重组Anti-ENC1抗体[EPR5406(B)] (ab124902)
![Western blot - Anti-ENC1 antibody [EPR5406(B)] (ab124902)](https://www.abcam.cn/ps/products/124/ab124902/Images/ab124902-138876-anti-enc1-antibody-epr5406b-western-blot.jpg "Western blot - Anti-ENC1 antibody [EPR5406(B)] (ab124902)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5406(B)] to ENC1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ENC1抗体[EPR5406(B)]
参阅全部 ENC1 一抗 -
描述
兔单克隆抗体[EPR5406(B)] to ENC1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human ENC1 aa 550-650. The exact sequence is proprietary.
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阳性对照
- Human fetal brain lysate; Human brain tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.03 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR5406(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124902于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
应用说明
Is unsuitable for IP.
靶标
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功能
Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. May be down-regulated in neuroblastoma tumors. Substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. -
组织特异性
Detected in fetal brain tissue, moderate expression in fetal heart, lung and kidney. Highly expressed in adult brain, particularly high in the hippocampus and amygdala, and spinal chord. Detectable in adult pancreas. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats. -
发展阶段
Dramatically up-regulated upon neuronal differentiation. -
翻译后修饰
Ubiquitinated and probably targeted for proteasome-independent degradation. -
细胞定位
Nucleus matrix. Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 8507 Human
- Entrez Gene: 13803 Mouse
- Entrez Gene: 294674 Rat
- Omim: 605173 Human
- SwissProt: O14682 Human
- SwissProt: O35709 Mouse
- Unigene: 104925 Human
- Unigene: 241073 Mouse
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别名
- CCL 28 antibody
- CCL28 antibody
- Ectoderm neural cortex protein 1 antibody
see all
图片
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Western blot - Anti-ENC1 antibody [EPR5406(B)] (ab124902)Anti-ENC1 antibody [EPR5406(B)] (ab124902) at 1/1000 dilution + Human brain fetal lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENC1 antibody [EPR5406(B)] (ab124902)](https://www.abcam.cn/ps/products/124/ab124902/Images/ab124902-138875-anti-enc1-antibody-epr5406b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENC1 antibody [EPR5406(B)] (ab124902)ab124902, at 1/100 dilution, staining ENC1 in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-ENC1 antibody [EPR5406(B)] (ab124902)](https://www.abcam.cn/ps/products/124/ab124902/Images/ab124902-138874-anti-enc1-antibody-epr5406b.jpg)
OI-RD Scanning - Anti-ENC1 antibody [EPR5406(B)] (ab124902) -
![Anti-ENC1 antibody [EPR5406(B)] (ab124902)](https://www.abcam.cn/ps/products/124/ab124902/Images/ab124902-4-benefits-of-recombinant-antibodies.png)
Anti-ENC1 antibody [EPR5406(B)] (ab124902)
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab124902 被引用在 3 文献中.
- Wu C et al. Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway. Int J Mol Med 47:N/A (2021). PubMed: 33693958
- Gong L et al. The mevalonate coordinates energy input and cell proliferation. Cell Death Dis 10:327 (2019). PubMed: 30975976
- Feng J et al. Identification of a subtype-specific ENC1 gene related to invasiveness in human pituitary null cell adenoma and oncocytomas. J Neurooncol 119:307-15 (2014). PubMed: 24916845