Anti-eNOS (phospho S632)抗体[M232] (ab76199)
![Western blot - Anti-eNOS (phospho S632) antibody [M232] (ab76199)](https://www.abcam.cn/ps/products/76/ab76199/Images/ab76199-91558-anti-enos-phospho-s632-antibody-m232-western-blot.gif "Western blot - Anti-eNOS (phospho S632) antibody [M232] (ab76199)"){kind=small}
Key features and details
- Mouse monoclonal [M232] to eNOS (phospho S632)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-eNOS (phospho S632)抗体[M232]
参阅全部 eNOS 一抗 -
描述
小鼠单克隆抗体[M232] to eNOS (phospho S632) -
宿主
Mouse -
特异性
ab76199 is not expected to react with other NOS family members due to low homology. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to Mouse eNOS (phospho S632).
Database link: P70313 -
阳性对照
- Human umbilical vein endothelial cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
M232 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76199于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500. Detects a band of approximately 140, 120 kDa (predicted molecular weight: 133 kDa).
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| 说明 |
|---|
|
WB
1/500. Detects a band of approximately 140, 120 kDa (predicted molecular weight: 133 kDa). |
靶标
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功能
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. -
组织特异性
Platelets, placenta, liver and kidney. -
疾病相关
Variation in NOS3 seem to be associated with susceptibility to coronary spasm. -
序列相似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. -
翻译后修饰
Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. -
细胞定位
Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity. - Information by UniProt
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数据库链接
- Entrez Gene: 4846 Human
- Entrez Gene: 24600 Rat
- Omim: 163729 Human
- SwissProt: P29474 Human
- SwissProt: Q62600 Rat
- Unigene: 647092 Human
- Unigene: 44265 Rat
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别名
- cNOS antibody
- Constitutive NOS antibody
- EC NOS antibody
see all
图片
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Western blot - Anti-eNOS (phospho S632) antibody [M232] (ab76199)All lanes : Anti-eNOS (phospho S632) antibody [M232] (ab76199) at 1/500 dilution
Lane 1 : human umbilical vein endothelial
cells, untreated
Lane 2 : human umbilical vein endothelial
cells, treated with lambda phosphatase
Predicted band size: 133 kDa
Observed band size: 120,140 kDa why is the actual band size different from the predicted?
The 120 kDa band may be a truncated form of eNOS.
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab76199 被引用在 15 文献中.
- Guan Y & Wang X Salvianic Acid A Regulates High-Glucose-Treated Endothelial Progenitor Cell Dysfunction via the AKT/Endothelial Nitric Oxide Synthase (eNOS) Pathway. Med Sci Monit 27:e928153 (2021). PubMed: 33770068
- Cheng X et al. Inhibition of LOXL1-AS1 alleviates oxidative low-density lipoprotein induced angiogenesis via downregulation of miR-590-5p mediated KLF6/VEGF signaling pathway. Cell Cycle 20:1663-1680 (2021). PubMed: 34334119
- Ye P et al. Endothelial Agrin Is Dispensable for Normal and Tumor Angiogenesis. Front Cardiovasc Med 8:810477 (2021). PubMed: 35174224
- Xu J et al. Knockdown of Long Noncoding RNA (lncRNA) AK094457 Relieved Angiotensin II Induced Vascular Endothelial Cell Injury. Med Sci Monit 26:e919854 (2020). PubMed: 32027625
- Xu H et al. GATA4 protects against hyperglycemia-induced endothelial dysfunction by regulating NOX4 transcription. Mol Med Rep 17:1485-1492 (2018). PubMed: 29138836