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AB124902

重组Anti-ENC1抗体[EPR5406(B)]

Anti-ENC1 antibody [EPR5406(B)]

3

(2 Reviews)

|

(3 Publications)

Rabbit Recombinant Monoclonal ENC1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.

查看别名

KLHL37, NRPB, PIG10, ENC1, Ectoderm-neural cortex protein 1, ENC-1, Kelch-like protein 37, Nuclear matrix protein NRP/B, p53-induced gene 10 protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENC1 antibody [EPR5406(B)] (AB124902)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENC1 antibody [EPR5406(B)] (AB124902)

ab124902 at 1/100 dilution staining ENC1 in paraffin-embedded Human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-ENC1 antibody [EPR5406(B)] (AB124902)
  • WB

Unknown

Western blot - Anti-ENC1 antibody [EPR5406(B)] (AB124902)

All lanes:

Western blot - Anti-ENC1 antibody [EPR5406(B)] (ab124902) at 1/1000 dilution

All lanes:

Human brain fetal lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 66 kDa

Observed band size: 66 kDa

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OI-RD Scanning - Anti-ENC1 antibody [EPR5406(B)] (AB124902)
  • OI-RD Scanning

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OI-RD Scanning - Anti-ENC1 antibody [EPR5406(B)] (AB124902)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5406(B)

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ENC1 also known as Ectodermal-Neural Cortex 1 weighs approximately 67 kDa. It functions mechanically as part of the ubiquitin-proteasome pathway by acting as a scaffold protein and possessing E3 ligase activity. It facilitates the ubiquitination and proteasomal degradation of target proteins regulating protein turnover. ENC1 expresses widely across different tissue types with significant expression in neuronal tissues which suggests it plays a role in brain function.
Biological function summary

ENC1 influences cellular proliferation differentiation and apoptosis. It is part of the BTB-Kelch protein family and interacts with actin and other cytoskeletal components affecting cell shape and movement. Although not always described as part of a large complex ENC1 interacts with numerous proteins linked to the cytoskeleton and transcription mediating changes that can shift cell behavior in response to developmental signals.

Pathways

ENC1 participates intricately in the Wnt signaling pathway which influences cell fate and patterning during development. ENC1 may interact with proteins like β-catenin linking it to the regulation of gene expression important for developmental processes. It also shows connections to the Notch signaling pathway playing a role in controlling cell differentiation and identity particularly in neural contexts.

ENC1 relates to neurodegenerative diseases and certain cancers. Its expression levels correlate with the progression of some types of cancer where aberrant signaling through ENC1 may disrupt normal cell cycle control. In neurological health its dysfunction associates with conditions such as Alzheimer's disease possibly through altered ubiquitin-proteasome system activity. Proteins like APP (amyloid precursor protein) may help connect ENC1 to neurodegenerative processes due to their common degradation pathways.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. Down-regulates transcription factor NF2L2/NRF2 by decreasing the rate of protein synthesis and not via a ubiquitin-mediated proteasomal degradation mechanism.
See full target information ENC1
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 47: PubMed33693958

2021

Ectodermal‑neural cortex 1 affects the biological function of lung cancer through the MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chengwei Wu,Xianghai Wang,Xingwei Wu,Xingwu Chen

Cell death & disease 10:327 PubMed30975976

2019

The mevalonate coordinates energy input and cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Li Gong,Yi Xiao,Fan Xia,Pei Wu,Tingting Zhao,Shulin Xie,Ran Wang,Qiaocheng Wen,Wensu Zhou,Huilan Xu,Lingyan Zhu,Zeqi Zheng,Tianlun Yang,Zihua Chen,Qiong Duan

Journal of neuro-oncology 119:307-15 PubMed24916845

2014

Identification of a subtype-specific ENC1 gene related to invasiveness in human pituitary null cell adenoma and oncocytomas.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Feng,Lichuan Hong,Yonggang Wu,Chuzhong Li,Hong Wan,Guilin Li,Yilin Sun,Shenyuan Yu,Prashant Chittiboina,Blake Montgomery,Zhengping Zhuang,Yazhuo Zhang
View all publications
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