AB199956

Anti-eNOS 抗体 [EPR19296]

Name: eNOS抗体[EPR19296] (ab199956)| Abcam中文官网
Brand: Abcam Limited
SKU: AB199956
Price: 1680 CNY
Availability: InStock
Rating: 4 (3 reviews)

Anti-eNOS antibody [EPR19296]

RabMAb
Recombinant
了解详情

(3 Reviews)

(58 Publications)

Rabbit Recombinant Monoclonal eNOS antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 58 publications.

查看别名

Nitric oxide synthase 3, Constitutive NOS, EC-NOS, NOS type III, cNOS, NOSIII, Endothelial NOS, eNOS, NOS3

6 Images

Supplier Data

Immunoprecipitation - Anti-eNOS antibody [EPR19296] (AB199956)

eNOS was immunoprecipitated from 1 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab199956 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab199956 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HepG2 whole cell lysate 10 μg (Input).

Lane 2 : ab199956 IP in HepG2 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199956 in HepG2 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-eNOS antibody [EPR19296] (ab199956)

Predicted band size: 133 kDa

false

Supplier Data

Western blot - Anti-eNOS antibody [EPR19296] (AB199956)

Blocking/Dilution buffer : 5% NFDM/TBST.

eNOS is shown to be expressed in platelets, placenta, liver and kidney (UniProt entry P29474). It also plays an important role in the cardiovascular system. (PMID : 15210449 & 15936740).

All lanes:

Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 20 µg

Lane 2:

Human fetal kidney lysate at 20 µg

Lane 3:

Human fetal liver lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 133 kDa

Observed band size: 140 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-eNOS antibody [EPR19296] (AB199956)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 17855661 & 11004215).

All lanes:

Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/5000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Whole cell lysate from HepG2 cells treated with 10 μg/ml LPS for 2 hours at 20 µg

Lane 3:

Whole cell lysate from HepG2 cells treated with 10 μg/ml LPS for 6 hours at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 133 kDa

Observed band size: 140 kDa

false

Exposure time: 15s

Lab

Western blot - Anti-eNOS antibody [EPR19296] (AB199956)

Exposure Time : Lane1 : 40 seconds; Lane2 : 180 seconds

The expression profile observed in HeLa is consistent with the literature (PMID : 19925457).
Negative control : HeLa (PMID : 19925457)

All lanes:

Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 133 kDa

Observed band size: 140 kDa

false

Supplier Data

Western blot - Anti-eNOS antibody [EPR19296] (AB199956)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1, 2, 3, 4, 6 and 7 : 30 seconds; Lane 5 : 2 minutes.

eNOS is shown to be expressed in platelets, placenta, liver and kidney (UniProt entry P29474). It also plays an important role in the cardiovascular system. (PMID : 15210449 & 15936740).

All lanes:

Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

Mouse spleen lysate at 10 µg

Lane 5:

Rat brain lysate at 10 µg

Lane 6:

Rat heart lysate at 10 µg

Lane 7:

Rat spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 133 kDa

Observed band size: 140 kDa

false

Supplier Data

Western blot - Anti-eNOS antibody [EPR19296] (AB199956)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 1 second; Lane 2 : 3 minutes.

All lanes:

Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human eNOS protein (His-DDDDK tag N-Terminus) (<a href='/products/proteins-peptides/recombinant-human-enos-protein-tagged-ab198066'>ab198066</a>) at 0.01 µg

Lane 2:

Western blot - Recombinant Human iNOS protein (His-DDDDK tag N-Terminus) (<a href='/products/proteins-peptides/recombinant-human-inos-protein-ab135010'>ab135010</a>) at 0.05 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 133 kDa

Observed band size: 140 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-eNOS antibody [EPR19296] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19296

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "We recommend using freshly made lysate in western blot testing to get stronger band and avoid high background." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "We recommend using freshly made lysate in western blot testing to get stronger band and avoid high background." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "We recommend using freshly made lysate in western blot testing to get stronger band and avoid high background." } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ENOS also known as endothelial nitric oxide synthase is an enzyme important for the production of nitric oxide (NO) in blood vessels. This protein with a molecular weight of approximately 133 kDa is expressed mostly in endothelial cells. eNOS plays a mechanical role in synthesizing NO from L-arginine a process requiring cofactors such as NADPH and oxygen. The activity of eNOS can be investigated through techniques such as Western blot with specific assays like phospho-eNOS ELISA available to measure its phosphorylated forms indicating activated states of the enzyme.

Biological function summary

ENOS contributes significantly to the regulation of vascular tone and blood flow by promoting vasodilation. It does not function alone; instead it forms complexes with other proteins to exert its full effect. eNOS activity influences the process of angiogenesis inflammation modulation and platelet aggregation. Through its ability to produce nitric oxide eNOS acts as a signaling molecule helping maintain vascular homeostasis.

Pathways

ENOS is a critical player in the NO signaling pathway and interacts intricately with the PI3K/Akt pathway. Nitric oxide produced by eNOS has a role in signaling cascades that lead to vascular dilation and reduced blood pressure. The PI3K/Akt pathway regulates eNOS activity via phosphorylation enhancing NO production. Other proteins like caveolin-1 and calmodulin modulate eNOS impacting these pathways' outcomes.

ENOS is associated with cardiovascular conditions like atherosclerosis and hypertension. Dysfunction of eNOS can lead to reduced NO production impairing vasodilation and contributing to these diseases. In cardiovascular disorders eNOS interacts with proteins such as the angiotensin II type 1 receptor which can negatively impact its function exacerbating disease states. Investigating eNOS and its related proteins provides insight into potential therapeutic targets for improving cardiovascular health.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed : 1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.. Isoform eNOS13C. Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

See full target information NOS3

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文献 (58)

Recent publications for all applications. Explore the full list and refine your search

World journal of diabetes 16:109553 PubMed40980297

2025

Farrerol ameliorates diabetic cardiomyopathy by inhibiting ferroptosis miR-29b-3p/SIRT1 signaling pathway in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Guo,Xin-Rou Yu,Hao-Di Gu,Yu-Jie Wang,Zhen-Gang Yang,Ju-Fang Chi,Liu-Ping Zhang,Hui Lin

Kidney & blood pressure research 50:442-459 PubMed40451155

2025

Endothelial MicroRNA-214 Confers Angiotensin II Hypertension by Targeting eNOS in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shuzhen Li,Bing Liu,Shuang Kang,Bingyu Yang,Yue Zhang,Songming Huang,Aihua Zhang,Zhanjun Jia

International journal of molecular sciences 26: PubMed40332770

2025

Rehmanniae Radix Praeparata in Blood Deficiency Syndrome: UPLC-Q-TOF-MS Profiling, Network Pharmacology, and PI3K-AKT Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ruxi Gao,Fanyi Wang,Xiang Liu,Chu Yuan,Guoshun Shan

Journal of molecular histology 56:103 PubMed40063284

2025

Protective role of Tongxinluo in mitigating myocardial fibrosis in mice with acute myocardial infarction via neuregulin-1 upregulation and Inhibition of endothelium-interstitial transition.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Li,Yu-Jie Yin,Ya-Ru Wei,Yi Liu,Ning-Xin Han,Xiao-Qi Wang,Yuan-Jie Hao,Ya-Fen Wang,Yun-Long Hou,Zhen-Hua Jia

Antioxidants (Basel, Switzerland) 13: PubMed39334728

2024

Aerobic Exercise Protects against Cardiotoxin-Induced Skeletal Muscle Injury in a DDAH1-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Feng,Kai Luo,Xinyi Yuan,Ting Lan,Siyu Wang,Xin Xu,Zhongbing Lu

Investigative ophthalmology & visual science 65:34 PubMed39186263

2024

Agonism of β3-Adrenoceptors Inhibits Pathological Retinal Angiogenesis in the Model of Oxygen-Induced Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto Melecchi,Alessio Canovai,Rosario Amato,Massimo Dal Monte,Luca Filippi,Paola Bagnoli,Maurizio Cammalleri

Experimental and therapeutic medicine 28:400 PubMed39171145

2024

GAB1 attenuates lipopolysaccharide‑mediated endothelial dysfunction via regulation of SOCS3.

Applications

Unspecified application

Species

Unspecified reactive species

Guangdong Ren,Ran Liu,Huiqiang Mai,Gang Yin,Fulai Ding,Chunmei Wang,Shuxin Chen,Xianqi Lan

Journal of ophthalmology 2024:7195550 PubMed39049847

2024

ABCA1 Deletion Does Not Affect Aqueous Humor Outflow Function in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Qin,Chunchun Hu,Youjia Zhang,Yuhong Chen,Yuan Lei

Regenerative therapy 27:398-407 PubMed38694446

2024

Adipose-derived stem cells ameliorate radiation-induced lung injury by activating the DDAH1/ADMA/eNOS signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Quanwei Fu,Qiaohui Gao,Shengyuan Jiao,Fei Da,Juan Guo,Yunen Liu,Junye Liu

Experimental and therapeutic medicine 27:183 PubMed38515647

2024

Early postnatal moderate catch‑up growth in rats with nutritional intrauterine growth restriction preserves pulmonary vascular and cognitive function in adulthood.

Applications

Unspecified application

Species

Unspecified reactive species

Lixia Ye,Yajie Huang,Kewei Chen,Chengcheng Hang,Yuhan Ying,Lu Zu,Xiaofei Luo,Lizhong Du

View all publications

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