    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Nov 13 2012

    Answer

    Thank you for your enquiry.

    This is to confirm that the status of this Abreview has been changed from edited to submitted. It will be processed shortly by a member of our Scientific Team.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    回复于 Nov 13 2012

    Question

    Thank you for your response.

    Regarding one yet unsubmited abreview (for Anti-Pseudomonas antibody (ab68538): I’m awaiting one more test result tomorrow which will close up the rating...

    Regarding the discount codes I obtained from “AbTrial program” I would like to ask you a question: Are the codes active directly after submitting the abreview? Since my codes expire on Monday, 12/11/2012 is thus the last day I can order new antibodies... Am I right?

    Thank you for response in advance.

    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Nov 09 2012

    Answer

    Thank you for your response.

    This is to confirm that the two other Abreviews have been published and the Discount codes are active. As soon as the third Abreview is submitted, its Discount code will be activated as well.

    Good luck with the experiments and I am looking forward to receiving the Abreview.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    回复于 Nov 09 2012

    Question

    I would like to submit abreviews for below mentioned antibodies, but I have an problem with the abreview form:

    Our laboratory uses the EnVision® + Kit HRP. Rabbit/Mouse (DAB+) as a secondary antibody in the IHC protocol, which is actually a marked polymer and is not in the list of secondary antibodies. Abreview form reports an error and wants to have this field filled for successful abreview.

    How should I proceed?

    I look forward to hearing from you.

    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Nov 07 2012

    Answer

    Thank you for your enquiry.

    I have changed the status from edited to submitted of these two Abreviews (ab19980 and ab20556). They have come through to our internal system without any problem so they will be processed very shortly by one of the members of our Scientific Team.

    However, it seems that the other Abreview ab68538 does not have the “Rating” information and the system recognizes it as a potential problem. I have resent this to you and ask you please look through all the information and resubmit it.

    Thank you for your co-operation and I look forward to receiving your Abreviews.

    Read More

    Abcam Scientific Support

    回复于 Nov 07 2012

    Question

    Thanks a lot for your responce.

    I agree with Terms and Conditions after reading it again at https://www.abcam.com/collaborationdiscount. I hope, that my testing will produce at least satisfactory results.

    Today I asked our Lab-manager to order antibodies, so we can start testing it as soon as possible after delivery.

    I would like to ask you for sending me the below mentioned 3 discount codes which will be used after hopefully succesfull development of the staining procedure and submitting the Abreviews at abcam’s web.

    Thank you again for your interest and time spent during our correspondence.

    I look foreward to hearing from you.

    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 12 2012

    Answer

    DISCOUNT CODES:

    ab19980 - 100%ABR-E76ZR
    ab20556 - 100%ABR-E76ZL
    ab68538 - 100%ABR-E7657




    Expiration date: 12/11/2012

    I am very pleased to hear you would like to accept our offer and testab19980, ab20556 and ab68538in IHC-P.

    This code will give you: 1 per discount code free PRIMARY ANTIBODY OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    回复于 Jul 12 2012

    Question

    I would like to test Anti-Enterococcus antibody (ab19980) in IHC-P application on biofilm and minipig tissue samples. But in the same experiment with same samples, I plan to detect Bacillus bacteria with Anti-Bacillus cereus antibody (ab20556) and Pseudomonas bacteria with Anti-Pseudomonas antibody (ab68538) also which are both not tested in IHC-P application yet. (And the fourth antibody to be ordered is Anti-Staphylococcus aureus antibody [704] (ab 37644) which is already IHC-P aprooved.)

    It is much more simple and less time consuming to test all three mentionned antibodies in the same run in order to develop and implement the optimal staining protocol for our laboratory, but what if I order the antibodies separatelly what is profitable for me if discount will be asigned with each order even over paying the shipping 3 times?

    I’ll be greatfull, If I could get a discount for all Abs what you probably understand.

    It depends on what is more valuable for your company, whether money or application information.

    Thanks for an answer in advance.



    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Jul 10 2012

    Answer

    Thank you for your response and your interest.

    It is very unusual to offer a discount code for more than 1 product particularly when the application is not one of the standard immunoassays (Western blot, ELISA, Flow cytometry, Immunohistochemistry etc).

    The technique you wish to use/test our products is not the classical IHC-P (formalin-fixed paraffin embedded immunohistochemistry) procedure. At this stage it is not know how frequently other customers will use these products in such an assay and how easy to modify the technique.

    Could you please confirm how the tissue sections will be processed and fixed and whether you have any publication on this specific technique? We need to consider carefully the applicability of this assay.

    I look forward to hearing from you soon.

    Read More

    Abcam Scientific Support

    回复于 Jul 10 2012

    Question

    I would like to enquire as to whether this antibody will react with Enterococcus faecalis ATCC 51299. Thank you for your time.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 13 2006

    Answer

    I now have more details on experiments done on the antibody ab19980. This antibody has been tested by us only against the immunogen species E. faecium. To the best of my knowledge, I know that there have been experiments done externally that were able to detect E. faecalis, however I do not have access to the assay details. With thanks,

    Read More

    Abcam Scientific Support

    回复于 Jul 14 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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