Anti-Endothelium抗体[EN4] (ab8087)
Key features and details
- Mouse monoclonal [EN4] to Endothelium
- Suitable for: IHC-Fr, IHC-P, WB
- Reacts with: Guinea pig, Cat, Human
- Isotype: IgG1
概述
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产品名称
Anti-Endothelium抗体[EN4]
参阅全部 Endothelium 一抗 -
描述
小鼠单克隆抗体[EN4] to Endothelium -
宿主
Mouse -
特异性
This antibody stains all human blood vessels including brain microvessels. Both large and small vessels are equally reactive. The EN4 antigen is preserved in endothelial cells in culture unlike the PAL-E antigen (ab8086) which is lost. It stains strongly murine fibroblasts transfected with the human CD31 gene. On surface-iodinated Jurkat T cells it recognizes the 130 kD CD31 antigen. EN4 also binds to guinea-pig and cat endothelium, but not to rabbit, bovine, sheep, dog, rat or mice endothelial cells. No other structures in the skin, heart, kidney, tonsils or spleen are stained with this antibody. -
经测试应用
适用于: IHC-Fr, IHC-P, WBmore details -
种属反应性
与反应: Guinea pig, Cat, Human
不与反应: Mouse, Sheep, Rabbit, Cow, Dog -
常规说明
The antibody is useful for immunohistological staining of endothelia. Lymphatic endothelium is stained (unlike the PAL-E antibody). Kaposi's sarcoma and other vascular tumors of endothelial cell origin are also stained.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.3
Preservative: 0.02% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
纯度
Tissue culture supernatant -
Primary antibody说明
The antibody is useful for immunohistological staining of endothelia. Lymphatic endothelium is stained (unlike the PAL-E antibody). Kaposi's sarcoma and other vascular tumors of endothelial cell origin are also stained. -
克隆
单克隆 -
克隆编号
EN4 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. PubMed: 21645391
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| 说明 |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. PubMed: 21645391 |
靶标
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相关性
The endothelium is located at the interface between the blood and the vessel wall. The cells are in close contact and form a slick layer that prevents blood cell interaction with the vessel wall as blood moves through the vessel lumen. The endothelium consists of simple squamous epithelium that lines the lumen of all blood vessels. It plays a critical role in the mechanics of blood flow, the regulation of coagulation, leukocyte adhesion, and vascular smooth muscle cell growth, and also serves as a barrier to the transvascular diffusion of liquids and solutes. For years the endothelium was thought of as an inert single layer of cells that passively allow the passage of water and other small molecules across the vessel wall. However, this dynamic tissue performs many other active functions, such as the secretion and modification of vasoactive substances and the contraction and relaxation of vascular smooth muscle.
数据表及文件
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Datasheet download
文献 (6)
ab8087 被引用在 6 文献中.
- Siemerink MJ et al. CD34 marks angiogenic tip cells in human vascular endothelial cell cultures. Angiogenesis 15:151-63 (2012). PubMed: 22249946
- Lasagna-Reeves CA et al. Tau oligomers impair memory and induce synaptic and mitochondrial dysfunction in wild-type mice. Mol Neurodegener 6:39 (2011). WB ; Mouse . PubMed: 21645391
- Péqueux C et al. Oxytocin receptor pattern of expression in primary lung cancer and in normal human lung. Lung Cancer 50:177-88 (2005). IHC-Fr ; Human . PubMed: 16043261
- Burgio VL et al. Characterization of EN4 monoclonal antibody: a reagent with CD31 specificity. Clin Exp Immunol 96:170-6 (1994). PubMed: 7512006
- Holden CA et al. The detection of endothelial cell antigens in cutaneous tissue using methacarn and periodate lysine paraformaldehyde fixation. J Immunol Methods 91:45-52 (1986). PubMed: 3014005
- Jones RR et al. Histogenesis of Kaposi's sarcoma in patients with and without acquired immune deficiency syndrome (AIDS). J Clin Pathol 39:742-9 (1986). PubMed: 3090109
