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AB5589

Anti-eNOS 抗体

Anti-eNOS antibody

4

(8 Reviews)

|

(148 Publications)

Anti-eNOS antibody (ab5589) is a rabbit polyclonal antibody detecting eNOS in IHC-P. Suitable for Human, Mouse, Rat.

- Over 120 publications
- Trusted since 2003

查看别名

Nitric oxide synthase 3, Constitutive NOS, EC-NOS, NOS type III, cNOS, NOSIII, Endothelial NOS, eNOS, NOS3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (AB5589)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (AB5589)

IHC image of eNOS staining in human placenta formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5589, 1/100, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (AB5589)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (AB5589)

The specificity of ab5589 was demonstrated using naturally occurring variable expression to confirm specificity. Immunohistochemistry analysis of human tissue sections using this antibody showed eNOS is strongly stained in human spleen tissue and no staining in human tonsil tissue which does not express eNOS.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (AB5589)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (AB5589)

Immunolocalization of eNOS in human lung tissue using ab5589.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

IHC-P

applications

免疫原

Synthetic Peptide within Human NOS3 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P29474

特异性

This antibody does not detect human inducible NOS or rat brain nNOS. ab5589 has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects an ~140 kDa protein representing eNOS from human endothelial cells. Immunohistochemical staining of eNOS in Human vascular endothelial cells with ab5589 results in prominent staining of the epithelial cells.

反应性数据

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产品详情

What is this antibody validated in?
Anti-eNOS antibody (ab5589) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

Trusted by the scientific community
Anti-eNOS (ab5589) was first used in a scientific publication in 2003 and has been cited over 120 times in peer-reviewed journals.

Reviewed by scientists
Anti-eNOS (ab5589) has over 5 independent reviews from customers.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ENOS also known as endothelial nitric oxide synthase is an enzyme important for the production of nitric oxide (NO) in blood vessels. This protein with a molecular weight of approximately 133 kDa is expressed mostly in endothelial cells. eNOS plays a mechanical role in synthesizing NO from L-arginine a process requiring cofactors such as NADPH and oxygen. The activity of eNOS can be investigated through techniques such as Western blot with specific assays like phospho-eNOS ELISA available to measure its phosphorylated forms indicating activated states of the enzyme.
Biological function summary

ENOS contributes significantly to the regulation of vascular tone and blood flow by promoting vasodilation. It does not function alone; instead it forms complexes with other proteins to exert its full effect. eNOS activity influences the process of angiogenesis inflammation modulation and platelet aggregation. Through its ability to produce nitric oxide eNOS acts as a signaling molecule helping maintain vascular homeostasis.

Pathways

ENOS is a critical player in the NO signaling pathway and interacts intricately with the PI3K/Akt pathway. Nitric oxide produced by eNOS has a role in signaling cascades that lead to vascular dilation and reduced blood pressure. The PI3K/Akt pathway regulates eNOS activity via phosphorylation enhancing NO production. Other proteins like caveolin-1 and calmodulin modulate eNOS impacting these pathways' outcomes.

ENOS is associated with cardiovascular conditions like atherosclerosis and hypertension. Dysfunction of eNOS can lead to reduced NO production impairing vasodilation and contributing to these diseases. In cardiovascular disorders eNOS interacts with proteins such as the angiotensin II type 1 receptor which can negatively impact its function exacerbating disease states. Investigating eNOS and its related proteins provides insight into potential therapeutic targets for improving cardiovascular health.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed : 1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.. Isoform eNOS13C. Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
See full target information NOS3

文献 (148)

Recent publications for all applications. Explore the full list and refine your search

Pharmacology research & perspectives 13:e70167 PubMed40842442

2025

6-Nitrodopamine Release From Mouse Seminal Vesicles Is Dependent on Endothelial Nitric Oxide Synthase (eNOS) Activation.

Applications

Unspecified application

Species

Unspecified reactive species

José Britto-Júnior,Pérola Rafaella Cedano Godoy,Denis Oliveira Lima,Valéria B de Souza,André A Schenka,Fernanda V Mariano,Felipe Fernandes Jacintho,Idam Hermawan,Hiroaki Shimokawa,Masato Tsutsui,Silvana Chiavegatto,Edson Antunes,Gilberto De Nucci

Scientific reports 15:30559 PubMed40835626

2025

Endothelial function can be modulated by acute hyperglycemia.

Applications

Unspecified application

Species

Unspecified reactive species

Hassanain Qambari,Paula K Yu,Chandrakumar Balaratnasingam,Jayden Dickson,Dao-Yi Yu

Journal of tissue engineering 16:20417314251360755 PubMed40792201

2025

Transplantation of engineered vascularized lymphatic tissue using LEC and in vivo AV loop model to enhance lymphangiogenesis and restore lymphatic drainage in a lymphadenectomy rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Gina A Mackert,Hui-Yi Hsiao,Yung-Chun Chang,Robin T Wu,Rushil R Dang,Richard Tee,Jung-Ju Huang

Nature 643:755-767 PubMed40468071

2025

Increased CSF drainage by non-invasive manipulation of cervical lymphatics.

Applications

Unspecified application

Species

Unspecified reactive species

Hokyung Jin,Jin-Hui Yoon,Seon Pyo Hong,Yu Seok Hwang,Myung Jin Yang,Jieun Choi,Hae Jin Kang,Seung Eun Baek,Cheolhwa Jin,Junho Jung,Hae Jin Kim,Jincheol Seo,Jinyoung Won,Kyung Seob Lim,Chang-Yeop Jeon,Youngjeon Lee,Michael J Davis,Hyung-Soon Park,Donald M McDonald,Gou Young Koh

European journal of pain (London, England) 29:e70047 PubMed40411263

2025

Astrocytic Inducible Nitric Oxide Synthase Upregulation Contributes to Chronic Below-Level Neuropathic Pain Following Spinal Cord Injury in Male Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Youngkyung Kim,Hyunggoo Kang,Young Wook Yoon

Drug design, development and therapy 19:2609-2628 PubMed40196753

2025

Mechanism of the Traditional Chinese Medicine Simiao Biejia Decoction Improves the Diabetes Mellitus-Induced Erectile Dysfunction in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Liu,Dalin Sun,Dong Xing,Yiqi Rui,Yihan Jin,Peng Wang,Bin Cai,Chuyu Li,Chao Gao,Yugui Cui,Baofang Jin

Journal of cellular and molecular medicine 29:e70440 PubMed40045157

2025

Laminarin Alleviates Acute Lung Injury Induced by LPS Through Inhibition of M1 Macrophage Polarisation.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Zeng,Jieyu Zhang,Rongrong Song,Xinhuai Dong,Zibo Wei,Xiaoyan Li,Xiaokang Zeng,Jie Yao

Pathophysiology : the official journal of the International Society for Pathophysiology 32: PubMed39982361

2025

Evolocumab Reduces Oxidative Stress and Lipid Peroxidation in Obese Zucker Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Cebova,Radoslava Bulkova,Olga Pechanova

Medicine 103:e37130 PubMed38306519

2024

Endothelial marker profiles in cerebral radiation-induced vasculopathy: A comparative immunohistochemical analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Mohsen Mosleh,Moon-Jun Sohn,Han Seong Kim

Nature communications 15:735 PubMed38272886

2024

A drug-free cardiovascular stent functionalized with tailored collagen supports in-situ healing of vascular tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Haoshuang Wu,Li Yang,Rifang Luo,Li Li,Tiantian Zheng,Kaiyang Huang,Yumei Qin,Xia Yang,Xingdong Zhang,Yunbing Wang
View all publications

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