Anti-eNOS抗体[M221] (ab76198)
![Western blot - Anti-eNOS antibody [M221] (ab76198)](https://www.abcam.cn/ps/products/76/ab76198/Images/ab76198-254561-anti-enos-antibody-m221-western-blot.jpg "Western blot - Anti-eNOS antibody [M221] (ab76198)")
Key features and details
- Mouse monoclonal [M221] to eNOS
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-eNOS抗体[M221]
参阅全部 eNOS 一抗 -
描述
小鼠单克隆抗体[M221] to eNOS -
宿主
Mouse -
特异性
ab76198 is not predicted to react with other NOS family members due to low homology.
This antibody detects eNOS in mouse and but at a lower intensity than in human. If you are working in mouse, we would recommend using no more than 1% milk as the blocking agent for optimal signal strength.
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经测试应用
适用于: IHC-P, ICC/IF, WBmore details
不适用于: Flow Cyt (Intra) -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Mouse eNOS aa 1024-1202 (C terminal).
Database link: P70313 -
阳性对照
- Human umbilical vein endothelial cells untreated and treated with lambda phosphatase. Mouse placenta lysate. Huvec lysate. IHC-P: FFPE human normal placenta tissue sections.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
M221 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76198于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| WB | (11) |
1/500 - 1/1000. Predicted molecular weight: 133 kDa.
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| 说明 |
|---|
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Predicted molecular weight: 133 kDa. |
靶标
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功能
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. -
组织特异性
Platelets, placenta, liver and kidney. -
疾病相关
Variation in NOS3 seem to be associated with susceptibility to coronary spasm. -
序列相似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. -
翻译后修饰
Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. -
细胞定位
Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity. - Information by UniProt
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数据库链接
- Entrez Gene: 4846 Human
- Entrez Gene: 18127 Mouse
- Omim: 163729 Human
- SwissProt: P29474 Human
- SwissProt: P70313 Mouse
- Unigene: 647092 Human
- Unigene: 258415 Mouse
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别名
- cNOS antibody
- Constitutive NOS antibody
- EC NOS antibody
see all
图片
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Western blot - Anti-eNOS antibody [M221] (ab76198)All lanes : Anti-eNOS antibody [M221] (ab76198) at 1/500 dilution
Lane 1 : Huvec cell lysates
Lane 2 : Mouse placenta lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti Mouse IR680 at 1/10000 dilution
Predicted band size: 133 kDaThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab76198 overnight at 4°C. Antibody binding was detected using Goat anti Mouse IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [M221] (ab76198)](https://www.abcam.cn/ps/products/76/ab76198/Images/ab76198-251250-anti-enos-antibody-m221-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [M221] (ab76198)IHC image of eNOS staining in human normal placenta*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76198, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Immunocytochemistry/ Immunofluorescence - Anti-eNOS antibody [M221] (ab76198)](https://www.abcam.cn/ps/products/76/ab76198/Images/ab76198-269542-anti-enos-antibody-m221-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-eNOS antibody [M221] (ab76198)ab76198 staining eNOS in human umbilical vein endothelial cells. Cells with fixed with paraformaldehyde.
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![Western blot - Anti-eNOS antibody [M221] (ab76198)](https://www.abcam.cn/ps/products/76/ab76198/Images/ab76198-91557-anti-enos-antibody-m221-western-blot.gif)
Western blot - Anti-eNOS antibody [M221] (ab76198)All lanes : Anti-eNOS antibody [M221] (ab76198) at 1/1000 dilution
Lane 1 : human umbilical vein endothelial
cells, untreated
Lane 2 : human umbilical vein endothelial
cells, treated with lambda phosphatase
Predicted band size: 133 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (170)
ab76198 被引用在 170 文献中.
- Su Y et al. Blending with transition metals improves bioresorbable zinc as better medical implants. Bioact Mater 20:243-258 (2023). PubMed: 35702610
- Asif H et al. Title: β3 Adrenergic Receptor Signaling in the Human Myometrium. Reprod Sci 30:124-134 (2023). PubMed: 35380411
- He H et al. Activating NO-sGC crosstalk in the mouse vascular niche promotes vascular integrity and mitigates acute lung injury. J Exp Med 220:N/A (2023). PubMed: 36350314
- Wang W et al. Effect of electroacupuncture on haemodynamic changes during intubation for general anaesthesia is mediated by nitric oxide synthase‑3 via the regulation of microRNA‑155, microRNA‑335 and microRNA‑383. Mol Med Rep 27:N/A (2023). PubMed: 36866732
- Supriya S et al. Effects of polyphenols in non-centrifugal cane sugar on saliva secretion: in vitro and in vivo experiments and a randomized controlled trial. J Clin Biochem Nutr 72:171-182 (2023). PubMed: 36936876