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Tags & Cell Markers Cell Type Markers Epi / Endo-thelial

Anti-Endothelium抗体[PAL-E] (ab8086)

  • Datasheet
Reviews (2)Q&A (9)References (11)

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Immunohistochemistry (Frozen sections) - Anti-Endothelium antibody [PAL-E] (ab8086)

    Key features and details

    • Mouse monoclonal [PAL-E] to Endothelium
    • Suitable for: IHC-Fr
    • Reacts with: Rabbit, Goat, Dog, Human, Pig
    • Isotype: IgG2a

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    概述

    • 产品名称

      Anti-Endothelium抗体[PAL-E]
      参阅全部 Endothelium 一抗
    • 描述

      小鼠单克隆抗体[PAL-E] to Endothelium
    • 宿主

      Mouse
    • 特异性

      Reactivity with: Lymphatic endothelium :not detectable. Sinus histiocytes :not detectable. Megakaryocytes :not detectable.
    • 经测试应用

      适用于: IHC-Frmore details
      不适用于: WB
    • 种属反应性

      与反应: Rabbit, Goat, Dog, Human, Pig
      不与反应: Mouse, Rat, Chicken
    • 免疫原

      Tissue, cells or virus corresponding to Human Endothelium.

    • 阳性对照

      • IHC-Fr: human spleen tissue
    • 常规说明


      The antibody only stains human and various animal (goat, rabbit, pig, dog) blood vessels with the exception of arteries.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • 存储溶液

      pH: 7.3
      Preservative: 0.07% Sodium azide
    • Concentration information loading...
    • 纯度

      Tissue culture supernatant
    • Primary antibody说明

      The antibody only stains human and various animal (goat, rabbit, pig, dog) blood vessels with the exception of arteries.
    • 克隆

      单克隆
    • 克隆编号

      PAL-E
    • 同种型

      IgG2a
    • 研究领域

      • Tags & Cell Markers
      • Cell Type Markers
      • Epi / Endo-thelial

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab8086于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-Fr (1)
    1/100.

    Fix with acetone.

    说明
    IHC-Fr
    1/100.

    Fix with acetone.

    应用说明
    Is unsuitable for WB.

    靶标

    • 相关性

      The endothelium is located at the interface between the blood and the vessel wall. The cells are in close contact and form a slick layer that prevents blood cell interaction with the vessel wall as blood moves through the vessel lumen. The endothelium consists of simple squamous epithelium that lines the lumen of all blood vessels. It plays a critical role in the mechanics of blood flow, the regulation of coagulation, leukocyte adhesion, and vascular smooth muscle cell growth, and also serves as a barrier to the transvascular diffusion of liquids and solutes. For years the endothelium was thought of as an inert single layer of cells that passively allow the passage of water and other small molecules across the vessel wall. However, this dynamic tissue performs many other active functions, such as the secretion and modification of vasoactive substances and the contraction and relaxation of vascular smooth muscle.

    图片

    • Immunohistochemistry (Frozen sections) - Anti-Endothelium antibody [PAL-E] (ab8086)
      Immunohistochemistry (Frozen sections) - Anti-Endothelium antibody [PAL-E] (ab8086)
      Immunohistochemistry (Frozen sections) analysis of frozen human sleep tissue section labeling Endothelium with ab8086. The frozen section is fixated using acetone.
       
      The magnification is 100 times.

       

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (11)

    发表研究结果有使用 ab8086?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab8086 被引用在 11 文献中.

    • Elkadri A  et al. Mutations in Plasmalemma Vesicle Associated Protein Result in Sieving Protein-Losing Enteropathy Characterized by Hypoproteinemia, Hypoalbuminemia, and Hypertriglyceridemia. Cell Mol Gastroenterol Hepatol 1:381-394.e7 (2015). PubMed: 26207260
    • Keuschnigg J  et al. Plasticity of blood- and lymphatic endothelial cells and marker identification. PLoS One 8:e74293 (2013). PubMed: 24058540
    • Park SS  et al. Long-term effects of intravitreal injection of GMP-grade bone-marrow-derived CD34+ cells in NOD-SCID mice with acute ischemia-reperfusion injury. Invest Ophthalmol Vis Sci 53:986-94 (2012). PubMed: 22247454
    • Moriyama T  et al. The significance of caveolae in the glomeruli in glomerular disease. J Clin Pathol : (2011). PubMed: 21450752
    • Moussai D  et al. The human cutaneous squamous cell carcinoma microenvironment is characterized by increased lymphatic density and enhanced expression of macrophage-derived VEGF-C. J Invest Dermatol 131:229-36 (2011). PubMed: 20827282
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 11 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Endothelium antibody [PAL-E]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (endothelial (HUVECs))
    Specification
    endothelial (HUVECs)
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton-x100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. S Della Bella

    Verified customer

    提交于 Nov 26 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Endothelium antibody [PAL-E]

    Excellent
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Sheep Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Acetone
    Permeabilization
    Yes - triton-x
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 15°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 24 2012

    Question

    Dear xxxx,
    Thank you for your very kind offer allowing me to test this antibody and to receive a free primary antibody if I submit an abreview. I would very much like to take you up on this offer, and if you send me a discount code, I will order the antibody, and submit an abreview within 4 months
    Thanks very much

    Read More

    Abcam community

    Verified customer

    Asked on Aug 01 2012

    Answer

    I am very pleased to hear you would like to take part in the testing discount scheme. I have generated the discount code as follows:
    Discount code: xxxxxxxxx Expiration date: 2nd December 2012
    Please quote this code in the "Additional comments" section when submitting your Abreview for ab8086 with your ovine samples in IHC of paraffin embedded sections. Once the Abreview has been submitted the code will become activated and you can quote the code when placing the order for your free product. This code expires on the 2nd of December 2012.
    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.
    Until then, I wish you all the best with your research.

    Read More

    Abcam Scientific Support

    回复于 Aug 02 2012

    Question

    Dear Abcam,
    I would like to use the following anitbody for IHC in PFA-fixed ovine brain tissue. I note from your datasheet that this antibody works in goat, so this suggests it might work in sheep too. But it seems that it might not work in fixed tissue - the data sheet only states frozen. I was wondering if you have any other information on the application of this antibody in fixed tissue? Would you be prepared to sell me a small volume to test this?
    Anti-Endothelium antibody [PAL-E] (ab8086)
    Thanks for your help

    Read More

    Abcam community

    Verified customer

    Asked on Aug 01 2012

    Answer

    Thank you for contacting us and your interest in our products.
    As you have seen from the datasheet of the anti-Endothelium antibody [PAL-E] (ab8086), the antibody has not as yet been used in detecting the ovine protein or IHC of paraffin embedded sections as you are hoping to do. This does not mean that it would not work, simply that we have not had any reports supporting it yet.
    Unfortunately we are not able to provide sample sizes of any of our products. This is due to the logistics that would be involved in this, with such a large catalogue this simply isn't feasible. However, we do offer a testing discount scheme. This offer means that if you test the antibody with ovine paraffin embedded tissue and share the results with us through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price off any product in our catalogue). More information on this offer can be found here:
    www.abcam.com/collaborationdiscount
    The only constraint to this offer is that the antibody to be tested must be purchased, tested, the Abreview submitted and the free primary antibody claimed within a 4 month period.
    If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab8086.
    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    回复于 Aug 01 2012

    Question

    Dear Anja,
    It's ok for our customer to test ab8086 in ICC/IF,
    Please find here below our customer's details:



    Please send as soos as possible the discount code so the customer will place the order
    Kind regards

    Read More

    Abcam community

    Verified customer

    Asked on Jul 06 2012

    Answer

    DISCOUNT CODE: 0000000

    Expiration date: 03. November 2012
    I am very pleased to hear your customer would like to accept our offer and test ab8086 in ICC/IF.
    This code will give your customer 1 free primary antibody before the expiration date.
    To redeem this offer, please submit an Abreview for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests.
    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
    1) Call to place your order and mention the code to our customer service department;
    2) Include the code in your fax order;
    3) Place your order on the web and enter the promotional code.
    Any feedback that your customer can provide will be greatly appreciated, whether positive or negative. If you or your customer has any further questions, please do not hesitate to contact us. We look forward to receiving your customer's Abreview and wish your customer luck with their research.
    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    回复于 Jul 06 2012

    Question

    Thanks for your reply. The customer would be interested in using ab8086 but it is not tested for IF.
    The customer has to use this Ab for IF (confocal microscopy) in human samples, so she would like to know if you can send me a discount code for parteciapating to your Abreview program.
    Please let me know, it is very urgent
    Kind regards

    Read More

    Abcam community

    Verified customer

    Asked on Jul 05 2012

    Answer

    Thank you and your customer very much for your interest in ab8086.
    To our knowledge, ab8086 has not been tested in ICC/IF. Therefore, I can offer a discount off a future purchase if your customer buys ab8086 now, tests it in ICC/IF and submits feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your customer's feedback. The discount would be to the value of ab8086 and can be used for the next order of any product.
    If your customer is interested in this offer, please follow these steps:
    1. Reply to this e-mail to let me know that your customer ( please include their e-mail address) would like to proceed and test ab8086 in ICC/IF. I will then send a discount code. This code must be issued before purchasing ab8086 so please wait for my reply before ordering.
    2. Purchase ab8086 either by phone, fax, or online (www.abcam.com).
    3. Test it in ICC/IF.
    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
    5. After the review is submitted to us, the discount code becomes active. Simply place your customer's new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue.
    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab8086 turns out to be unsuitable for ICC/IF, your customer will still receive the discount on their next purchase after your Abreview has been submitted.
    Please let me know if you or your customer have any questions about this offer and I would be happy to help you further.
    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    回复于 Jul 05 2012

    Question

    Dear All,

    Code ab128058 is not available in stock and I found this code ab8086 as possible alternative (same clone).
    Can you confirm this and give any other possible alternatives? Moreover can you confirm also availability for this altetrnative? It is very urgent
    Thanks in advance and kind regards
    ____________________________________________

    Read More

    Abcam community

    Verified customer

    Asked on Jul 04 2012

    Answer

    Thank you for your email.
    I can confirm that you are correct and ab8086 is the same clone as ab128058 and therefore the best alternative for your customer.
    Please let me know what application and species your customer is using if you have any more questions in this matter.

    Read More

    Abcam Scientific Support

    回复于 Jul 04 2012

    Question

    I am interested to tag indocyanine green (ICG) with PAL-E to study the photochemical effects in vascular systems. I would like to know the procedures to tag ICG with PAL-E.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 27 2003

    Answer

    Thank you for your enquiry. All the information we having regarding this antibody is located on the online datasheet. I am sorry but we do not have a protocol for tagging ICG with PAL-E. If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Oct 27 2003

    Question

    I would like to use this antibody of formalin fixed paraffin embedded sections. We have already purchased lots of antibodies that are not satisfactory. Is there any way that you could provide us with a small amount of antibody to test? Obviously if we are happy with it and it works in our system will will be buying full vials of it in the future. Many thanks

    Read More

    Abcam community

    Verified customer

    Asked on Oct 13 2003

    Answer

    Dear Kavita datasheets. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

    Read More

    Asdf Edo

    Abcam Scientific Support

    回复于 Oct 13 2003

    Question

    Regarding ab8086, do you know whether it produces reasonable results in formalin-fixed paraffin embedded tissue and whether/what type of antigen retrieval methods are best? Many thanks

    Read More

    Abcam community

    Verified customer

    Asked on Jun 18 2002

    Answer

    We do not have any data on the antibodies use in formalin-fixed paraffin embedded tissue and believe that it has not yet been tested in this application. If we can find further information we will contact you with this.

    Read More

    Abcam Scientific Support

    回复于 Jun 19 2002

    1-10 of 11 Abreviews or Q&A

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