重组Anti-ENSA抗体[EPR8008(2)] (ab180513)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8008(2)] to ENSA
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-ENSA抗体[EPR8008(2)]
参阅全部 ENSA 一抗 -
描述
兔单克隆抗体[EPR8008(2)] to ENSA -
宿主
Rabbit -
特异性
ENSA (O43768) and ARPP-19 (P56211) -
经测试应用
适用于: Flow Cyt (Intra), WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- K562 cells, HeLa whole cell lysate (ab150035), Fetal brain, Rat and Mouse heart tissue lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8008(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180513于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/70.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
|
说明 |
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Flow Cyt (Intra)
1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa. |
靶标
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功能
Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents. -
组织特异性
Widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. -
序列相似性
Belongs to the endosulfine family. -
翻译后修饰
Phosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A (By similarity). Phosphorylated by PKA. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 2029 Human
- Entrez Gene: 56205 Mouse
- Entrez Gene: 60334 Rat
- Omim: 603061 Human
- SwissProt: O43768 Human
- SwissProt: P60840 Mouse
- SwissProt: P60841 Rat
- Unigene: 632456 Human
see all -
别名
- Alpha endosulfine antibody
- Alpha-endosulfine antibody
- ARPP 19e antibody
see all
图片
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All lanes : Anti-ENSA antibody [EPR8008(2)] (ab180513) at 1/10000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Mouse heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution
Predicted band size: 13 kDaBlocking/dilutin buffer 5% NFDM/TBST
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Anti-ENSA antibody [EPR8008(2)] (ab180513) at 1/2000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated Secondary antibody at 1/1000 dilution
Predicted band size: 13 kDaBlocking/diuting buffer buffer 5% NFDM/TBST
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Intracellular flow cytometric analysis of K562 cells labeling ENSAwith ab180513 at 1/70 dilution. Goat anti rabbit IgG (FITC) at 1/75 dilution was used as the secondary antibody. Cells were fixed in 2% paraformaldehyde.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab180513 被引用在 1 文献中.
- Gouttia OG et al. The MASTL-ENSA-PP2A/B55 axis modulates cisplatin resistance in oral squamous cell carcinoma. Front Cell Dev Biol 10:904719 (2022). PubMed: 36247015