AB76122

重组Anti-Endo G抗体[EP1665Y]

Anti-Endo G antibody [EP1665Y]

RabMAb
Recombinant
KO Validated
了解详情

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(10 Publications)

Rabbit Recombinant Monoclonal Endo G antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 10 publications.

查看别名

Endo G, ENDOG

2 Images

Unknown

Western blot - Anti-Endo G antibody [EP1665Y] (AB76122)

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Western blot - Anti-Endo G antibody [EP1665Y] (ab76122) at 1/2000 dilution

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HepG2 cell lysate at 10 µg

Secondary

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HRP labelled goat anti-rabbit at 1/1000 dilution

Predicted band size: 33 kDa

Observed band size: 35 kDa

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Lab

Western blot - Anti-Endo G antibody [EP1665Y] (AB76122)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Endo G HAP1 cell lysate (20 μg)
Lane 3 : Mouse liver tissue lysate (20 μg)
Lane 4 : HepG2 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab76122 observed at 29 kDa. Red - loading control, ab7291, observed at 52 kDa.

ab76122 was shown to specifically react with Endo G when Endo G knockout samples were used. Wild-type and Endo G knockout samples were subjected to SDS-PAGE. ab76122 and ab7291 (loading control to alpha tubulin) were diluted 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)

Predicted band size: 33 kDa

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不同偶联物与剂型 (1)

Carrier free

Anti-Endo G antibody [EP1665Y] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP1665Y

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Negative results for HepG2/293T (2% PFA 20min fixation)", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "HepG2 cells, no signal detected", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Negative results for HepG2/mouse liver/rat liver/NIH3T3 (2% PFA 20min fixation)", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Negative results for HepG2/293T (2% PFA 20min fixation)", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "HepG2 cells, no signal detected", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Negative results for HepG2/mouse liver/rat liver/NIH3T3 (2% PFA 20min fixation)", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Endonuclease G (Endo G) is an important mitochondrial nuclease known for its role in DNA and RNA metabolism. Endo G is also referred to as endoglycosidase S and has a molecular mass of approximately 28 kDa. This protein is expressed mainly in the mitochondria but can translocate to the nucleus under certain conditions. It functions by cleaving DNA and RNA which facilitates the processing of nucleic acids in the cell. Endo G exhibits endonuclease activity making it a significant factor in nucleic acid regulation.

Biological function summary

This target plays an essential role in mitochondrial DNA fragmentation during apoptosis. Endo G operates independently of caspases highlighting its role as a non-canonical mediator of apoptosis. It becomes active upon cellular stress usually through the release from the mitochondria where it functions as part of a larger apoptotic complex. This characteristic grants it an important function in programmed cell death mechanisms.

Pathways

Endo G integrates into apoptosis and mitochondrial biogenesis. This protein participates in the intrinsic pathway of apoptosis collaborating with other proteins like apoptosis-inducing factor (AIF) to execute cell death. In the context of mitochondrial biogenesis Endo G involvement suggests potential interaction with proteins that oversee DNA replication and transcription influencing both apoptosis and cellular energy pathways.

Defects or dysregulation in Endo G activity associate with various cancer types and neurodegenerative conditions. In cancer increased Endo G levels correlate with heightened apoptosis emphasizing its potential role in cancer therapy and as a prognostic marker. In neurodegenerative diseases like Parkinson's Endo G may contribute to neuronal cell death interacting with proteins involved in mitochondrial homeostasis. These interactions present insights into therapeutic targets for related conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA (PubMed : 25355512). The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs (By similarity). Shows significantly higher affinity for four-stranded Holliday junction over duplex and single-stranded DNAs (By similarity). Promotes conservative recombination when the DNA is 5hmC-modified (PubMed : 25355512). Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response (PubMed : 33473107). GSK3-beta mediated phosphorylation of ENDOG enhances its interaction with YWHAG, leading to the release of TSC2 and PIK3C3 from YWHAG resulting in mTOR pathway suppression and autophagy initiation (PubMed : 33473107). Promotes cleavage of mtDNA in response to oxidative and nitrosative stress, in turn inducing compensatory mtDNA replication (PubMed : 29719607).

See full target information Endonuclease G, mitochondrial

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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 122:e2411298122 PubMed39752519

2025

Endonuclease G promotes hepatic mitochondrial respiration by selectively increasing mitochondrial tRNA production.

Applications

Unspecified application

Species

Unspecified reactive species

Xihui Xu,Rozhin Penjweini,Lóránt Székvölgyi,Zsolt Karányi,Anne-Marie Heckel,Devikala Gurusamy,Dóra Varga,Shutong Yang,Alexandra L Brown,Wenqi Cui,Jinsung Park,Dénes Nagy,Maren C Podszun,Sarah Yang,Komudi Singh,Stephen P Ashcroft,Jeonghan Kim,Myung K Kim,Ivan Tarassov,Jun Zhu,Andrew Philp,Yaron Rotman,Jay R Knutson,Nina Entelis,Jay H Chung

International journal of molecular sciences 25: PubMed39519238

2024

Melittin Inhibits Colorectal Cancer Growth and Metastasis by Ac-Tivating the Mitochondrial Apoptotic Pathway and Suppressing Epithelial-Mesenchymal Transition and Angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Kangli Wang,Lingchen Tao,Meifei Zhu,Xinyu Yu,Yuanyuan Lu,Bin Yuan,Fuliang Hu

PloS one 19:e0294187 PubMed38227585

2024

TET3 is a positive regulator of mitochondrial respiration in Neuro2A cells.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Leon Kropf,Caraugh J Albany,Anna Zoccarato,Hannah L H Green,Youwen Yang,Alison C Brewer

Cancers 13: PubMed34359707

2021

ENDOG Impacts on Tumor Cell Proliferation and Tumor Prognosis in the Context of PI3K/PTEN Pathway Status.

Applications

Unspecified application

Species

Unspecified reactive species

Gisel Barés,Aida Beà,Luís Hernández,Raul Navaridas,Isidre Felip,Cristina Megino,Natividad Blasco,Ferran Nadeu,Elías Campo,Marta Llovera,Xavier Dolcet,Daniel Sanchis

Redox biology 37:101736 PubMed33032073

2020

Involvement of the mitochondrial nuclease EndoG in the regulation of cell proliferation through the control of reactive oxygen species.

Applications

Unspecified application

Species

Unspecified reactive species

Natividad Blasco,Aida Beà,Gisel Barés,Cristina Girón,Raúl Navaridas,Andrea Irazoki,Guillermo López-Lluch,Antonio Zorzano,Xavier Dolcet,Marta Llovera,Daniel Sanchis

Oxidative medicine and cellular longevity 2020:1359164 PubMed32411316

2020

Copper Induces Oxidative Stress and Apoptosis in the Mouse Liver.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Liu,Hongrui Guo,Zhijie Jian,Hengmin Cui,Jing Fang,Zhicai Zuo,Junliang Deng,Yinglun Li,Xun Wang,Ling Zhao

International immunopharmacology 75:105836 PubMed31450153

2019

TRPM2 channel regulates cytokines production in astrocytes and aggravates brain disorder during lipopolysaccharide-induced endotoxin sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhu,Yisha Zhao,Hui Hu,Qianqian Zheng,Xiaoying Luo,Yinjie Ling,Yingchao Ying,Zheng Shen,Peifang Jiang,Qiang Shu

Toxicology research 7:792-808 PubMed30310657

2018

The mitochondrial pathway is involved in sodium fluoride (NaF)-induced renal apoptosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Wei,Qin Luo,Huan Liu,Linlin Chen,Hengmin Cui,Jing Fang,Zhicai Zuo,Junliang Deng,Yinglun Li,Xun Wang,Ling Zhao

International journal of molecular sciences 18: PubMed28272306

2017

AKT2 Blocks Nucleus Translocation of Apoptosis-Inducing Factor (AIF) and Endonuclease G (EndoG) While Promoting Caspase Activation during Cardiac Ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Yang,Xinmei Zhao,Hui Xu,Fan Chen,Yitao Xu,Zhe Li,Daniel Sanchis,Liang Jin,Yubin Zhang,Junmei Ye

Endocrinology 157:3873-3887 PubMed27547848

2016

EndoG Knockout Mice Show Increased Brown Adipocyte Recruitment in White Adipose Tissue and Improved Glucose Homeostasis.

Applications

Unspecified application

Species

Mouse

Rosario Pardo,Natividad Blasco,Maria Vilà,Daniel Beiroa,Rubén Nogueiras,Xavier Cañas,Rafael Simó,Daniel Sanchis,Josep A Villena

View all publications

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重组Anti-Endo G抗体[EP1665Y]