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Neuroscience Neurotransmission Nitric Oxide NOS

Anti-eNOS抗体(ab5589)

  • Datasheet
  • SDS
Reviews (8)Q&A (26)References (123)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (ab5589)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (ab5589)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (ab5589)

Key features and details

  • Rabbit polyclonal to eNOS
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-eNOS antibody [EPR19296] (ab199956)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-eNOS抗体
    参阅全部 eNOS 一抗
  • 描述

    兔多克隆抗体to eNOS
  • 宿主

    Rabbit
  • 特异性

    This antibody does not detect human inducible NOS or rat brain nNOS. ab5589 has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects an ~140 kDa protein representing eNOS from human endothelial cells. Immunohistochemical staining of eNOS in Human vascular endothelial cells with ab5589 results in prominent staining of the epithelial cells.
  • 经测试应用

    适用于: IHC-Pmore details
    不适用于: WB
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human eNOS aa 1179-1194.
    Sequence:

    SLQERQLRGAVPWAFD

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • IHC-P: Human lung, placenta and spleen tissues.
  • 常规说明

    We have received mixed feedback from customers regarding rat specificity; this is why we are temporarily removing Rat from tested species list and no longer guarantee it. Our laboratory is currently testing new lots so please contact our scientific support team (technical@abcam.com) for more information.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Nitric Oxide
    • NOS
    • Cardiovascular
    • Blood
    • Platelets
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cardiovascular
    • Vasculature
    • Vasodilation
    • Nitric oxide associated
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human eNOS protein (ab112329)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5589于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/100. Antigen retrieval is not essential but may optimise staining.
说明
IHC-P
1/100. Antigen retrieval is not essential but may optimise staining.
应用说明
Is unsuitable for WB.

靶标

  • 功能

    Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
    Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
  • 组织特异性

    Platelets, placenta, liver and kidney.
  • 疾病相关

    Variation in NOS3 seem to be associated with susceptibility to coronary spasm.
  • 序列相似性

    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • 翻译后修饰

    Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
  • 细胞定位

    Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
  • Target information above from: UniProt accession P29474 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4846 Human
    • Entrez Gene: 18127 Mouse
    • Entrez Gene: 24600 Rat
    • Omim: 163729 Human
    • SwissProt: P29474 Human
    • SwissProt: P70313 Mouse
    • SwissProt: Q62600 Rat
    • Unigene: 647092 Human
    • Unigene: 258415 Mouse
    • Unigene: 44265 Rat
    see all
  • 别名

    • cNOS antibody
    • Constitutive NOS antibody
    • EC NOS antibody
    • EC-NOS antibody
    • ecNOS antibody
    • Endothelial nitric oxidase synthase antibody
    • Endothelial nitric oxide synthase antibody
    • Endothelial nitric oxide synthase 3 antibody
    • Endothelial NOS antibody
    • eNOS antibody
    • Nitric oxide synthase 3 (endothelial cell) antibody
    • Nitric oxide synthase 3 antibody
    • Nitric oxide synthase 3 endothelial cell antibody
    • Nitric oxide synthase endothelial antibody
    • Nitric oxide synthase, endothelial antibody
    • NOS 3 antibody
    • NOS III antibody
    • NOS type III antibody
    • NOS3 antibody
    • NOS3_HUMAN antibody
    • NOSIII antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (ab5589)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (ab5589)

    Immunolocalization of eNOS in human lung tissue using ab5589.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (ab5589)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (ab5589)

    IHC image of eNOS staining in human placenta formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5589, 1/100, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (ab5589)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (ab5589)

    The specificity of ab5589 was demonstrated using naturally occurring variable expression to confirm specificity. Immunohistochemistry analysis of human tissue sections using this antibody showed eNOS is strongly stained in human spleen tissue and no staining in human tonsil tissue which does not express eNOS. 

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (123)

发表研究结果有使用 ab5589?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab5589 被引用在 123 文献中.

  • Li J  et al. Radial Sponges Facilitate Wound Healing by Promoting Cell Migration and Angiogenesis. Adv Healthc Mater 12:e2202737 (2023). PubMed: 36603134
  • Feng J  et al. Human umbilical cord mesenchymal stem cells-derived exosomal circDLGAP4 promotes angiogenesis after cerebral ischemia-reperfusion injury by regulating miR-320/KLF5 axis. FASEB J 37:e22733 (2023). PubMed: 36723877
  • Moon HW  et al. Erectile Dysfunction Treatment Using Stem Cell Delivery Patch in a Cavernous Nerve Injury Rat Model. Bioengineering (Basel) 10:N/A (2023). PubMed: 37370566
  • Fu J  et al. Polydopamine (PDA) coatings with endothelial vascular growth factor (VEGF) immobilization inhibiting neointimal formation post zinc (zn) wire implantation in rat aortas. Biomater Res 27:84 (2023). PubMed: 37667399
  • Szekeres R  et al. Therapeutic Aspects of Prunus cerasus Extract in a Rabbit Model of Atherosclerosis-Associated Diastolic Dysfunction. Int J Mol Sci 24:N/A (2023). PubMed: 37686067
View all Publications for this product

客户评价及客户问答

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1-10 of 34 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-eNOS antibody

Good
Abreviews
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Sheep Tissue sections (carotid artery)
Permeabilization
Yes - PBS-tween
Specification
carotid artery
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 20 2019

Western blot abreview for Anti-eNOS antibody

Good
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Western blot
Sample
Cow Cell lysate - whole cell (Aortic Endothelial Cells)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
10 µg
Specification
Aortic Endothelial Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 19 2017

Western blot abreview for Anti-eNOS antibody

Average
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Western blot
Sample
Pig Cell lysate - whole cell (endothelial cell)
Gel Running Conditions
Non-reduced Denaturing (8% gel)
Loading amount
30 µg
Specification
endothelial cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 24 2015

Western blot abreview for Anti-eNOS antibody

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Non-reduced Denaturing (7.5%)
Sample
Human Cell lysate - whole cell (endothelial cells)
Specification
endothelial cells
Treatment
vegf stimulated
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Vidhya Ramaswamy

Verified customer

提交于 Sep 30 2014

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-eNOS antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Mesenteric venule)
Specification
Mesenteric venule
Permeabilization
Yes - Triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Sulei Xu

Verified customer

提交于 Nov 13 2013

Western blot abreview for Anti-eNOS antibody

Good
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (lung endothelial cells)
Gel Running Conditions
Reduced Denaturing (gradient gel)
Loading amount
10 µg
Specification
lung endothelial cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 12 2013

Question

We want to perform western blotting on HUVECs (Human Umbilical Endothelial Vein Cells) and A7R5 cells lines (rat aortic smooth muscle cell), both of the cell lines contain eNOS. As we found various sources of anti-eNOS antibody for WB on Abcam webpage, such as ab66127, ab5589 and ab51086, we are wondering which one could be suitable for our needs?

Read More

Abcam community

Verified customer

Asked on Mar 28 2013

Answer

Any of the following antibodies would be suitable as they are guaranteed to work in human, rat and in WB:

Anti-eNOS antibody (ab66127)
Anti-eNOS antibody (ab5589)
Anti-eNOS antibody (ab51086)
Anti-eNOS antibody [NOS-E1] (ab50010)

Out of these antibodies, I would suggest using ab5589 as we have had one customer who used this in western blot and obtained good results. In addition, this antibody has also been extensively used in WB in several publications.

Read More

Abcam Scientific Support

回复于 Mar 28 2013

Question

Band around 25-30 kDa in Western blot instead of around 130-140 kDa as expected.

Read More

Abcam community

Verified customer

Asked on Nov 07 2012

Answer

Thank you very much for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge vial of ab66217 which should arrive tomorrow.

Please keep me updated about the results after trying this antibody, and let me know if there is anything else that we can do for you. I'll be happy to help.

Read More

Abcam Scientific Support

回复于 Nov 07 2012

Question

Looking for antibodies to use in ICC with human cells.

Read More

Abcam community

Verified customer

Asked on Jul 16 2012

Answer

Thank you so much for your calls today and for your questions.

Please let me know if you would like to try any of the antibodies in ICC that haven't already been tested, and I will be happy to set up the testing discount program for you. If you have any questions or need anything else, please don't hesitate to ask.

Have a great day!

Read More

Abcam Scientific Support

回复于 Jul 16 2012

Question

phone call: searching antibody for eNOS and phospho eNOS on rat and mouse tissue, on IHC-P and WB.

Read More

Abcam community

Verified customer

Asked on Jul 10 2012

Answer

A while ago we spoke in regards to searching for the best antibodies to use for detection of eNOS and phospho eNOS on mouse and rat tissue, on paraffin sections (IHC-P)as well as in Western blot.
..

We have the ab66127 or the ab5589 against total eNOS which have been tested in mouse and rat, as well as in IHC-P and Western blot. These antibodies are therefore guaranteed by us for this use.
https://www.abcam.com/eNOS-antibody-ab66127.html (or use the following: https://www.abcam.com/eNOS-antibody-ab66127.html).
https://www.abcam.com/eNOS-antibody-ab5589.html (or use the following: https://www.abcam.com/eNOS-antibody-ab5589.html).

As an antibody against phsopho eNOS, we do have the ab75639 which recognizes the S1177 on eNOS. This antibody is again tested and guaranteed in mouse, rat and IHC-P and Western blot.
https://www.abcam.com/eNOS-phospho-S1177-antibody-ab75639.html (or use the following: https://www.abcam.com/eNOS-phospho-S1177-antibody-ab75639.html).

I have followed up with the laboratory in regards to the protocols used for the ab75639. For the Western blot, the cell lysates have been prepared with protease and phosphatase inhibitors. Before loading, the lysates have been denatured and reduced. Importantly, the membranes were blocked with 5% BSA in TBS-T. The IHC-P has been performed with heat induced antigen retrieval in Citrate buffer pH 6.

I hope this information is still useful. Please do not hesitate to let me know if you have any question or if you require any other information from our side in regards to these or other antibodies.

Read More

Abcam Scientific Support

回复于 Jul 10 2012

1-10 of 34 Abreviews or Q&A

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