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AB8086

Anti-Endothelium抗体[PAL-E]

Anti-Endothelium antibody [PAL-E]

3

(2 Reviews)

|

(12 Publications)

Mouse Monoclonal Endothelium antibody. Suitable for IHC-Fr and reacts with Human, Pig, Dog, Goat, Rabbit samples. Cited in 12 publications.
1 Images
Immunohistochemistry (Frozen sections) - Anti-Endothelium antibody [PAL-E] (AB8086)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Endothelium antibody [PAL-E] (AB8086)

Immunohistochemistry (Frozen sections) analysis of frozen human sleep tissue section labeling Endothelium with ab8086. The frozen section is fixated using acetone.

The magnification is 100 times.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

PAL-E

亚型

IgG2a

不含载体蛋白

No

反应种属

Rabbit, Goat, Dog, Human, Pig

应用

IHC-Fr

applications

特异性

Reactivity with: Lymphatic endothelium :not detectable. Sinus histiocytes :not detectable. Megakaryocytes :not detectable.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>Fix with acetone.</p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>Fix with acetone.</p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>Fix with acetone.</p>" }, "Chicken": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>Fix with acetone.</p>" }, "Dog": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>Fix with acetone.</p>" }, "Goat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>Fix with acetone.</p>" }, "Pig": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>Fix with acetone.</p>" }, "Rabbit": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>Fix with acetone.</p>" } } }
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产品详情

The antibody only stains human and various animal (goat, rabbit, pig, dog) blood vessels with the exception of arteries.
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性能和储存信息

形式
Liquid
纯度
Tissue culture supernatant
存储溶液
pH: 7.3 Preservative: 0.07% Sodium azide
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The endothelium a monolayer of cells lining blood vessels plays an important role in vascular function and maintenance. Endothelial cells have a mass of approximately 35 kDa and are found in arteries veins and capillaries including the heart and lymphatic vessels. These cells form a selective barrier between the bloodstream and surrounding tissues regulating the exchange of fluids solutes and macromolecules. The endothelium also modulates blood flow and pressure through interaction with vascular smooth muscle cells.
Biological function summary

The endothelium contributes to several critical processes including vascular homeostasis coagulation and inflammation regulation. It operates as part of the vascular system interacting with components like platelets and leukocytes to mediate immune responses. The endothelium produces signaling molecules such as nitric oxide (NO) which is essential for vasodilation. This layer also responds to mechanical stimuli from blood flow adapting its structure and function accordingly.

Pathways

The endothelium engages in the nitric oxide and endothelin pathways which balance vasodilation and vasoconstriction. In the nitric oxide pathway the enzyme endothelial nitric oxide synthase (eNOS) becomes activated producing NO that signals smooth muscle relaxation. Within the endothelin pathway endothelin-1 interacts with vascular smooth muscle cells causing vasoconstriction. Both pathways are integral to maintaining vascular tone and regulating blood pressure.

The endothelium's dysfunction is associated with conditions such as atherosclerosis and hypertension. In atherosclerosis the impaired endothelial function leads to plaque formation and arterial stiffness. Hypertension involves chronic constriction and reduced NO bioavailability affecting endothelium-mediated vasodilation. Related proteins include low-density lipoprotein (LDL) for atherosclerosis and angiotensin-converting enzyme (ACE) in hypertension both impacting endothelial health and function.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information Endothelium
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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107966 PubMed37810232

2023

The senescent secretome drives PLVAP expression in cultured human hepatic endothelial cells to promote monocyte transmigration.

Applications

Unspecified application

Species

Unspecified reactive species

Alex L Wilkinson,Samuel Hulme,James I Kennedy,Emily R Mann,Paul Horn,Emma L Shepherd,Kelvin Yin,Marco Y W Zaki,Gareth Hardisty,Wei-Yu Lu,Pia Rantakari,David H Adams,Marko Salmi,Matthew Hoare,Daniel A Patten,Shishir Shetty

Cellular and molecular gastroenterology and hepatology 1:381-394.e7 PubMed26207260

2015

Mutations in Plasmalemma Vesicle Associated Protein Result in Sieving Protein-Losing Enteropathy Characterized by Hypoproteinemia, Hypoalbuminemia, and Hypertriglyceridemia.

Applications

Unspecified application

Species

Unspecified reactive species

Abdul Elkadri,Cornelia Thoeni,Sophie J Deharvengt,Ryan Murchie,Conghui Guo,James D Stavropoulos,Christian R Marshall,Paul Wales,Robert Bandsma,Ernest Cutz,Chaim M Roifman,David Chitayat,Yaron Avitzur,Radu V Stan,Aleixo M Muise

PloS one 8:e74293 PubMed24058540

2013

Plasticity of blood- and lymphatic endothelial cells and marker identification.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Keuschnigg,Sirkku Karinen,Kaisa Auvinen,Heikki Irjala,John-Patrick Mpindi,Olli Kallioniemi,Sampsa Hautaniemi,Sirpa Jalkanen,Marko Salmi

Investigative ophthalmology & visual science 53:986-94 PubMed22247454

2012

Long-term effects of intravitreal injection of GMP-grade bone-marrow-derived CD34+ cells in NOD-SCID mice with acute ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Susanna S Park,Sergio Caballero,Gerhard Bauer,Bradley Shibata,Alan Roth,Paul G Fitzgerald,Krisztina I Forward,Ping Zhou,Jeannine McGee,David G Telander,Maria B Grant,Jan A Nolta

Journal of clinical pathology 64:504-9 PubMed21450752

2011

The significance of caveolae in the glomeruli in glomerular disease.

Applications

Unspecified application

Species

Unspecified reactive species

Takahito Moriyama,Yuki Tsuruta,Ari Shimizu,Mitsuyo Itabashi,Takashi Takei,Shigeru Horita,Keiko Uchida,Kosaku Nitta

The Journal of investigative dermatology 131:229-36 PubMed20827282

2010

The human cutaneous squamous cell carcinoma microenvironment is characterized by increased lymphatic density and enhanced expression of macrophage-derived VEGF-C.

Applications

Unspecified application

Species

Unspecified reactive species

Dariush Moussai,Hiroshi Mitsui,Julia S Pettersen,Katherine C Pierson,Kejal R Shah,Mayte Suárez-Fariñas,Irma R Cardinale,Mark J Bluth,James G Krueger,John A Carucci

Diabetes 56:960-7 PubMed17395742

2007

Ischemic vascular damage can be repaired by healthy, but not diabetic, endothelial progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sergio Caballero,Nilanjana Sengupta,Aqeela Afzal,Kyung-Hee Chang,Sergio Li Calzi,Dennis L Guberski,Timothy S Kern,Maria B Grant

Blood 106:3405-9 PubMed16099878

2005

Molecular identification of PAL-E, a widely used endothelial-cell marker.

Applications

Unspecified application

Species

Unspecified reactive species

Harri Niemelä,Kati Elima,Tiina Henttinen,Heikki Irjala,Marko Salmi,Sirpa Jalkanen

Blood 104:3849-57 PubMed15297319

2004

CLEVER-1 mediates lymphocyte transmigration through vascular and lymphatic endothelium.

Applications

Unspecified application

Species

Unspecified reactive species

Marko Salmi,Kaisa Koskinen,Tiina Henttinen,Kati Elima,Sirpa Jalkanen

Journal of immunological methods 91:45-52 PubMed3014005

1986

The detection of endothelial cell antigens in cutaneous tissue using methacarn and periodate lysine paraformaldehyde fixation.

Applications

Unspecified application

Species

Unspecified reactive species

C A Holden,J Spaull,R Williams,C J Spry,R R Jones,E W Jones
View all publications
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关联产品

Select an associated product type
Alternative Product
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