Anti-Endomucin抗体[V.7C7.1] (ab106100)
![Immunocytochemistry/ Immunofluorescence - Anti-Endomucin antibody [V.7C7.1] (ab106100)](https://www.abcam.cn/ps/products/106/ab106100/Images/ab106100-314095-anti-endomucin-antibody-v7c71-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Endomucin antibody [V.7C7.1] (ab106100)")
Key features and details
- Rat monoclonal [V.7C7.1] to Endomucin
- Suitable for: IHC-Fr, IHC-P, IP, Flow Cyt, WB, IHC - Wholemount, ICC/IF
- Reacts with: Mouse
- Isotype: IgG2a
概述
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产品名称
Anti-Endomucin抗体[V.7C7.1]
参阅全部 Endomucin 一抗 -
描述
大鼠单克隆抗体[V.7C7.1] to Endomucin -
宿主
Rat -
经测试应用
适用于: IHC-Fr, IHC-P, IP, Flow Cyt, WB, IHC - Wholemount, ICC/IFmore details
不适用于: FuncS -
种属反应性
与反应: Mouse
不与反应: Human -
免疫原
Other Immunogen Type corresponding to Mouse Endomucin. Mouse endomucin-IgG fusion protein
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
0.2 µm filtered solution -
克隆
单克隆 -
克隆编号
V.7C7.1 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106100于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| IHC-P | (4) |
Use at an assay dependent concentration.
|
| IP |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
| WB |
Use at an assay dependent concentration.
|
|
| IHC - Wholemount | (1) |
Use at an assay dependent concentration.
|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
| 说明 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
WB
Use at an assay dependent concentration. |
|
IHC - Wholemount
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix. -
组织特异性
Expressed in heart, kidney and lung. -
翻译后修饰
Highly O-glycosylated. Sialic acid-rich glycoprotein. -
细胞定位
Secreted and Cell membrane. Membrane. Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks. - Information by UniProt
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数据库链接
- Entrez Gene: 59308 Mouse
- SwissProt: Q9R0H2 Mouse
- Unigene: 27343 Mouse
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别名
- EMCN antibody
- EMCN2 antibody
- Endomucin 2 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Endomucin antibody [V.7C7.1] (ab106100)
Immuno fluorescence staining of bEND3 cells with ab106100 at a concentration of 1 μg/ml.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (57)
ab106100 被引用在 57 文献中.
- Himmels P et al. T cell-dependent bispecific antibodies alter organ-specific endothelial cell-T cell interaction. EMBO Rep 24:e55532 (2023). PubMed: 36621885
- Tang AT et al. Cell-autonomous requirement for ACE2 across organs in lethal mouse SARS-CoV-2 infection. PLoS Biol 21:e3001989 (2023). PubMed: 36745682
- Wu X et al. Generation of Conditional Knockout Alleles for PRUNE-1. Cells 12:N/A (2023). PubMed: 36831191
- Zhou T et al. Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single-Cell Resolution. Adv Sci (Weinh) 10:e2206296 (2023). PubMed: 36965031
- Panebianco C et al. Counteracting gemcitabine+nab-paclitaxel induced dysbiosis in KRAS wild type and KRASG12D mutated pancreatic cancer in vivo model. Cell Death Discov 9:116 (2023). PubMed: 37019893